GPx3 knockdown inhibits the proliferation and DNA synthesis and enhances the early apoptosis of human spermatogonial stem cells via mediating CXCL10 and cyclin B1

Cheng, Zhiguang; Ye, Peng; Chen, Ying; He, Zuping

doi:10.3389/fcell.2023.1213684

Cited by 4 publications

(1 citation statement)

References 41 publications

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“…In gastric cancer, the effect of two introns of the GPX3 gene, namely rs3805435 and rs3828599, on gene expression and disease susceptibility has been demonstrated (26). Furthermore, the expression of GPX3 rs736775 in patients undergoing adjuvant chemotherapy with platinum and fluorouracil has been associated with enhanced overall survival (45). Consequently, GPX3 rs736775 can be regarded as a potential prognostic marker.…”

Section: Gpx3mentioning

confidence: 99%

Advances in the role of GPX3 in ovarian cancer (Review)

Geng,

Zhou,

Wang

2024

Int J Oncol

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Ovarian cancer (OC) is the 5th most common malignancy in women, and the leading cause of death from gynecologic malignancies. Owing to tumor heterogeneity, lack of reliable early diagnostic methods and high incidence of chemotherapy resistance, the 5-year survival rate of patients with advanced OC remains low despite considerable advances in detection and therapeutic approaches. Therefore, identifying novel therapeutic targets to improve the prognosis of patients with OC is crucial. The expression of glutathione peroxidase 3 (GPX3) plays a crucial role in the growth, proliferation and differentiation of various malignant tumors. In OC, GPX3 is the only antioxidant enzyme the high expression of which is negatively correlated with the overall survival of patients. GPX3 may affect lipid metabolism in tumor stem cells by influencing redox homeostasis in the tumor microenvironment. The maintenance of stemness in OC stem cells (OCSCs) is strongly associated with poor prognosis and recurrence in patients. The aim of the present study was to review the role of GPX3 in OC and investigate the potential factors and effects of GPX3 on OCSCs. The findings of the current study offer novel potential targets for drug therapy in OC, enhance the theoretical foundation of OC drug therapy and provide valuable references for clinical treatment. Contents 1. Introduction 2. GPX3 3. OC and OCSCs 4. Association of GPX3 with OC 5. Conclusion and prospects

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Section: Gpx3mentioning

confidence: 99%

Advances in the role of GPX3 in ovarian cancer (Review)

Geng,

Zhou,

Wang

2024

Int J Oncol

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Integrative bioinformatics analysis to identify ferroptosis-related genes in non-obstructive azoospermia

Hong,

Yuan,

Wang

et al. 2024

J Assist Reprod Genet

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Single cell RNA sequencing reveals a shift in cell function and maturation of endogenous and infiltrating cell types in response to acute intervertebral disc injury

Clayton,

Sebastian,

Wilson

et al. 2024

Preprint

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Intervertebral disc (IVD) degeneration contributes to disabling back pain. Degeneration can be initiated by injury and progressively leads to irreversible cell loss and loss of IVD function. Attempts to restore IVD function through cell replacement therapies have had limited success due to knowledge gaps in critical cell populations and molecular crosstalk after injury. Here, we used single cell RNA sequencing to identify the transcriptional changes of endogenous and infiltrating IVD cell populations, as well as the potential of resident mesenchymal stem cells (MSCs) for tissue repair. Control and Injured (needle puncture) tail IVDs were extracted from 12 week old female C57BL/6 mice 7 days post injury and clustering analyses, gene ontology, and pseudotime trajectory analyses were used to determine transcriptomic divergences in the cells of the injured IVD, while immunofluorescence was utilized to determine mesenchymal stem cell (MSC) localization. Clustering analysis revealed 11 distinct cell populations that were IVD tissue specific, immune, or vascular cells. Differential gene expression analysis determined that Outer Annulus Fibrosus, Neutrophils, Saa2-High MSCs, Macrophages, and Krt18+Nucleus Pulposus (NP) cells were the major drivers of transcriptomic differences between Control and Injured cells. Gene ontology of DEGs suggested that the most upregulated biological pathways were angiogenesis and T cell related while wound healing and ECM regulation categories were downregulated. Pseudotime trajectory analyses revealed that cells were driven towards increased cell differentiation due to IVD injury in all IVD tissue clusters except for Krt18+NP which remained in a less mature cell state. Saa2-High and Grem1-High MSCs populations drifted towards more IVD differentiated cells profiles with injury and localized distinctly within the IVD. This study strengthens the understanding of heterogeneous IVD cell populations response to injury and identifies targetable MSC populations for future IVD repair studies.Lay SummaryThe intervertebral disc (IVD) is a spinal joint that accumulates damage with age but has limited tissue repair capabilities. IVD damage progresses into degeneration, and IVD degeneration is a leading cause of lower back pain. There are no effective therapies to treat IVD degeneration, but understanding the cell populations that change and respond to injury will uncover targets to restore IVD function. Mesenchymal stem cells (MSCs) are cells within the IVD that can potentially replenish the cells lost after IVD damage. To identify the cell populations of the IVD and how they change with injury, we performed single cell RNA sequencing of IVD tissue 7 days post injury and analyzed the differences in gene regulation. We identified diverse cells populations such as IVD specific tissues, immune cells, vascular cells, and MSCs. We discovered the presence ofSaa2andGrem1expressing MSCs that become less stem cell-like and express higher levels of IVD gene markers after injury. We also determined thatSaa2andGrem1have slightly different expression patterns in IVD tissues, and this expression becomes reduced after injury. These MSCs could be used in future stem cell therapies to prevent IVD degeneration.Abstract FigureGraphical AbstractIsolation of mouse coccygeal intervertebral discs for scRNASeq analyses.

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GPx3 knockdown inhibits the proliferation and DNA synthesis and enhances the early apoptosis of human spermatogonial stem cells via mediating CXCL10 and cyclin B1

Cited by 4 publications

References 41 publications

Advances in the role of GPX3 in ovarian cancer (Review)

Advances in the role of GPX3 in ovarian cancer (Review)

Integrative bioinformatics analysis to identify ferroptosis-related genes in non-obstructive azoospermia

Single cell RNA sequencing reveals a shift in cell function and maturation of endogenous and infiltrating cell types in response to acute intervertebral disc injury

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