Graphene oxide and gold nanoparticle based dual platform with short DNA probe for the PCR free DNA biosensing using surface-enhanced Raman scattering

Khalil, Ibrahim; Yehye, Wageeh A.; Julkapli, Nurhidayatullaili Muhd; Rahmati, Shahrooz; Sina, Abu Ali Ibn; Basirun, Wan Jefrey; Johan, Mohd Rafie

doi:10.1016/j.bios.2019.02.028

Cited by 77 publications

(46 citation statements)

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“…A slightly different experimental approach was shown by Braun et al, where capture DNA was immobilized on a smooth metal film together with the RR, so that the SERS signal could not yet be recorded, and then, during the hybridization process, the target DNA, together with the reporter DNA immobilized on the silver nanoparticles, formed a structure with a much larger cross section for Raman scattering, as the RR was located between the silver nanoparticles and the flat silver film forming a silver sandwich structure with the dye inside [97]. A two-platform sensor based on gold nanoparticles was presented by Khalil and co-workers [98]. Graphene oxide-gold nanoparticles with immobilized thiolated capture DNA and complementary target DNA were deposited as the first element, whereas the second element was built from capture DNA and Cy3 adsorbed on the gold nanoparticles.…”

Section: Sandwich Sensors With Rr Located On Nanoparticlesmentioning

confidence: 99%

“…A two-platform sensor based on gold nanoparticles was presented by Khalil and co-workers [98]. Graphene oxide-gold nanoparticles with immobilized thiolated capture DNA and complementary target DNA were deposited as the first element, whereas the second element was built from capture DNA and Cy3 adsorbed on the gold nanoparticles.…”

Section: Sandwich Sensors With Rr Located On Nanoparticlesmentioning

confidence: 99%

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Surface Enhanced Raman Spectroscopy for DNA Biosensors—How Far Are We?

et al. 2019

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A sensitive and accurate identification of specific DNA fragments (usually containing a mutation) can influence clinical decisions. Standard methods routinely used for this type of detection are PCR (Polymerase Chain Reaction, and its modifications), and, less commonly, NGS (Next Generation Sequencing). However, these methods are quite complicated, requiring time-consuming, multi-stage sample preparation, and specially trained staff. Usually, it takes weeks for patients to obtain their results. Therefore, different DNA sensors are being intensively developed by many groups. One technique often used to obtain an analytical signal from DNA sensors is Raman spectroscopy. Its modification, surface-enhanced Raman spectroscopy (SERS), is especially useful for practical analytical applications due to its extra low limit of detection. SERS takes advantage of the strong increase in the efficiency of Raman signal generation caused by a local electric field enhancement near plasmonic (typically gold and silver) nanostructures. In this condensed review, we describe the most important types of SERS-based nanosensors for genetic studies and comment on their potential for becoming diagnostic tools.

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Section: Sandwich Sensors With Rr Located On Nanoparticlesmentioning

confidence: 99%

Section: Sandwich Sensors With Rr Located On Nanoparticlesmentioning

confidence: 99%

Surface Enhanced Raman Spectroscopy for DNA Biosensors—How Far Are We?

et al. 2019

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“…The introducing of ATP can form a sandwich structure that brought the GO/Au nanolayer and the AuNPs in close proximity, and the enhanced SERS signal allowed the sensitive quantification of ATP with a detection limit of 0.85 pM. GO and AuNPs-based SERS platforms for DNA biosensing was reported ( Figure 14) [45]. The capture probe 1 was functionalized by GO-AuNPs and capture probe 2 was modified by Raman dye and AuNPs.…”

Section: Graphene-based Sers Biosensorsmentioning

confidence: 99%

mentioning

confidence: 99%

Graphene-Based Biosensors for Detection of Biomarkers

2020

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The development of biosensors with high sensitivity and low-detection limits provides a new direction for medical and personal care. Graphene and graphene derivatives have been used to prepare various types of biosensors due to their excellent sensing performance (e.g., high specific surface area, extraordinary electronic properties, electron transport capabilities and ultrahigh flexibility). This perspective review focuses on graphene-based biosensors for quantitative detection of cancer-related biomarkers such as DNA, miRNA, small molecules and proteins by integrating with different signal outputting approaches including fluorescent, electrochemistry, surface plasmon resonance, surface enhanced Raman scattering, etc. The article also discussed their challenges and potential solutions along with future prospects.

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Section: Applications Of Different Types Of Graphene-based Biosensorsmentioning

confidence: 99%

Graphene-Based Biosensors for Detection of Biomarkers

Bai¹,

Xu²,

Zhang³

2019

Preprint

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The development of biosensors with high sensitivity and low-detection limits provides a new direction for medical and personal care. Graphene and graphene derivatives have been used to prepare various types of biosensors due to their excellent sensing performance (e.g. high specific surface area, extraordinary electronic properties, electron transport capabilities and ultrahigh flexibility). This perspective review focuses on graphene-based biosensors for quantitative detection of cancer related biomarkers such as DNA, miRNA, small molecules and proteins by integrating with different signal outputting approaches including fluorescent, electrochemistry, surface plasmon resonance, surface enhanced Raman scattering etc. The article also discussed their challenges and potential solutions along with future prospects.

show abstract

Graphene oxide and gold nanoparticle based dual platform with short DNA probe for the PCR free DNA biosensing using surface-enhanced Raman scattering

Cited by 77 publications

References 50 publications

Surface Enhanced Raman Spectroscopy for DNA Biosensors—How Far Are We?

Surface Enhanced Raman Spectroscopy for DNA Biosensors—How Far Are We?

Graphene-Based Biosensors for Detection of Biomarkers

Graphene-Based Biosensors for Detection of Biomarkers

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