Graphene Oxide Improves in vitro Fertilization in Mice With No Impact on Embryo Development and Preserves the Membrane Microdomains Architecture

Bernabò, Nicola; Valbonetti, Luca; Raspa, Marcello; Fontana, Antonella; Palestini, Paola; Botto, Laura; Paoletti, Renata; Fray, Martin; Allen, Susan; Machado-Simoes, Juliana; Ramal-Sanchez, Marina; Pilato, Serena; Scavizzi, Ferdinando; Barboni, Barbara

doi:10.3389/fbioe.2020.00629

Cited by 11 publications

(6 citation statements)

References 64 publications

(67 reference statements)

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“…An a priori power analysis was done to establish the number of oocytes with G*Power 3.1.9.7 software to get a final power of our analysis ≥95%. The results were expressed as the difference between the CTRL and treated samples, expressed as a percentage on the CTRL (∆ IVF% ) [40]. To analyze the IVF data, Tukey's and Dunnett's multiple comparison tests were used.…”

Section: Discussionmentioning

confidence: 99%

Pre-Treatment of Swine Oviductal Epithelial Cells with Progesterone Increases the Sperm Fertilizing Ability in an IVF Model

Cimini

Moussa

Taraschi

et al. 2022

Animals

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Mammalian spermatozoa are infertile immediately after ejaculation and need to undergo a functional modification, called capacitation, in order to acquire their fertilizing ability. Since oviductal epithelial cells (SOECs) and progesterone (P4) are two major modulators of capacitation, here we investigated their impact on sperm functionality by using an IVF swine model. To that, we treated SOECs with P4 at 10, 100, and 1000 ng/mL before the coincubation with spermatozoa, thus finding that P4 at 100 ng/mL does not interfere with the cytoskeleton dynamics nor the cells’ doubling time, but it promotes the sperm capacitation by increasing the number of spermatozoa per polyspermic oocyte (p < 0.05). Moreover, we found that SOECs pre-treatment with P4 100 ng/mL is able to promote an increase in the sperm fertilizing ability, without needing the hormone addition at the time of fertilization. Our results are probably due to the downregulation in the expression of OVGP1, SPP1 and DMBT1 genes, confirming an increase in the dynamism of our system compared to the classic IVF protocols. The results obtained are intended to contribute to the development of more physiological and efficient IVF systems.

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Section: Discussionmentioning

confidence: 99%

Pre-Treatment of Swine Oviductal Epithelial Cells with Progesterone Increases the Sperm Fertilizing Ability in an IVF Model

Cimini

Moussa

Taraschi

et al. 2022

Animals

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“…There are two types of CNTs, namely single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs), as identified by Al Moustafa et al in 2016. Thanks to their exceptional mechanical, electrical, and thermal features, they serve different purposes, one of which is embryo manipulation. Meanwhile, another carbon-based nanomaterial that holds potential is graphene oxide or GO, obtained from graphene by chemically oxidizing graphite flakes with strong oxidizing agents as noted by Liao et al in 2018. Studies suggest that GO can improve in vitro fertilization in mice without impacting embryo development (Bernabo et al, 2020). As such, carbon-based nanomaterials have displayed promising outcomes in enhancing embryo manipulation techniques (Gaur et al, 2021).…”

Section: Types Of Nanomaterials Used In Embryo Manipulationsmentioning

confidence: 99%

The Positive Effects of In Vivo/In Vitro Suplementation of Nanoparticles on Semen

Koca

2023

Nanotechnology in Reproduction

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Nanoparticles (NPs) are small size molecules. It is divided into organic and inorganic. Organic NPs have many clinical uses, from inoculation to homeostasis, long-term storage and delivery to the system, and absorption of topically used drugs into the skin. Inorganic NPs can be used in intraoperative lymphatic scanning, tumors, and anemia treatments. NPs have a favorable effect on sperm motility, kinematic parameters or membrane integrity rates, they can also be used as antibacterial, antiviral and antifungal in genital organ diseases. There are many studies in which the addition of NPs can be beneficial or harmful to sperm. Researchers have stated that it may be beneficial or cytotoxic depending on in vivo (such as airway, skin or injection) or in vitro use. It has been reported that NPs have positive effects on motility, kinematic parameters, plasma membrane integrity, DNA damage and acrosomal integrity and can be used as an antioxidant. In addition, it has been reported that it can be used in sexsorting, bioimaging and nanopurification, and that more studies on NPs will increase in the coming years.

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“…As a result, 29 documents (26 articles and 3 reviews, no publications emerge before 2012) were found published from 2012 to 2019 (see Table 1 ). Since data were downloaded until March 31th, 2020, and due to the limitations of the Software in terms of sensibility, two documents were lost and were not included within the analysis: Kong et al, 2019 [ 28 ] and Bernabò et al, 2020 [ 29 ]. Thus, the total number of documents will reach 31 (28 articles and 3 reviews) even if only those identified by the system were included.…”

Section: Scientometric Analysis Of Graphene and Gdms And The Repromentioning

confidence: 99%

“…The results showed that the addition of 0.5 μg/mL GO to a sperm suspension before performing IVF was able to increase both the number of fertilized oocytes and, surprisingly, the birth rate after embryo transplantation in foster mothers, in a more effective way than the gold standard in promoting in vitro fertility of mice spermatozoa (methyl-β-cyclodextrin). Furthermore, it was demonstrated that GO exerts its positive effect by extracting cholesterol from the membranes, without affecting the integrity of the membrane microdomains and thus preserving the sperm function [ 29 ].…”

Section: Graphene and Reproduction: Toxicological Studiesmentioning

confidence: 99%

Graphene and Reproduction: A Love-Hate Relationship

et al. 2021

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Since its discovery, graphene and its multiple derivatives have been extensively used in many fields and with different applications, even in biomedicine. Numerous efforts have been made to elucidate the potential toxicity derived from their use, giving rise to an adequate number of publications with varied results. On this basis, the study of the reproductive function constitutes a good tool to evaluate not only the toxic effects derived from the use of these materials directly on the individual, but also the potential toxicity passed on to the offspring. By providing a detailed scientometric analysis, the present review provides an updated overview gathering all the research studies focused on the use of graphene and graphene-based materials in the reproductive field, highlighting the consequences and effects reported to date from experiments performed in vivo and in vitro and in different animal species (from Archea to mammals). Special attention is given to the oxidized form of graphene, graphene oxide, which has been recently investigated for its ability to increase the in vitro fertilization outcomes. Thus, the potential use of graphene oxide against infertility is hypothesized here, probably by engineering the spermatozoa and thus manipulating them in a safer and more efficient way.

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Graphene Oxide Improves in vitro Fertilization in Mice With No Impact on Embryo Development and Preserves the Membrane Microdomains Architecture

Cited by 11 publications

References 64 publications

Pre-Treatment of Swine Oviductal Epithelial Cells with Progesterone Increases the Sperm Fertilizing Ability in an IVF Model

Pre-Treatment of Swine Oviductal Epithelial Cells with Progesterone Increases the Sperm Fertilizing Ability in an IVF Model

The Positive Effects of In Vivo/In Vitro Suplementation of Nanoparticles on Semen

Graphene and Reproduction: A Love-Hate Relationship

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