Grapevine crown gall caused by Rhizobium radiobacter (Ti) in Japan

Kawaguchi, Akira; Inoue, Koji

doi:10.1007/s10327-009-0156-2

Cited by 9 publications

(3 citation statements)

References 23 publications

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“…We previously reported that the nonpathogenic R. vitis strain, VAR03-1, which was isolated from a grapevine nursery stock in Japan, inhibited tumor formation caused by several R. radiobacter (Ti), R. rhizogenes (Ti), and R. vitis (Ti) strains isolated from different plants in Japan on grapevines, roses, tomatoes, sunflowers, and apples (18, 19, 21, 22, 23, 25). Repetitive sequence-based polymerase chain reaction (rep-PCR) DNA fingerprint analysis and an analysis of the sequences of three housekeeping genes revealed that VAR03-1 differed genetically from the tumorigenic and non-antagonistic R. vitis strains (21, 26).…”

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Reduction in Pathogen Populations at Grapevine Wound Sites is Associated with the Mechanism Underlying the Biological Control of Crown Gall by <i>Rhizobium vitis</i> Strain ARK-1

Kawaguchi¹

2014

Microb. Environ.

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A nonpathogenic strain of Rhizobium (=Agrobacterium) vitis, ARK-1, limited the development of grapevine crown gall. A co-inoculation with ARK-1 and the tumorigenic strain VAT07-1 at a 1:1 cell ratio resulted in a higher population of ARK-1 than VAT07-1 in shoots without tumors, but a significantly lower population of ARK-1 than VAT07-1 in grapevine shoots with tumors. ARK-1 began to significantly suppress the VAT07-1 population 2 d after the inoculation. This result indicated that ARK-1 reduced the pathogen population at the wound site through biological control. Although ARK-1 produced a zone of inhibition against other tumorigenic Rhizobium spp. in in vitro assays, antibiosis depended on the culture medium. ARK-1 did not inhibit the growth of tumorigenic R. radiobacter strain AtC1 in the antibiosis assay, but suppressed the AtC1-induced formation of tumors on grapevine shoots, suggesting that antibiosis by ARK-1 may not be the main mechanism responsible for biological control.

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Reduction in Pathogen Populations at Grapevine Wound Sites is Associated with the Mechanism Underlying the Biological Control of Crown Gall by <i>Rhizobium vitis</i> Strain ARK-1

Kawaguchi¹

2014

Microb. Environ.

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“…Rhizobium vitis (Ti), Agrobacterium vitis (Ti), and A. tumefaciens biovar 3), where Ti means tumorigenic [ 1 , 2 ]. A. vitis (Ti) infects grapevines through wounds, such as freezing injuries, cutting damage, and grafting [ 3 , 4 , 5 , 6 , 7 , 8 ]. GCG is a pandemic throughout the world [ 3 , 4 , 5 , 6 ].…”

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“…GCG is a pandemic throughout the world [ 3 , 4 , 5 , 6 ]. Galls generally form on the trunks and cordons of young and mature grapevines [ 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. Infected grapevines often experience inferior growth, but the galls cause grapevine death [ 7 ].…”

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Biological Control for Grapevine Crown Gall Evaluated by a Network Meta-Analysis

Kawaguchi

Kirino²,

Inoue³

2023

Plants

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Grapevine crown gall (GCG), which is caused by Allorhizobium vitis (=Rhizobium vitis) tumorigenic strains, is the most important disease of grapevine around the world. Previously, nonpathogenic A. vitis strains VAR03-1, ARK-1, ARK-2, and ARK-3 were identified as promising biological control agents, but the control effects of each strain were not directly compared and assessed in the field because field trials were conducted in different fields and years. Thus, the results of the control effects obtained from 16 field trials in 12 years from 2006 to 2017 were analyzed and evaluated by a linear mixed model (LMM) and a network meta-analysis (NMA). The results of the LMM strongly indicate that the factor “antagonistic strain” was significantly related to the biological control activity in this study, but the other factors, “concentration of cell suspension”, “field”, and “year”, were not. Then, the results of 16 field trials were combined in an NMA. The estimated relative risk (RR) after treatment with ARK-1, ARK-2, ARK-3, VAR03-1, and K84 were 0.16, 0.20, 0.22, 0.24, and 0.74, respectively. In conclusion, strain ARK-1 was the best antagonist regardless of the concentration of the cell suspension, field, and year differences, and it can be recommended to control GCG.

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The Ecology of Agrobacterium vitis and Management of Crown Gall Disease in Vineyards

Kuzmanović

Puławska

Hao

et al. 2018

Current Topics in Microbiology and Immunology

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Agrobacterium vitis is the primary causal agent of grapevine crown gall worldwide. Symptoms of grapevine crown gall disease include tumor formation on the aerial plant parts, whereas both tumorigenic and nontumorigenic strains of A. vitis cause root necrosis. Genetic and genomic analyses indicated that A. vitis is distinguishable from the members of the Agrobacterium genus and its transfer to the genus Allorhizobium was suggested. A. vitis is genetically diverse, with respect to both chromosomal and plasmid DNA. Its pathogenicity is mainly determined by a large conjugal tumor-inducing (Ti) plasmid characterized by a mosaic structure with conserved and variable regions. Traditionally, A. vitis Ti plasmids and host strains were differentiated into octopine/cucumopine, nopaline, and vitopine groups, based on opine markers. However, tumorigenic and nontumorigenic strains of A. vitis may carry other ecologically important plasmids, such as tartrate- and opine-catabolic plasmids. A. vitis colonizes vines endophytically. It is also able to survive epiphytically on grapevine plants and is detected in soil exclusively in association with grapevine plants. Because A. vitis persists systemically in symptomless grapevine plants, it can be efficiently disseminated to distant geographical areas via international trade of propagation material. The use of healthy planting material in areas with no history of the crown gall represents the crucial measure of disease management. Moreover, biological control and production of resistant grape varieties are encouraging as future control measures.

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Grapevine crown gall caused by Rhizobium radiobacter (Ti) in Japan

Cited by 9 publications

References 23 publications

Reduction in Pathogen Populations at Grapevine Wound Sites is Associated with the Mechanism Underlying the Biological Control of Crown Gall by <i>Rhizobium vitis</i> Strain ARK-1

Reduction in Pathogen Populations at Grapevine Wound Sites is Associated with the Mechanism Underlying the Biological Control of Crown Gall by <i>Rhizobium vitis</i> Strain ARK-1

Biological Control for Grapevine Crown Gall Evaluated by a Network Meta-Analysis

The Ecology of Agrobacterium vitis and Management of Crown Gall Disease in Vineyards

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