GPR39 marks specific cells within the sebaceous gland and contributes to skin wound healing

Zhao, Haitao; Qiao, Jingqiao; Zhang, Shoubing; Zhang, Huishan; Lei, Xiaohua; Wang, Xinyue; Deng, Zhili; Ning, Lina; Cao, Yuqi; Guo, Yong; Liu, Shuang; Duan, Enkui

doi:10.1038/srep07913

Cited by 26 publications

(19 citation statements)

References 35 publications

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“…Further investigations are required in order to determine whether the miniaturization of sebaceous glands in pemphigus is a feature of pemphigus or a result of androgenetic alopecia. It may be hypothesized that the miniaturization of sebaceous glands may lead to the impaired healing of pemphigus lesions on the scalp and, consequently, to the chronic and recalcitrant course of the disease in this location …”

Section: Discussionmentioning

confidence: 99%

Miniaturization of sebaceous glands: A novel histopathological finding in pemphigus vulgaris and pemphigus foliaceus of the scalp

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Miniaturization of sebaceous glands: A novel histopathological finding in pemphigus vulgaris and pemphigus foliaceus of the scalp

et al. 2017

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“…β-galactosidase (LacZ) staining were performed as previously described [ 36 ]. Briefly, mouse skin from the mid-dorsal region was harvested and fixed in 4% PFA in PBS for 30 min at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Exogenous R-Spondin1 Induces Precocious Telogen-to-Anagen Transition in Mouse Hair Follicles

Liu

Zhang

et al. 2016

IJMS

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R-spondin proteins are novel Wnt/β-catenin agonists, which signal through their receptors leucine-rich repeat-containing G-protein coupled receptor (LGR) 4/5/6 and substantially enhance Wnt/β-catenin activity. R-spondins are reported to function in embryonic development. They also play important roles in stem cell functions in adult tissues, such as the intestine and mammary glands, which largely rely on Wnt/β-catenin signaling. However, in the skin epithelium and hair follicles, the information about R-spondins is deficient, although the expressions and functions of their receptors, LGR4/5/6, have already been studied in detail. In the present study, highly-enriched expression of the R-spondin family genes (Rspo1/2/3/4) in the hair follicle dermal papilla is revealed. Expression of Rspo1 in the dermal papilla is specifically and prominently upregulated before anagen entry, and exogenous recombinant R-spondin1 protein injection in mid-telogen leads to precocious anagen entry. Moreover, R-spondin1 activates Wnt/β-catenin signaling in cultured bulge stem cells in vitro, changing their fate determination without altering the cell proliferation. Our pioneering study uncovers a role of R-spondin1 in the activation of cultured hair follicle stem cells and the regulation of hair cycle progression, shedding new light on the governance of Wnt/β-catenin signaling in skin biology and providing helpful clues for future treatment of hair follicle disorders.

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“…After the generation of cDNA, PCR was performed using Promega PCR Master Mix (Promega) as described previously [ 40 ]. The PCR procedure was as follows: 95 °C for 5 min; 25 or 30 cycles of 95 °C for 30 s, 56 °C for 30 s and 72 °C for 30 s and 72 °C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Spatiotemporal Expression of p63 in Mouse Epidermal Commitment

Zhao

Liu

Zhang

et al. 2015

IJMS

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The embryonic surface ectoderm is a simple flat epithelium consisting of cells that express the cytokeratins K8/K18. Before stratification, K5/K14 expression substitutes K8/K18 expression, marking the event called epidermal commitment. Previous studies show that the transcription factor p63 plays an essential role in epidermal commitment. However, detailed expression information of p63 during early epidermal development in mice is still unclear. We systematically studied the expression pattern of p63 in mouse epidermal commitment, together with K8 and K5. We show that p63 expression could be detected as early as E8.5 in mouse embryos preceding epidermal commitment. p63 expression first appears near the newly formed somites and the posterior part of the embryo, further expanding to the whole embryonic surface with particular enrichment in the first branchial arches and the limb buds. ΔNp63 is the major class of isoforms expressed in this period. Relative expression intensity of p63 depends on the embryonic position. In summary, there is a sequential and regular expression pattern of K8, p63 and K5 in mouse epidermal commitment. Our study not only contributes to understanding the early events during epidermal development but also provides a basal tool to study the function of p63 in mammals.

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GPR39 marks specific cells within the sebaceous gland and contributes to skin wound healing

Cited by 26 publications

References 35 publications

Miniaturization of sebaceous glands: A novel histopathological finding in pemphigus vulgaris and pemphigus foliaceus of the scalp

Miniaturization of sebaceous glands: A novel histopathological finding in pemphigus vulgaris and pemphigus foliaceus of the scalp

Exogenous R-Spondin1 Induces Precocious Telogen-to-Anagen Transition in Mouse Hair Follicles

Spatiotemporal Expression of p63 in Mouse Epidermal Commitment

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