Grapevine Phenological Quantitative Trait SSR Genotyping Using
High-Throughput HRM-PCR Analysis

Awad, Murad K.; Mylona, Photini V.; Polidoros, Alexios N.

doi:10.32604/phyton.2020.011235

Cited by 2 publications

(1 citation statement)

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“…SSRs in amplicon sequences can result in different melting curves. Despite the efficiency of this technique, few applications have been reported for grapevine discrimination due to the complicated necessity of having a good positive control [69][70][71]. Regarding the PCR-based tools dedicated to epigenome profiling, MSAP (methylation-sensitive amplified polymorphism) [72] markers are the most popular although scantily applied to grapevines.…”

Section: Pcr-based Techniquesmentioning

confidence: 99%

DNA-Based Technologies for Grapevine Biodiversity Exploitation: State of the Art and Future Perspectives

et al. 2022

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The cultivated grapevine, Vitis vinifera subsp. vinifera L., is represented by an enormous population of varieties and clones. They arise from the accumulation of gametic and somatic mutations during centuries of sexual and asexual propagation. These varieties represent a vast reservoir of traits/alleles that could be useful in improving the berry quality as well as against environmental stresses. However, most of them are still unexploited. For this reason, an efficient characterization system is essential to define the varietal identity, avoid cases of synonymy (identical genotypes but different names) and homonymy (same names but different genotypes) and deepen our understanding of the existing diversity within the grape germplasm. The plethora of DNA-based high-throughput technologies currently available provides promising tools for the analysis of diversity, overcoming many of the limitations of phenotypic-based diversity analyses. However, the analysis of intra-varietal diversity remains challenging. In this scenario, after summarizing the causes and consequences of grapevine genetic inter- and intra-varietal diversity, we review the DNA-based technologies used for varietal genotyping, emphasizing those able to distinguish clones within a variety. This review provides an update on the technologies used to explore grapevine diversity, the knowledge of which is necessary for an efficient exploitation and conservation of the grapevine germplasm.

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