Goose parvovirus structural proteins expressed by recombinant baculoviruses self-assemble into virus-like particles with strong immunogenicity in goose

Development and evaluation of a competitive ELISA using a monoclonal antibody for antibody detection after goose parvovirus virus-like particles (VLPs) and vaccine immunization in goose sera

“…Serum samples (15 geese per group, seven groups, and 0-8th weeks) were collected (Ju et al, 2011). Specifically, 4-day-old geese from Datong Farm were housed in a specific pathogen-free facility at the NEAU Experimental Animal Center with free access to water and food ad libitum.…”

“…Then, the cells were incubated on ice for 30 min and centrifuged (12,000 × g, 10 min, 4 • C). The supernatant was stored at −20 • C (Ju et al, 2011).…”

“…The virus was derived from GPV 98E at the 15th-egg-passage, which resulted in a 100% duck embryo mortality rate (from the Laboratory Animal Center of Harbin Veterinary Research Institute, CAAS) after the 7th-egg-passage. rVP2-VLPs (Ju et al, 2011) were used as antigens for the MAbs screening by an indirect ELISA and were also the antigens that were used for the competitive ELISA. A total of 2 × 10 6 Sf9 cells in 4 × 100 cm 2 plastic flakes were infected with the recombinant baculoviruses VP2 at a MOI of 4.…”

“…Additionally, the 54 serum samples were validated with the neutralization assay (Ju et al, 2011) to calculate the sensitivity and the specificity of the C-ELISA.…”

“…The procedure of indirect ELISA is difficult to optimize because of sera's higher nonspecific adsorption (Sun, 2006). To overcome these limitations, a novel competitive ELISA has been developed that utilizes baculovirusexpressed recombinant VP2 virus-like particles (rVP2-VLPs) (Ju et al, 2011) and a newly developed monoclonal antibody against GPV.…”