Graphene based aptasensor for glycated albumin in diabetes mellitus diagnosis and monitoring

Apiwat, Chayachon; Luksirikul, Patraporn; Kankla, Pacharapon; Pongprayoon, Prapasiri; Treerattrakoon, Kiatnida; Paiboonsukwong, Kittiphong; Fucharoen, Suthat; Dharakul, Tararaj; Japrung, Deanpen

doi:10.1016/j.bios.2016.04.015

Cited by 84 publications

(67 citation statements)

References 25 publications

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“…The K d value of GABAS-01 was 1.748 ± 0.227 nM, and the LOD was 16.40 μg/mL. Although the aptamers specific for GHSA have been reported, GABAS-01 has higher sensitivity than previously reported aptamers [ 38 ]. This result suggests the potential applications of GABAS-01 as an effective on-site detection probe of GHSA.…”

Section: Discussionmentioning

confidence: 96%

“…However, this method usually has relatively low sensitivities ( Table 4 ). Aptasensor systems based on fluorescence have also been developed [ 38 ]; this graphene methodology is almost the same as our system and the LOD of this aptamer system is 50 μg/mL. Our system’s LOD is 16.40 μg/mL and it is more sensitive.…”

Section: Discussionmentioning

confidence: 97%

“…The K d value of GABAS-01 indicates that GABAS-01 has a high binding affinity for GHSA. This value is much lower than those of the other aptamers for glycated human serum albumin, which displayed affinities in the micromolar range [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…Generally, graphene oxide (GO) can adsorb single-stranded oligonucleotides through π-π stacking interactions and it requires simple detachment methods to separate the adsorbed single-stranded oligonucleotides from graphene oxide [ 36 , 37 ]. The detachment methods need addition of complementary sequences or other molecules such as proteins that lead to conformational changes, because the interactions between the single-stranded oligonucleotides and GO are the result of the exposed phosphate backbone [ 36 , 37 , 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

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Development of a Novel ssDNA Sequence for a Glycated Human Serum Albumin and Construction of a Simple Aptasensor System Based on Reduced Graphene Oxide (rGO)

et al. 2020

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Diabetes is one of the top 10 global causes of death. About one in 11 global adults have diabetes. As the disease progresses, the mortality rate increases, and complications can develop. Thus, early detection and effective management of diabetes are especially important. Herein, we present a novel glycated human serum albumin (GHSA) aptamer, i.e., GABAS-01, which has high affinity and specificity. The aptamer was selected by reduced graphene oxide-based systematic evolution of ligands by exponential enrichement (rGO-based SELEX) against GHSA. After five rounds of selection through gradually harsher conditions, GABAS-01 with high affinity and specificity for the target was obtained. GABAS-01 was labeled by FAM at the 5′-end and characterized by measuring the recovery of a fluorescence signal that is the result of fluorescence quenching effect of rGO. As a result, GABAS-01 had low-nanomolar Kd values of 1.748 ± 0.227 nM and showed a low limit of detection of 16.40 μg/mL against GHSA. This result shows the potential application of GABAS-01 as an effective on-site detection probe of GHSA. In addition, these properties of GABAS-01 are expected to contribute to detection of GHSA in diagnostic fields.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of a Novel ssDNA Sequence for a Glycated Human Serum Albumin and Construction of a Simple Aptasensor System Based on Reduced Graphene Oxide (rGO)

et al. 2020

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“…5). Besides, there are widely used electrophoretic mobility shift assay (EMSA) (6), Differential Radial Capillary Action of Ligand Assay (DRa-CALA) (7) and ELISA-like assay based on aptamer (ELASA) with fluorescent (8) or colorimetric (9) detection. Some of them are time-or cost-consuming, the other ones require complicated equipment and highly qualified staff.…”

Section: Introductionmentioning

confidence: 99%

The Ca²⁺‐Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation

Krasitskaya

Гончарова

Бирюков

et al. 2020

Photochem & Photobiology

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Bioluminescent solid‐phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer–target complexes during design and development of the specific aptamers. The assay involves Ca2+‐regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.

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Graphene‐Based Electrochemical Aptasensors

Özalp

Karabiyik

Bayraç

et al. 2019

Handbook of Graphene

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Graphene based aptasensor for glycated albumin in diabetes mellitus diagnosis and monitoring

Cited by 84 publications

References 25 publications

Development of a Novel ssDNA Sequence for a Glycated Human Serum Albumin and Construction of a Simple Aptasensor System Based on Reduced Graphene Oxide (rGO)

Development of a Novel ssDNA Sequence for a Glycated Human Serum Albumin and Construction of a Simple Aptasensor System Based on Reduced Graphene Oxide (rGO)

The Ca²⁺‐Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation

Graphene‐Based Electrochemical Aptasensors

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Graphene based aptasensor for glycated albumin in diabetes mellitus diagnosis and monitoring

Cited by 84 publications

References 25 publications

Development of a Novel ssDNA Sequence for a Glycated Human Serum Albumin and Construction of a Simple Aptasensor System Based on Reduced Graphene Oxide (rGO)

Development of a Novel ssDNA Sequence for a Glycated Human Serum Albumin and Construction of a Simple Aptasensor System Based on Reduced Graphene Oxide (rGO)

The Ca2+‐Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation

Graphene‐Based Electrochemical Aptasensors

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Product

Resources

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The Ca²⁺‐Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation