Graduated Embryo Score and soluble human leukocyte antigen-G expression improve assisted reproductive technology outcomes and suggest a basis for elective single-embryo transfer

Fisch, Jeffrey D; Keskintepe, Levent; Ginsburg, Michael; Adamowicz, Mark; Sher, Geoffrey

doi:10.1016/j.fertnstert.2006.08.088

Cited by 38 publications

(17 citation statements)

References 24 publications

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“…In the study presented here, a traditional Day 3 morphology compared to the sHLA-G expression plus a GES-score in order to identify embryos for transfer resulting into favorable clinical and ongoing pregnancy rates. These findings are supported by a number of researchers [20,[23][24][25][26]. Furthermore, we reported a decrease of fetal loss, in the sHLA-G positive group, between 6 week and 12 week ultrasound, a finding supported by Rebmann et al [27].…”

Section: Treatment Groups Percent Of Fetusses Lost (%)supporting

confidence: 88%

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Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome

Kotzé

Hansen

Keskintepe

et al. 2010

J Assist Reprod Genet

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Section: Treatment Groups Percent Of Fetusses Lost (%)supporting

confidence: 88%

“…This original optimal range was determined by Keskintepe (unpublished data) and was slightly different from other investigators [20,24].…”

Section: Introductionmentioning

confidence: 87%

Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome

Kotzé

Hansen

Keskintepe

et al. 2010

J Assist Reprod Genet

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“…Due to the low sHLA-G concentration in embryo cultures (EC), the HLA-G immunodetection method and sample treatment strongly determine the reliability of the sHLA-G measurement in embryo culture supernatants. Technical difficulties have led to very controversial results in the past [100,[104][105][106][107][108][109][110][111][112][113][114][115][116][117][118] but are being resolved. sHLA-G in embryo culture supernatants is mostly measured by sandwich ELISA or ALBIA (addressable laser bead immunoassay) techniques.…”

Section: Hla-g Expression In Preimplantation Embryos and Its Clinicalmentioning

confidence: 99%

The importance of HLA-G expression in embryos, trophoblast cells, and embryonic stem cells

Rizzo

Vercammen

Velde

et al. 2010

Cell. Mol. Life Sci.

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The nonclassical HLA-G molecule is a trophoblast-specific molecule present in almost every pregnancy. It differs from classical HLA class I molecules by the low degree of allelic variants and the high diversity of protein structures. HLA-G is reported to be a tolerogenic molecule that acts on cells of both innate and adaptive immunity. At the maternal-fetal interface HLA-G seems to be responsible largely for the reprogramming of local maternal immune response. This review will focus on the HLA-G gene expression profile in pregnancy, in preimplantation embryos, and in human embryonic stem cells with emphasis on the structural diversity of the HLA-G protein and its potential functional and diagnostic implications.

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“…During recent years, several studies suggested a relationship between the production of sHLA-G molecules by early embryos and an increased implantation rate in IVF protocols (24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)12). Two studies (36,37) failed to detect sHLA-G molecules in embryo culture supernatants, probably due to technical discrepancies, as suggested by the recent review by Warner et al (38).…”

Section: Discussionmentioning

confidence: 99%

Production of sHLA-G molecules by in vitro matured cumulus-oocyte complex

Rizzo

Canto²,

Stignani³

et al. 2009

Int J Mol Med

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Abstract. Oocyte selection with the highest competence is a major goal in IVF. Several studies demonstrated that nonclassical HLA class I HLA-G molecule modulation creates a tolerogenic microenvironment at the feto-maternal interface and is implicated in embryo implantation. This study investigated if soluble HLA-G molecules producted by the cumulus-oocyte complex (COC) are markers of oocyte maturation. sHLA-G molecule levels were analyzed using Bio-Plex assay in 152 COC supernatants obtained from 42 women and maturated by an 'in vitro maturation procedure'. The presence of sHLA-G molecules was confirmed by Western blotting technique. The results demonstrate detectable amounts of sHLA-G molecules ranging from 300 to 800 pg/ml in 14/73 (19%) COCs that generated mature oocytes and complete absence of detectable sHLA-G antigens in the supernatants of COCs that corresponded to immature oocytes. The detection of sHLA-G molecules in the COC culture supernatants corresponding to matured oocytes is proposed to be a marker to identify gametes with higher functionality. This non-invasive marker could be used, in addition to morphological approaches, to reduce the number of fertilized oocytes and transferred embryos.

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Graduated Embryo Score and soluble human leukocyte antigen-G expression improve assisted reproductive technology outcomes and suggest a basis for elective single-embryo transfer

Cited by 38 publications

References 24 publications

Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome

Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome

The importance of HLA-G expression in embryos, trophoblast cells, and embryonic stem cells

Production of sHLA-G molecules by in vitro matured cumulus-oocyte complex

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