Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis

Liang, Jinqian; Chen, Chong; Liu, Hongzhe; Li, Xiangyang; Li, Zheng; Hu, Jianhua; Zhao, Hong

doi:10.1155/2018/3635485

Cited by 8 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On one hand, this finding adds knowledge to the molecular mechanisms underlying gossypol biological function. Previously we reported the protective effects of gossypol on osteoporosis by enhancing Wnt signaling [9]. The current study further elucidated the inner link between gossypol and the Wnt signaling.…”

Section: Discussionsupporting

confidence: 71%

“…Some of the materials and methods were following the previous publication [9]. This study was approved by the Ethics Committee of Department of Orthopedic, People's Hospital of Hunan Province.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, we identified a new chemical, gossypol, to prevent osteoporosis by regulating cell proliferation and apoptosis [9]. Furthermore, Wnt signaling was demonstrated to be promoted by gossypol treatment in vivo and in vitro.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gossypol Promotes Wnt/β-Catenin Signaling through WIF1 in Ovariectomy-Induced Osteoporosis

Liang

Chen

Liu

et al. 2019

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Osteoporosis is one of the most frequent diseases related with age. Previously, we have reported a novel potential drug, gossypol, for the treatment of osteoporosis through its regulation of Wnt/β-catenin signaling. This study aims to identify the detailed mechanism of gossypol in human osteoporosis. Mice injected with gossypol were subjected for RNA-seq analysis and the transcription level of WIF1 was shown to be decreased dramatically in gossypol-treated mice, which was further confirmed by qRT-PCR and western blot analysis. Luciferase reporter assay showed gossypol inhibited the activity of WIF1 and the methylation of WIF1 was significantly upregulated, evidenced by ChIP assay. Cell viability assays demonstrated that gossypol promoted cell proliferation while cotreatment with WIF1 expressing plasmid reversed the effect in a dose- and time-dependent manner. Similarly, cell apoptotic assays and TUNEL assays showed gossypol suppressed cell apoptosis, which was revised by WIF1 overexpression. The mouse model suggested gossypol injection ameliorated osteoporosis, while coinjection of AAV5-WIF1 eliminated the protection effects of gossypol, as evidenced by H&E staining, serum osteocalcin level, serum OPG level, serum RANKL level, bone density, ultimate strength, and postyield displacement. This study is a supplement to the former publication, which reinforced the protection effect of gossypol in human osteoporosis.

show abstract

Section: Discussionsupporting

confidence: 71%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Gossypol Promotes Wnt/β-Catenin Signaling through WIF1 in Ovariectomy-Induced Osteoporosis

Liang

Chen

Liu

et al. 2019

BioMed Research International

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cells were stained with the propidium iodide (PI)/Annexin V-FITC kit (Sigma) in the dark for 10 min at room temperature [ 28 ]. Then, the cells were counted by flow cytometry (BD Biosciences).…”

Section: 6 5-ethynyl-2ʹ-deoxyuridine Staining (Edu)mentioning

confidence: 99%

“…TUNEL staining was used to assess BMSC death [ 28 ]. Briefly, BMSCs were fixed and treated with a TUNEL staining kit (Beyotime).…”

Section: 6 5-ethynyl-2ʹ-deoxyuridine Staining (Edu)mentioning

confidence: 99%

LncRNA MIAT can regulate the proliferation, apoptosis, and osteogenic differentiation of bone marrow-derived mesenchymal stem cells by targeting miR-150-5p

et al. 2022

View full text Add to dashboard Cite

Osteoporosis (OP) is a systemic bone metabolic disease with complicated pathogenesis and is difficult to cure clinically. The regulatory mechanisms of OP are needed to be further investigated. In the present study, we focused on the role of myocardial infarction-associated transcript (MIAT) in OP development and examined the underlying mechanism. The serum expression levels of MIAT in samples from patients with OP and healthy controls were compared using quantitative reverse transcription-PCR (qRT-PCR). The dual-luciferase reporter assay was used to confirm the relationship between MIAT and its potential target microRNA, i.e., miR-150-5p. Moreover, bone marrow-derived mesenchymal stem cells (BMSCs) were cultured and transfected with MIAT shRNA, with or without miR-150-5p inhibitor. EdU staining and colony formation analysis were performed to determine the proliferation ability of these cells. Furthermore, the TUNEL assay and flow cytometry were used to assess BMSC apoptosis. Finally, RT-PCR and Western blot assays were employed to assess the expression of osteogenic differentiation biomarkers. Compared with controls, the expression of MIAT was significantly increased, whereas that of miR-150-5p was markedly decreased in patients with OP. MIAT and miR-150-5p expression levels exhibited a strong negative correlation. Furthermore, osteogenic differentiation indicators were suppressed in serum of OP patients. MIAT was downregulated, and miR-150-5p was upregulated in induced to osteogenic differentiation BMSCs. Furthermore, downregulation of MIAT dramatically promoted osteogenic differentiation, increased proliferation, and inhibited apoptosis in BMSCs; miR-150-5p inhibitor abrogated the effects of MIAT. In conclusion, lncRNA MIAT can regulate the proliferation, apoptosis, and osteogenic differentiation of BMSCs.

show abstract

A critical assessment of the potential of pharmacological modulation of aldehyde dehydrogenases to treat the diseases of bone loss

Mittal

Bhagwati

Siddiqi

et al. 2020

European Journal of Pharmacology

View full text Add to dashboard Cite

Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis

Cited by 8 publications

References 24 publications

Gossypol Promotes Wnt/β-Catenin Signaling through WIF1 in Ovariectomy-Induced Osteoporosis

Gossypol Promotes Wnt/β-Catenin Signaling through WIF1 in Ovariectomy-Induced Osteoporosis

LncRNA MIAT can regulate the proliferation, apoptosis, and osteogenic differentiation of bone marrow-derived mesenchymal stem cells by targeting miR-150-5p

A critical assessment of the potential of pharmacological modulation of aldehyde dehydrogenases to treat the diseases of bone loss

Contact Info

Product

Resources

About