Gradual increase in priming of human eosinophils during extravasation from peripheral blood to the airways in response to allergen challenge

Luijk, Bart; Lindemans, Caroline A.; Kanters, Deon; Heijde, Roos van der; Bertics, Paul J.; Lammers, Jan‐Willem J.; Bates, Mary Ellen; Koenderman, Leo

doi:10.1016/j.jaci.2005.02.002

Cited by 50 publications

(64 citation statements)

References 29 publications

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“…Finally, we found that in the presence of LTD 4 , VCAM-1 induces the release of EDN. These results suggest a mechanism to explain previous reports that eosinophils are functionally upregulated in the airway [17,18,19,20], and suggest that adhesion to and migration across endothelial cells via interaction with specific adhesion proteins and then exposure to cysLTs may be essential processes underlying activation of the effector functions of eosinophils in the asthmatic airway.…”

Section: Discussionsupporting

confidence: 74%

Eosinophil Superoxide Anion Generation Induced by Adhesion Molecules and Leukotriene D<sub>4</sub>

Mori

Takaku

Kobayashi

et al. 2009

Int Arch Allergy Immunol

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Rationale: Eosinophils preferentially accumulate at sites of inflammation in the asthmatic airway. Participation of circulating eosinophils in the airway inflammation in asthma involves their interaction with adhesion molecules expressed on the endothelial cell surface and exposure to inflammatory mediators, such as cysteinyl leukotrienes (cysLTs). Objective: To investigate whether interaction of eosinophils with adhesion molecules modifies the functions of these cells induced by cysLTs. Methods: Eosinophils were isolated from the blood of healthy donors, incubated in the EIA plates coated with adhesion proteins, and then exposed to LTD₄. The generation of superoxide anion (O₂^–), adhesion to the plates, and release of eosinophil-derived neutrotoxin (EDN) were evaluated. Results: Neither VCAM-1 nor LTD₄ (100 nM) independently induced eosinophil O₂^– generation, however, combined exposure to the two molecules synergistically induced eosinophil O₂^– generation. ICAM-1 by itself induced eosinophil O₂^– generation, which was enhanced by LTD₄. On the contrary, P-selectin did not induce O₂^– generation, either in the presence or absence of LTD₄. LTD₄ significantly enhanced eosinophil adhesion to rh-VCAM-1 and rh-ICAM-1, but not to rh-P-selectin. Finally, we observed that combined exposure of eosinophils to LTD₄ and VCAM-1 induced the release of EDN. Conclusion: Combined exposure to VCAM-1 or ICAM-1 and cysLT effectively induces the effector functions of eosinophils. Eosinophil adhesion to and migration across endothelial cells via these specific adhesion proteins and subsequent exposure to cysLTs may be mechanisms underlying activation of the effector functions of eosinophils in the asthmatic airway.

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Section: Discussionsupporting

confidence: 74%

Eosinophil Superoxide Anion Generation Induced by Adhesion Molecules and Leukotriene D<sub>4</sub>

Mori

Takaku

Kobayashi

et al. 2009

Int Arch Allergy Immunol

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“…14,15 Priming of these inflammatory cells is particularly associated with an enhanced functionality of adhesion-associated responses that facilitates the recruitment of eosinophils from the blood to the airways. This was shown by increased chemotactic responses and transendothelial movement of these cells, which were in part mediated by upregulation of adhesion molecules.…”

mentioning

confidence: 99%

Expression of activated FcγRII discriminates between multiple granulocyte-priming phenotypes in peripheral blood of allergic asthmatic subjects

Kanters

Hove

Luijk

et al. 2007

Journal of Allergy and Clinical Immunology

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“…This highlights the complexity of the mechanisms involved in the regulation of the number of eosinophils present in the airways. In this regard it has recently been suggested that priming of circulating eosinophils is a critical step prior to cell extravasation and tissular recruitment (18).…”

Section: Discussionmentioning

confidence: 99%

Cysteinyl‐leukotrienes contribute to sputum eosinophil chemotactic activity in asthmatics

Hemelaers

Henket

Sele

et al. 2005

Allergy

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Background: Cysteinyl-leukotrienes are lipid derived mediators involved in asthma. They are able to stimulate eosinophil chemotaxis in vitro. Induced sputum from asthmatics has been shown to contain eosinophil chemotactic activity. The purpose of our study was to evaluate the contribution of cysteinyl-leukotrienes to sputum eosinophil chemotactic activity in asthmatics and to seek whether there might be differences between asthmatics free of inhaled corticosteroids vs those regularly receiving this treatment. Methods: Twenty-two patients (11 corticosteroid free, mean FEV 1 99% predicted, 11 corticosteroid-treated, mean FEV 1 77% predicted) recruited from our asthma clinic underwent a sputum induction. Sputum was processed according to standard procedure. Eosinophil chemotactic activity contained in the fluid phase was assessed using Boyden microchamber model and expressed as chemotaxis index (CI). Cysteinyl-leukotrienes were measured in sputum supernatant by ELISA and their role in sputum eosionophil chemotactic activity was evaluated by using montelukast, a selective antagonist of a cys-LT1 receptor. Results: Cysteinyl-leukotrienes were well detectable in sputum supernatants from both steroid-naive (247 ± 42 pg/ml) and steroid-treated (228 ± 26 pg/ml) asthmatics. Sputum eosinophil chemotactic activity was indiscriminately present in both corticosteroid-naive (CI: 2.61 ± 0.22) and corticosteroid-treated (2.98 ± 0.35) asthmatics. Montelukast (100 µM) significantly inhibited the eosinophil chemotactic activity in both groups achieving a mean inhibition of 54.2 ± 9.2% (P < 0.001) and 64.7 ± 7.8% (P < 0.001) in steroid-naive and steroid-treated asthmatics respectively. Conclusion: Cysteinyl-leukotrienes actively participate in sputum eosinophil chemotactic activity found in asthmatics irrespective of whether they are or not under treatment with inhaled corticoids.

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Gradual increase in priming of human eosinophils during extravasation from peripheral blood to the airways in response to allergen challenge

Cited by 50 publications

References 29 publications

Eosinophil Superoxide Anion Generation Induced by Adhesion Molecules and Leukotriene D<sub>4</sub>

Eosinophil Superoxide Anion Generation Induced by Adhesion Molecules and Leukotriene D<sub>4</sub>

Expression of activated FcγRII discriminates between multiple granulocyte-priming phenotypes in peripheral blood of allergic asthmatic subjects

Cysteinyl‐leukotrienes contribute to sputum eosinophil chemotactic activity in asthmatics

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