Grapevine microRNAs responsive to exogenous gibberellin

jinsoo, Han; Fang, Jinggui; Wang, Chen; Yi, Yin; Sun, Xiaowen; Leng, Xiangpeng; Song, Chuyi

doi:10.1186/1471-2164-15-111

Cited by 51 publications

(56 citation statements)

References 39 publications

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“…Exogenous GA application and its role in viticulture have been studied by many researchers [153][154][155][156][157][158][159][160][161]. The exogenous pre-bloom application of gibberellic acid (GA 3 ) to grape cultivars, that is normally used to establish early ripening [153], also induced seedlessness [154,158], and enhanced berry size in seedless cultivars [155][156][157].…”

Section: Gibberellinsmentioning

confidence: 99%

“…The exogenous pre-bloom application of gibberellic acid (GA 3 ) to grape cultivars, that is normally used to establish early ripening [153], also induced seedlessness [154,158], and enhanced berry size in seedless cultivars [155][156][157]. Transcriptome sequencing conducted to identify microRNAs during GA 3 application suggests that grapevine miRNAs (Vvi-miRNAs) might have a role in grape berry development and response to environmental conditions [159]. Application of GA 3 to inflorescences at the pre-bloom stage stimulated flower opening and promoted fruit coloring along with seed abortion in 'Kyoho' grape [158].…”

Section: Gibberellinsmentioning

confidence: 99%

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Recent Advances in Hormonal Regulation and Cross-Talk during Non-Climacteric Fruit Development and Ripening

2019

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Fleshy fruits are characterized by having a developmentally and genetically controlled, highly intricate ripening process, leading to dramatic modifications in fruit size, texture, color, flavor, and aroma. Climacteric fruits such as tomato, pear, banana, and melon show a ripening-associated increase in respiration and ethylene production and these processes are well-documented. In contrast, the hormonal mechanism of fruit development and ripening in non-climacteric fruit, such as strawberry, grape, raspberry, and citrus, is not well characterized. However, recent studies have shown that non-climacteric fruit development and ripening, involves the coordinated action of different hormones, such as abscisic acid (ABA), auxin, gibberellins, ethylene, and others. In this review, we discuss and evaluate the recent research findings concerning the hormonal regulation of non-climacteric fruit development and ripening and their cross-talk by taking grape, strawberry, and raspberry as reference fruit species.

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Section: Gibberellinsmentioning

confidence: 99%

Section: Gibberellinsmentioning

confidence: 99%

Recent Advances in Hormonal Regulation and Cross-Talk during Non-Climacteric Fruit Development and Ripening

2019

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“…In grapevine, which is the first fruit crop to have its entire genome sequenced [25,26], transcriptome sequencing has been conducted to identify microRNAs that are responsive to GA 3 application [27]. Unfortunately, information regarding large-scale transcriptome alterations in response to exogenous GA 3 application in grape remains scarce.…”

Section: Introductionmentioning

confidence: 99%

Gibberellin-induced changes in the transcriptome of grapevine (Vitis labrusca × V. vinifera) cv. Kyoho flowers

et al. 2015

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BackgroundGibberellins are well known for their growth control function in flower, fruit and seed development, and as such, exogenous gibberellic acid (GA) application plays an important role in viticulture. Unfortunately, the mechanism by which GA3 acts in the regulation of these complicated developmental processes in grape remains unclear.ResultsIn the present study, we demonstrated that application of GA3 to ‘Kyoho’ grapevine inflorescences at pre-bloom promoted flower opening, and induced fruit coloring as well as seed abortion. In an attempt to obtain a deeper understanding of the molecular mechanisms driving these responses to GA3 treatment, we performed large-scale transcriptome sequencing of grape flowers following GA3 treatment using Illumina sequencing technology. Global expression profiles of GA3-treated and untreated grape flowers were compared and a large number of GA3-responsive genes were identified. Gene ontology (GO) term classification and biochemical pathway analyses indicated that GA3 treatment caused changes in the levels of transcripts involved in cellular processes, reproduction, hormone and secondary metabolism, as well as the scavenging and detoxification of reactive oxygen species (ROS). These findings suggest that GA3-induced morphological alterations may be related to the control of hormone biosynthesis and signaling, regulation of transcription factors, alteration of secondary metabolites, and the stability of redox homeostasis.ConclusionsTaken together, this comprehensive inflorescence transcriptome data set provides novel insight into the response of grape flowers to GA3 treatment, and also provides possible candidate genes or markers that could be used to guide future efforts in this field.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1324-8) contains supplementary material, which is available to authorized users.

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“…Very recently, Di Genova et al (2014) sequenced a table grape cultivar and compared it to the reference genome of the genotype PN40024 (Jaillon et al, 2007), leading to the identification of 240 novel genes, as well as numerous structural variants and SNPs. In addition, transcriptome analyses were performed by RNA-seq (Venturini et al, 2013;Zenoni et al, 2010) and small RNAs libraries were obtained (Carra et al, 2009;Han et al, 2014;Mica et al, 2010;Pantaleo et al, 2010;Wang et al, 2011). This genetic information could be exploited to identify genes or elucidate pathways involved in traits of agronomic importance (Di Gaspero and Cattonaro, 2010).…”

Section: Introductionmentioning

confidence: 99%

Transient expression assays in grapevine: a step towards genetic improvement

Jelly

Valat

Walter

et al. 2014

Plant Biotechnology Journal

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SummaryIn the past few years, the usefulness of transient expression assays has continuously increased for the characterization of unknown gene function and metabolic pathways. In grapevine (Vitis vinifera L.), one of the most economically important fruit crops in the world, recent systematic sequencing projects produced many gene data sets that require detailed analysis. Due to their rapid nature, transient expression assays are well suited for large-scale genetic studies. Although genes and metabolic pathways of any species can be analysed by transient expression in model plants, a need for homologous systems has emerged to avoid the misinterpretation of results due to a foreign genetic background. Over the last 10 years, various protocols have thus been developed to apply this powerful technology to grapevine. Using cell suspension cultures, somatic embryos, leaves or whole plantlets, transient expression assays enabled the study of the function, regulation and subcellular localization of genes involved in specific metabolic pathways such as the biosynthesis of phenylpropanoids. Disease resistance genes that could be used for marker-assisted selection in conventional breeding or for stable transformation of elite cultivars have also been characterized. Additionally, transient expression assays have proved useful for shaping new tools for grapevine genetic improvement: synthetic promoters, silencing constructs, minimal linear cassettes or viral vectors. This review provides an update on the different tools (DNA constructs, reporter genes, vectors) and methods (Agrobacteriummediated and direct gene transfer methods) available for transient gene expression in grapevine. The most representative results published thus far are then described.

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Grapevine microRNAs responsive to exogenous gibberellin

Cited by 51 publications

References 39 publications

Recent Advances in Hormonal Regulation and Cross-Talk during Non-Climacteric Fruit Development and Ripening

Recent Advances in Hormonal Regulation and Cross-Talk during Non-Climacteric Fruit Development and Ripening

Gibberellin-induced changes in the transcriptome of grapevine (Vitis labrusca × V. vinifera) cv. Kyoho flowers

Transient expression assays in grapevine: a step towards genetic improvement

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