Graphene Oxide Modified Chemically Activated Graphite Electrodes for Detection of microRNA

Erdem, Arzum; Ekşin, Ece; Isin, D. K.; Polat, Derya Çiçek

doi:10.1002/elan.201600761

Cited by 29 publications

(23 citation statements)

References 49 publications

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“…After IL modification onto CA/PGE surface, 93.83% decrease at R ct was recorded ( Figure 2 b,c). This decrease is consistent with the fact that the IL modification can yield an increase at the conductivity of PGE surface since there is an increase at the interfacial electron transfer occurring between the electrode and the electroactive species in solution [ 31 ].…”

Section: Resultssupporting

confidence: 77%

“…PGEs were electrochemically pretreated by applying +1.40 V for 30 s in acetate buffer solution (ABS, pH 4.80) ( Figure 1 i) [ 6 , 7 , 13 , 31 , 32 ]. Each pretreated PGE was immersed into the vials containing 100 μL of 5 mM CA solution during 60 min to obtain activated carboxyl groups at the surface of the PGEs [ 31 ] before IL modification. Each PGEs was then rinsed with PBS for 10 s to remove unbound probe from electrode surface ( Figure 1 ii).…”

Section: Methodsmentioning

confidence: 99%

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An Impedimetric Biosensor Based on Ionic Liquid-Modified Graphite Electrodes Developed for microRNA-34a Detection

Kesici

Ekşin

Erdem

2018

Sensors

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In the present work, an impedimetric nucleic acid biosensor has been designed for the purpose of detection of microRNA (miRNA). Ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate (IL))-modified chemically activated pencil graphite electrodes (PGEs) were used for the sensitive and selective detection of miRNA-34a. After covalent activation of the PGE surface using covalent agents (CAs), the ionic liquid (IL) was immobilized onto the surface of the chemically activated PGE by passive adsorption. The electrochemical and microscopic characterization of the IL/CA/PGEs was performed by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and scanning electron microscopy (SEM). DNA probe concentration, miRNA target concentration, and also the hybridization time and wet adsorption time were optimized by using the EIS technique. Then, the hybridization occurred between specific DNA probes and miRNA-34a was immobilized onto the surface of the IL/CA/PGEs. The impedimetric detection of miRNA-DNA hybrid was performed by EIS. The detection limit (DL) was calculated in a linear concentration range of 2–10 µg/mL miRNA-34a target, and it was found to be 0.772 µg/mL (109 nM) in phosphate buffer solution (PBS) and 0.826 µg/mL (117 nM) in diluted fetal bovine serum (FBS). The selectivity of impedimetric biosensor for miRNA-34a was also tested against to other non-complementary miRNA sequences both in buffer media, or diluted FBS.

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Section: Resultssupporting

confidence: 77%

Section: Methodsmentioning

confidence: 99%

An Impedimetric Biosensor Based on Ionic Liquid-Modified Graphite Electrodes Developed for microRNA-34a Detection

Kesici

Ekşin

Erdem

2018

Sensors

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“…Also, the use graphene microelectrodes for the detection of a wide range of analytes such as microRNA etc. have appeared in the literature recently [27].…”

Section: Examples Of Biosensors Based On Lipid Modified Graphene Micrmentioning

confidence: 99%

Biosensors Based on Lipid Modified Graphene Microelectrodes

Nikoleli

Siontorou

Nikolelis

et al. 2017

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Graphene is one of the new materials which has shown a large impact on the electronic industry due to its versatile properties, such as high specific surface area, high electrical conductivity, chemical stability, and large spectrum of electrochemical properties. The graphene material-based electronic industry has provided flexible devices which are inexpensive, simple and low power-consuming sensor tools, therefore opening an outstanding new door in the field of portable electronic devices. All these attractive advantages of graphene give a platform for the development of a new generation of devices in both food and environmental applications. Lipid-based sensors have proven to be a good route to the construction of novel devices with improved characteristics, such as fast response times, increased sensitivity and selectivity, and the possibility of miniaturization for the construction of portable biosensors. Therefore, the incorporation of a lipid substrate on graphene electrodes has provided a route to the construction of a highly sensitive and selective class of biosensors with fast response times and portability of field applications for the rapid detection of toxicants in the environment and food products.

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“…There have been many studies based on electrochemical detection for miRNA detection in the literature . Li et al.…”

Section: Introductionmentioning

confidence: 99%

“…There have been many studies based on electrochemical detection for miRNA detection in the literature [35][36][37][38][39][40][41][42][43][44][45][46]. Li et al [31] developed label-free electrochemical biosensor for miRNAs detection by measuring the guanine oxidation signal on a multi-walled carbon nanotube (MWCNT) modified glassy carbon electrode (GCE).…”

mentioning

confidence: 99%

Ionic Liquid Modified Single‐use Electrode Developed for Voltammetric Detection of miRNA‐34a and its Application to Real Samples

et al. 2019

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The ionic liquid (IL) modified chemically activated (CA) pencil graphite electrodes (PGEs) were developed for label‐free voltammetric detection of miRNA‐34a, and implemented to the real samples. Firstly, the electrochemical characterization of unmodified PGE, CA‐PGE, IL‐PGE and IL‐CA‐PGE was performed by cyclic voltammetry (CV) as well as their DNA binding capacity was studied by electrochemical impedance spectroscopy (EIS) technique. The microscopic characterization of the surface of each electrodes was investigated by scanning electron microscopy (SEM). Differential pulse voltammetry (DPV) technique was used for measuring the oxidation signal of guanine in order to perform a label‐free voltammetric monitoring of a full‐match hybridization specific to miRNA‐34a. The selectivity of biosensor was tested against to miRNA‐155, miRNA‐660 as well as to the mismatch sequence of miRNA‐34a. The further selectivity of this proposed biosensor was studied in the mixture of samples containing miRNA‐34a with other miRNAs (1 : 1). The voltammetric detection of miRNA‐34a was also explored in the artificial serum medium as fetal bovine serum (FBS) and also in total RNA samples isolated from HUH‐7 human hepatocellular carcinoma cell line.

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Graphene Oxide Modified Chemically Activated Graphite Electrodes for Detection of microRNA

Cited by 29 publications

References 49 publications

An Impedimetric Biosensor Based on Ionic Liquid-Modified Graphite Electrodes Developed for microRNA-34a Detection

An Impedimetric Biosensor Based on Ionic Liquid-Modified Graphite Electrodes Developed for microRNA-34a Detection

Biosensors Based on Lipid Modified Graphene Microelectrodes

Ionic Liquid Modified Single‐use Electrode Developed for Voltammetric Detection of miRNA‐34a and its Application to Real Samples

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