Grape seed proanthocyanidin extract protects human umbilical vein endothelial cells from indoxyl sulfate-induced injury via ameliorating mitochondrial dysfunction

Lu, Zhaoyu; Lu, Fuhua; Zheng, Yingxia; Zeng, Yuqun; Zou, Chuan; Liu, Xusheng

doi:10.3109/0886022x.2015.1104609

Cited by 20 publications

(14 citation statements)

References 32 publications

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“…Therefore, we found that OPC can inhibit apoptosis by protecting mitochondria. Similar results have been proven in other kinds of cells (39)(40)(41)(42)(43). In this study, we used chondrocytes derived from mice and did not compare with chondrocytes of other animals.…”

Section: Discussionsupporting

confidence: 74%

Oligomeric proanthocyanidins inhibit apoptosis of chondrocytes induced by interleukin-1β

Yin

Wang

et al. 2017

Molecular Medicine Reports

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Oligomeric proanthocyanidin (OPC) is a water-soluble plant polyphenolic compound known for its cytoprotective effects in various tissue types. However, its effect on chondrocytes has not been well characterized. The present study aimed to investigate the effect of OPC on interleukin-1β (IL-1β)-induced apoptosis in chondrocytes, and to determine the mechanisms underlying the protective effects of OPC. Knee articular chondrocytes obtained from 6-week-old SPF Kunming mice were cultured and serially passaged. First-generation chondrocytes were selected for subsequent experiments following toluidine blue staining. Subsequent to IL-1β and OPC administration, an MTT assay was performed to examine the viability rate of chondrocytes, and the optimal drug concentration was determined. The fluorescence dye 2',7'-dichlorofluorescein diacetate was used to determine the intracellular content of reactive oxygen species (ROS). Mitochondrial membrane potential (MMP) was measured using a 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolylcarbocyanine iodide (JC-1) assay. The apoptosis rate of chondrocytes was assessed using an Annexin V-FITC/PI assay and ultrastructural changes were observed under an electron microscope. The results demonstrated that OPC increased the survival rate of chondrocytes against IL-1β-induced apoptosis. The most significant protective effect of OPC was observed at the concentration of 0.050 mg/ml. OPC reversed the increased ROS content and MMP levels, and inhibited IL-1β-induced apoptosis in chondrocytes. In addition, OPC was revealed to protect the ultrastructural integrity of chondrocytes. Taken together, the results of the present study suggest that OPC protects chondrocytes against IL-1β-induced damage by decreasing ROS content and MMP levels.

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Section: Discussionsupporting

confidence: 74%

Oligomeric proanthocyanidins inhibit apoptosis of chondrocytes induced by interleukin-1β

Yin

Wang

et al. 2017

Molecular Medicine Reports

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“…Açaí fruits from the Amazon region (Belém do Pará, Brazil, 2 • 57 ′ 29,47 ″ S/47 • 23 ′ 10,37 ″ W) were processed to obtain the hydro-alcoholic extract as previously described (Rocha et al 2007 HUVECs preserve endothelial cell native characteristics, including the expression of specific surface markers and intracellular signalling pathways. HUVECs were chosen as an experimental model in this study, since they have been a useful model to investigate endothelial dysfunction promoted by uraemic toxins (Dou et al 2004(Dou et al , 2007Faure et al 2006;Gross et al 2015;Lu et al 2016;Migliori et al 2015) and also other biological actions (Addi et al 2019;Shen et al 2016).…”

Section: Açaí Seed Extractmentioning

confidence: 99%

Uraemic toxin‐induced inflammation and oxidative stress in human endothelial cells: protective effect of polyphenol‐rich extract from açaí

