Good laboratory practice-compliant bio-efficacy testing of repellent products against Aedes aegypti

Rosilawati, Ratmi; Aznieda, A. Ruziyatul; Roziah, A; Hamid, Nurulhusna Ab; Teh, Chien Huey; Siti-Futri, F F; Asuad, M. Khairul; Azahari, A. H.; Fatihah, M. Nurul; Ariffin, M. Muhamad; Cheong, Young-Keun; Nazni, W. A.; Lee, H. L.

doi:10.1007/s00769-021-01455-x

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“…Standardisation of rearing conditions is especially important for strains used to test products such as chlorfenapyr, where metabolic activity is important in activating the insecticide and affected by rearing conditions and conditions during testing [ 25 , 65 ]. Although it may be unrealistic to ask for a single rearing SOP to be used between facilities, most facilities use some form of SOP to achieve standardised rearing and perform quality control (QC) measures, particularly those with GLP accreditation [ 66 , 67 ]. There are guidance documents available [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

Strain Characterisation for Measuring Bioefficacy of ITNs Treated with Two Active Ingredients (Dual-AI ITNs): Developing a Robust Protocol by Building Consensus

Lees

Armistead

Azizi

et al. 2022

Insects

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Durability monitoring of insecticide-treated nets (ITNs) containing a pyrethroid in combination with a second active ingredient (AI) must be adapted so that the insecticidal bioefficacy of each AI can be monitored independently. An effective way to do this is to measure rapid knock down of a pyrethroid-susceptible strain of mosquitoes to assess the bioefficacy of the pyrethroid component and to use a pyrethroid-resistant strain to measure the bioefficacy of the second ingredient. To allow robust comparison of results across tests within and between test facilities, and over time, protocols for bioefficacy testing must include either characterisation of the resistant strain, standardisation of the mosquitoes used for bioassays, or a combination of the two. Through a series of virtual meetings, key stakeholders and practitioners explored different approaches to achieving these goals. Via an iterative process we decided on the preferred approach and produced a protocol consisting of characterising mosquitoes used for bioefficacy testing before and after a round of bioassays, for example at each time point in a durability monitoring study. We present the final protocol and justify our approach to establishing a standard methodology for durability monitoring of ITNs containing pyrethroid and a second AI.

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Section: Resultsmentioning

confidence: 99%