Gradually scale-up culture in a bioreactor promotes radical scavenging activity of Panax ginseng (C. A. Meyer) adventitious roots on 1,1-diphenyl-2-picrylhydrazyl

Abstract

A scale-up culture of adventitious roots of ginseng was established using a balloon-type bubble bioreactor (BTBB). Maximum growth rates of *52-fold and *50-fold in 3 and 5 L BTBBs were obtained, respectively after 40 days of inoculation, which was significantly higher than that in 0.5 L conical flask (*15-fold). Gradually scale-up culture of adventitious roots increased the root biomass, while the contents of ginsenoside and polysaccharides were not affected. This study also revealed that radical scavenging activity of dried adventitious roots on 1,1-diphenyl-2-picrylhydrazyl was higher than that of native roots at 20-100 mg L -1 methanolic extract.

Use of partial least-squares discriminant analysis to study the effects of gradual scale-up of culture, and optimization of bioreactor angle and aeration volume on culture of Panax quinquefolium L. adventitious roots in a 5-L balloon-type bubble bioreactor

“…Bioreactor culture has many advantages Gradual scale-up of culture, optimization of bioreactor angle over conical flask culture because culture conditions in bioreactors can be optimized by real-time manipulation of temperature, pH, rate of aeration, and levels of carbon dioxide and nutrients in the medium. Wang et al [20] found that the rate of growth of ginseng adventitious roots reached 49.84-fold after 40 days of culture in a 5-L BTBB. Choi et al [21] reported that the maximum growth rate of ginseng adventitious roots in 5 and 20-L bioreactors was 7.33-fold and 8.17-fold, respectively, after 42 days.…”