Grapevine rupestris stem pitting-associated virus

First report on the occurence of grapevine rupestris stem pitting-associated virus in Moroccan grapevines

“…To our knowledge, this is the first report of GRSPaV infecting grapevine in Morocco. Even if the number of the tested samples was relatively low, this result can help to understand the incidence of GRSPaV that seems to be unexpectedly low if compared to other reports (Meng and Rowhani 2017). Due to the selfrooted condition of the old vineyard visited in the Meknes area, this low GRSPaV infection rate (five positive samples out of 35 tested) is conceivable, as this virus essentially spreads through vegetative propagation of infected sources or grafting with infected rootstocks.…”

“…Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus in the family Betaflexiviridae, has a high genetic variability (Meng and Rowhani 2017); some strains are putatively associated with Rupestris stem-pitting disease, a component of the Rugose Wood complex of the grapevine. To assess the viral status of grapevine in Meknès region (the central part of Morocco), a total of 35 samples of cv.…”

Genetic diversity and identification of putative recombination events in grapevine rupestris stem pitting-associated virus

“…Representation of the complete GRSPaV genome. MTR, methyltransferase; HVR, highly variable region, AlkB, alkylation B; OTU, ovarian tumour-like cysteine protease; P-Pro, papainlike cysteine protease; HEL, helicase; POL, RNA-dependent RNA polymerase; TGB, triple gene block; CP, capsid protein (Meng and Rowhani, 2017) Sequences derived from whole genomes are indicated with hash signs (#). Sequences for which a partial genome was available, are indicated with an asterisk (*).…”

“…Phylogenetic analyses were performed using the Neighbour Joining algorithm. Nodes with bootstrap values of less than 50% were collapsed (Meng and Rowhani, 2017). Corresponding lineages according to Hu et al (2015) Nodes with bootstrap values of less than 50% were collapsed (Meng and Rowhani, 2017).…”

“…The ORFs are flanked by two non-coding regions (NCRs): a 60 nt 5' NCR which is presumably capped, and a 175 nt polyadenylated 3' NCR ( Figure 6). Each NCR contains invariable sites essential for virus replication (Meng and Rowhani, 2017). The genome structure is identical for all isolates for which the full genome sequences are available (Giampetruzzi et al, 2012;Hu et al, 2015;Lima et al, 2009Lima et al, , 2006aLima et al, , 2006bMeng et al, 2005Meng et al, , 1998Morelli et al, 2011;Poojari et al, 2013;Zhang et al, 1998).…”