Graphene‐Based Patterning and Differentiation of C2C12 Myoblasts

Bajaj, Preeti; Rivera, José A.; Marchwiany, Daniel; Solovyeva, Vita; Bashir, Rashid

doi:10.1002/adhm.201300550

Cited by 46 publications

(56 citation statements)

References 38 publications

(49 reference statements)

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“…8) [178, 179]. When C2C12 myoblasts were grown on ultrathin thermally reduced (TR) graphene-based films, cells demonstrated higher attachment and better spreading behavior than GO and glass slide [178].…”

Section: Applications In Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

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Graphene-based materials for tissue engineering

Shin

Jang

et al. 2016

Advanced Drug Delivery Reviews

580

418

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Graphene and its chemical derivatives have been a pivotal new class of nanomaterials and a model system for quantum behavior. The material's excellent electrical conductivity, biocompatibility, surface area and thermal properties are of much interest to the scientific community. Two dimensional graphene materials have been widely used in various biomedical research areas such as bioelectronics, imaging, drug delivery, and tissue engineering. In this review we will highlight the recent applications of graphene-based materials in tissue engineering and regenerative medicine. In particular, we will discuss the application of graphene-based materials in cardiac, neural, bone, cartilage, skeletal muscle, and skin/adipose tissue engineering. We also discuss the potential risk factors of graphene-based materials in tissue engineering. In addition, we will outline the opportunities in the usage of graphene-based materials for clinical applications.

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Section: Applications In Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

“…Graphene-based materials can also enhance cellular alignment and thus maximize cellular contractile power for skeletal muscle tissues [179, 180]. Based on the attachment preference of C2C12 cells on graphene over SiO 2 .…”

Section: Applications In Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

Graphene-based materials for tissue engineering

Shin

Jang

et al. 2016

Advanced Drug Delivery Reviews

580

418

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“…As myotubes, C2C12 cells are electrically active and have been shown to respond to applied electrical stimulus. 5,24,34-36 Previous reports have used fluorescent Ca 2+ imaging to identify C2C12 functionality as well as studies with neural cells on GF. 21 By identifying changes in fluorescence intensity and position triggered by a series of voltage pulses, we reveal the functionality of differentiated myotube cells on GF.…”

Section: Resultsmentioning

confidence: 99%

“…Analysis of the myotube size shown in Reference 5 reveal an average length of 602 ± 117 μm and width of 26 ± 3 μm. The myotubes on the laminin coated GF had an average length of 144 ± 38 μm and width of 6 ± 3 μm (Figures 6A and 6B).…”

Section: Discussionmentioning

confidence: 99%

Graphene Foam as a Three-Dimensional Platform for Myotube Growth

Krueger

Chang

Brown

et al. 2016

ACS Biomater. Sci. Eng.

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This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on 3-dimensional graphene foam bioscaffolds. Specifically, we establish both bare and laminin coated graphene foam as a biocompatible platform for muscle cells and identify that electrical coupling stimulates cell activity. Cell differentiation and functionality is determined by the expression of myotube heavy chain protein and Ca2+ fluorescence, respectively. Further, our data show that the application of a pulsed electrical stimulus to the graphene foam initiates myotube contraction and subsequent localized substrate movement of over 100 micrometers. These findings will further the development of advanced 3-dimensional graphene platforms for therapeutic applications and tissue engineering.

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“…In field of tissue engineering, there are many methods to fabricate the muscle tissue engineering scaffolds, such as molding, photoresist self-assembly method, electrostatic spinning, etc. Through cultivating the scaffolds which are seeded cells to stimulate the cells differentiation for implementing structure and part of the muscle function [7][8][9]. The function of the microfludic network is very important which provides the exchange of nutrients and space for cell production and migration.…”

Section: Introductionmentioning

confidence: 99%