Monteiro

Soares

Trindade

et al. 2020

Experimental Physiology

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New Findings What is the central question of this study?Does a polyphenol‐rich extract from açaí have a potential role in preventing uraemic toxin‐induced endothelial cell dysfunction? What is the main finding and its importance?Polyphenols from açaí prevented cell death, restored migratory capacity, protected from inflammation and contributed to the restoration of the antioxidant response in endothelial cells exposed to uraemic toxins. The protective role of açaí against toxic effects exerted by uraemic toxins presents a potential new therapeutic target in endothelial cells. Abstract In chronic kidney disease (CKD), progressive loss of kidney function results in the accumulation of protein‐bound uraemic toxins such as p‐cresyl sulfate (pCS) and indoxyl sulfate (IS). Among strategies to ameliorate the harmful actions of uraemic toxins, phenolic compounds have been extensively studied. The main goal of this work was to evaluate the antioxidant and anti‐inflammatory actions of phenolic‐rich açaí seed extract (ASE) in response to endothelial dysfunction induced by IS and pCS, in human umbilical vein endothelial cells (HUVECs). Cells were treated with ASE (10 µg ml−1) in the presence or absence of IS (61 µg ml−1) and pCS (40 µg ml−1). Cell viability, cell death, cell migratory capacity and inflammatory biomarker expression were evaluated. Cellular antioxidant response was measured through the activity and expression of antioxidant enzymes, and oxidative damage was evaluated. IS and pCS lowered cell viability, triggered cell death and lowered the migratory capacity in endothelial cells (P < 0.05). ASE prevented cell death and restored the migratory capacity in cells exposed to IS. Both toxins up‐regulated pro‐inflammatory cytokine expression, and ASE was able to beneficially counteract this effect. Tumour necrosis factor‐α secretion was greater in uraemic toxin‐treated cells and ASE reversed this phenomenon in cells treated with both toxins concomitantly (P < 0.05). With regard to the antioxidant response, superoxide dismutase expression was strikingly lower in cells treated with both toxins, and ASE inhibited this harmful effect (P < 0.05). From the results, we conclude that ASE exerted protective effects on inflammation and oxidative stress caused by uraemic toxins (particularly by IS) in human endothelial cells.

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“…Proanthocyanidin, a class of polyphenols with strong antioxidant properties commonly found in a variety of plants, have been reported to exert cell protective effects by reducing mitochondria damage and inhibiting cell apoptosis (Gao et al, 2014; Lu et al, 2015; Zhen et al, 2014). Grape seed proanthocyanidin extract (GSPE), an extract from grape seed, contains phenolic hydroxyl groups, which are well-known antioxidants (Ding et al, 2013; Mansouri et al, 2011), safe and well tolerated in humans (Sano, in press).…”

Section: Introductionmentioning

confidence: 99%

Grape seed proanthocyanidin extract protects lymphocytes against histone-induced apoptosis

Chang

Cauvi

et al. 2017

PeerJ

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Apoptosis of lymphocytes is associated with immunosuppression and poor prognosis in sepsis. Our previous report showed that histones, nuclear proteins released from damaged or dying cells in sepsis, can mediate lymphocyte apoptosis via mitochondria damage. Grape seed proanthocyanidin extract (GSPE), a natural substance with protective properties against oxidative stress, plays a vital role in cell and mitochondria protection. We thus hypothesized that GSPE may play a protective role in histone-induced lymphocyte apoptosis through its anti-oxidative properties. In this study, we investigated the protective efficacy of GSPE on lymphocyte apoptosis induced by extracellular histones, a main contributor of death in sepsis. Human blood lymphocytes were treated with 50 μg/ml histones, 2 μg/ml GSPE, or a combination of both. A total of 100 μM N-acetylcysteine (NAC), a reactive oxygen species (ROS) inhibitor, was used as a positive control for GSPE. Apoptosis, intracellular ROS levels, mitochondrial membrane potential, Bcl-2 expression, and caspase-3 cleavage were measured. Our data clearly indicate that GSPE significantly inhibited lymphocyte apoptosis, generation of ROS, the loss of mitochondrial membrane potential, the decrease in Bcl-2 expression, and caspase-3 activation induced by extracellular histones. In conclusion, we show that GSPE has a protective effect on lymphocyte apoptosis induced by extracellular histones. This study suggests GSPE as a potential therapeutic agent that could help reduce lymphocyte apoptosis, and thus the state of immunosuppression was observed in septic patients.

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Grape seed proanthocyanidin extract protects human umbilical vein endothelial cells from indoxyl sulfate-induced injury via ameliorating mitochondrial dysfunction

Cited by 20 publications

References 32 publications

Oligomeric proanthocyanidins inhibit apoptosis of chondrocytes induced by interleukin-1β

Oligomeric proanthocyanidins inhibit apoptosis of chondrocytes induced by interleukin-1β

Uraemic toxin‐induced inflammation and oxidative stress in human endothelial cells: protective effect of polyphenol‐rich extract from açaí

Grape seed proanthocyanidin extract protects lymphocytes against histone-induced apoptosis

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