Graphene Oxide Based Fluorescent DNA Aptasensor for Liver Cancer Diagnosis and Therapy

Abstract

Graphene-oxide based fluorescent DNA aptasensors for the in vivo application remain a challenging task. We demonstrate for the first time such a nanomaterial for in vivo diagnosis and therapy of liver tumor with good biocompatibility and high selectivity. This DNA nanomaterial comprising of DNA tetrahedron and aptamers, aggregation-induced emission luminogen (AIEgens) and antitumor drug doxorubicin, is fabricated and attached on GO surface. Additionally, this GO-based fluorescent DNA nanodevice is also constructed by using microfluidic chip for liver tumor cell screening. File list (2) download file view on ChemRxiv Manuscript 20210308-Ke净版.docx (2.26 MiB) download file view on ChemRxiv Manuscript 20210308-Ke净版.pdf (1.27 MiB)

Photoluminescence and Fluorescence Quenching of Graphene Oxide: A Review

“…Tang et al have developed a GO-based DNA nanomaterial for cancer diagnosis and treatment in vitro and in vivo [ 102 ]. This material can be used for organ imaging in vivo, especially for liver tumors ( Figure 17 a).…”

“… ( a ) Organ imaging 24 h after injection of GO–DNA nanomaterials. Reprinted with permission from [ 102 ]. Copyright 2021, John Wiley and Sons.…”

“…Howev biodegradability and has a certain toxicity, even though it has many excel [99]. In contrast, GO does not only have the advantage of negligible tox provides feedback on drug loading and release due to its optical propertie Tang et al have developed a GO-based DNA nanomaterial for cancer treatment in vitro and in vivo [102]. This material can be used for organ im Sastikumar et al successfully synthesized non-toxic fluorescent NGOs by laser ablation of graphene solution and applied them to the field of biological imaging [98].…”

Microfluidic nanodevices for drug sensing and screening applications

“…In another study, DNA tetrahedron and aptamer-based aggregation induced the emission of luminescence, and these aggregated molecules were further modified with the anticancer drug DOX. Moreover, these modified molecules were attached to the surface of the graphene oxide layer, and the resulting sensing platform possessed higher selectivity for liver cancer cells ( Ma et al, 2021 ). As shown in Fig.…”

“…(a) Screening of bisphenol A based on DNA functionalized graphene with microfluidic device, reproduced with permission from ( Liu et al, 2018 ), copyright 2018 American Chemical Society. (b) DNA-modified graphene oxide-based microfluidic for the diagnosis and treatment of liver cancer cells, reproduced with permission from ( Ma et al, 2021 ), copyright 2021 Wiley-VCH. (c) Detection of serum cocaine based on aptamer-modified microfluidic system, reproduced with permission from ( Oueslati et al, 2018 ), copyright 2018 Elsevier.…”