Good vibrations for bad bacteria

McKendry, Rachel A.; Kappeler, Natascha

doi:10.1038/nnano.2013.127

Cited by 5 publications

(8 citation statements)

References 9 publications

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“…Researches by Longo et al [65] indicated that AFM cantilevers can be used to detect low concentrations of bacteria and characterize their metabolism quickly (within minutes) in response to antibiotics, providing a new rapid detection method for drug screening. However, it should be noted that the fluctuations were generated by a population of bacteria, and further research could utilize AFM to track fluctuations of single bacterium [66].…”

Section: Principle and Methods Of Afm Indentation Techniquesmentioning

confidence: 99%

Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy

Liu

et al. 2014

Chin. Sci. Bull.

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The advent of atomic force microscopy (AFM) provides a powerful tool for investigating the behaviors of single living cells under near physiological conditions. Besides acquiring the images of cellular ultra-microstructures with nanometer resolution, the most remarkable advances are achieved on the use of AFM indenting technique to quantify the mechanical properties of single living cells. By indenting single living cells with AFM tip, we can obtain the mechanical properties of cells and monitor their dynamic changes during the biological processes (e.g., after the stimulation of drugs). AFM indentation-based mechanical analysis of single cells provides a novel approach to characterize the behaviors of cells from the perspective of biomechanics, considerably complementing the traditional biological experimental methods. Now, AFM indentation technique has been widely used in the life sciences, yielding a large amount of novel information that is meaningful to our understanding of the underlying mechanisms that govern the cellular biological functions. Here, based on the authors' own researches on AFM measurement of cellular mechanical properties, the principle and method of AFM indentation technique was presented, the recent progress of measuring the cellular mechanical properties using AFM was summarized, and the challenges of AFM single-cell nanomechanical analysis were discussed.

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Section: Principle and Methods Of Afm Indentation Techniquesmentioning

confidence: 99%

Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy

Liu

et al. 2014

Chin. Sci. Bull.

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Section: Poc Nanobiosensors and Microbiome‐related Biomarkersmentioning

confidence: 99%

The Microbiome Meets Nanotechnology: Opportunities and Challenges in Developing New Diagnostic Devices

et al. 2021

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Monitoring of the human microbiome is an emerging area of diagnostics for personalized medicine. Here, the potential of different nanomaterials and nanobiosensing technologies is reviewed for the development of novel diagnostic devices for the detection and measurement of microbiome‐related biomarkers. Moreover, the current and future landscape of microbiome‐based diagnostics is defined by exploring the advantages and disadvantages of current nanotechnology‐based approaches, especially in the context of developing point‐of‐care (PoC) devices that would meet the international guidelines known as REASSURED (Real‐time connectivity; Ease of specimen collection; Affordability; Sensitivity; Specificity; User‐friendliness; Rapid & robust operation; Equipment‐free; and Deliverability). Finally, the strategies of the latest international scientific consortia working in this field are analyzed, the current microbiome diagnostics market are reported and the principal ethical, legal, and societal issues related to microbiome R&D and innovation are discussed.

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“…However, the exact origins of the fluctuations are still subject of scientific debate. This novel principle has so far only been demonstrated for two different bacteria phyla by one research group [15,26], but should theoretically be extendable to all organisms that exhibit detectable fluctuations [65], such as bacteria [64,68], fungi [69,70] and animals [66,71,72]. Therefore, it is a very promising tool for various biological monitoring applications.…”

Section: Contemporary Physics 191mentioning

confidence: 99%

“…Therefore, it is a very promising tool for various biological monitoring applications. Besides fundamental research on the corresponding biological samples, future applications may include rapid diagnostic, especially for infections with slow-growing bacteria species such as Mycobacterium tuberculosis (TB); as well as improvements in therapy as the antibacterial efficacy can be tested on cantilever sensors ahead of treatment, which preserves the effectiveness of antibiotics and curbs the development of bacterial resistance [26].…”

Section: Contemporary Physics 191mentioning

confidence: 99%

“…In contrast to the two aforementioned working principles, this principle does not require asymmetrical adsorption. The monitored fluctuations are believed to arise from various different metabolic processes within the living samples, which then get transduced onto the cantilever and consequently amplified [15,26]. However, the exact origins of the fluctuations are still subject of scientific debate.…”

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confidence: 99%

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Good vibrations for bad bacteria

Cited by 5 publications

References 9 publications

Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy

Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy

The Microbiome Meets Nanotechnology: Opportunities and Challenges in Developing New Diagnostic Devices

Cantilevers for biological monitoring: opportunities for classical and quantum physics

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