Gradient Lateral Flow Immunoassay of Human Chorionic Gonadotropin

Serebrennikova, Kseniya V.; Samsonova, J.V.; Осипов, А. И.

doi:10.3103/s0027131418010066

Cited by 7 publications

(1 citation statement)

References 5 publications

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“…For assays requiring quantitative information, it is necessary to design the reaction response so that the visual off/on threshold value corresponds to the specific analyte concentration to be detected. Varying the concentration of capture reagent solutions deposited at a test line is one of the common strategies to control the threshold setting in LFIAs. ,− In the current approach, where test strips are read by the naked eye, text symbols at individual test zones are interpreted as either being readable or nonreadable. We aimed at controlling the threshold analyte concentration for text symbol appearance by making use of the feature of inkjet printing, enabling precise and flexible adjustment of deposited amounts of mask and text reagents at individual test zones by simply altering the number of printing cycles.…”

Section: Resultsmentioning

confidence: 99%

Text-Displaying Semiquantitative Competitive Lateral Flow Immunoassay Relying on Inkjet-Printed Patterns

Misawa

Yamamoto²,

Hiruta

et al. 2020

ACS Sens.

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This work describes a colorimetric signaling approach for competitive lateral flow immunoassays (LFIAs) enabling sensitive and semiquantitative direct visual result readout in the form of "text", demonstrated on the example of 8-hydroxy-2′-deoxyguanosine (8-OHdG) detection. The distinctive feature of the developed text-displaying LFIA (TD-LFIA) is the test zone system consisting of a combination of two types of inkjet-deposited capture molecules referred to as "mask antigen" and "text antibody", allowing for sensitive turn-on signaling as opposed to the inverse response of conventional competitive LFIAs. The user operation is limited to sample application, followed by direct reading of assay results written in text after approximately 10 min. TD-LFIAs enabled the visual detection of 8-OHdG at concentrations down to 3 ng/mL, which is a 2−3 orders of magnitude lower visual detection limit than that achieved with the corresponding conventional design and is comparable to the existing LFIAs relying on external signal readout equipment. Highly reproducible observer-independent assay performance was confirmed, and the result interpretation is not influenced by sample color and readout timing. Making use of customizable threshold settings for text appearance, a device for semiquantitative assays was developed and successfully applied to the detection of 8-OHdG at four concentration levels (trace, low, medium, and high) in 54 human urine samples within the clinically relevant concentration range. The sensitive and intuitive signaling method of the developed system offers great potential for an alternative competitive LFIA platform suitable for real-world point-of-care testing applications.

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