Grapefruit-derived extracellular vesicles as a promising cell-free therapeutic tool for wound healing

Abstract

Due to the prevalence of individuals suffering from chronic wounds, developing safe and effective wound care agents is one of the more prominent fields of research in biology. However, wound...

Emergence of Edible Plant-Derived Nanovesicles as Functional Food Components and Nanocarriers for Therapeutics Delivery: Potentials in Human Health and Disease

“…Pre-processing steps for PDNVs are largely the same with a slight modification depending on the type of fruits or the structure of vegetables. For example, fleshy fruits, such as apple [ 18 , 19 ], blueberry [ 20 , 21 ], orange [ 22 , 23 ], lemon [ 24 ], and grapefruit [ 25 , 26 ], having high water content, were crushed/smashed and then homogenized using a blender or squeezed manually or pressed using a juicer. The collected juice was then processed to isolate nanoparticles.…”

“…In terms of quantity, approximately 1 mg of orange-derived nanovesicle protein is obtained in 350 mL juice [ 22 ]. Presence of the heat shock protein HSP70 or tetraspanins, such as CD9 and CD63, which are generally accepted as exosome markers, were confirmed in PDNVs [ 26 , 32 ]. When the Citrus -derived nanovesicles protein dataset was compared with the ExoCarta database, 56.7% proteins overlapped with mammalian exosome proteins [ 24 ].…”

Preclinical Studies on Convalescent Human Immune Plasma-Derived Exosome: Omics and Antiviral Properties to SARS-CoV-2

“…After the centrifugation, the top layer was removed, and 1 ml of the sucrose layer was collected from the bottom carefully to ensure the exosome-containing phase remained unmixed with the contaminants of the upper phase. ATPS isolation of convalescent human immune plasma-derived exosomes (ChipEXOs) was performed as previously described ( 23 , 29 ). Briefly, samples were mixed at a 1:1 (v/v) ratio with the isolation solution, which consists of PEG and dextran.…”

Oral administration of sea cucumber (Stichopus japonicus) protein exerts wound healing effectsviathe PI3K/AKT/mTOR signaling pathway

“…The wound proliferation involves granulation tissue formation and re-epithelialization, and was characterized by newly formed epithelium, blood vessels, and the deposition of collagen. 27 Among them, the formation of granulation tissue included collagen synthesis and neovascularization of endothelial cells. Re-epithelialization was achieved through cell migration of keratinocytes.…”