Gravity and lymphodynamics

Holm-Weber, Thomas; Kristensen, Rasmus; Mohanakumar, Sheyanth; Hjortdal, Vibeke E.

doi:10.14814/phy2.15289

Cited by 8 publications

(4 citation statements)

References 29 publications

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“…Screening of reference lists of included papers yielded an additional seven articles for inclusion. In total, 28 papers (Amann-Vesti et al, 2003;Belgrado et al, 2016;Bell et al, 2020;Gray et al, 2016;Groenlund et al, 2017;Holm-Weber et al, 2022;Kelly, Mohanakumar, Telinius, et al, 2020;Lopera et al, 2017;Majgaard et al, 2022;Modi et al, 2007;Mohanakumar et al, 2018Mohanakumar et al, , 2019Mohanakumar et al, , 2020Mohanakumar et al, , 2021Rane et al, 2013;Rasmussen et al, 2010Rasmussen et al, , 2021Rasmussen et al, , 2022Saito et al, 2015;Tan et al, 2011;Telinius et al, 2010Telinius et al, , 2015Telinius et al, , 2017Telinius, Baandrup, et al, 2014;Telinius, Mohanakumar, et al, 2014;Unno et al, 2010;Yamamoto et al, 2014) met the inclusion criteria, see Figure 1. Of these, 20 were in vivo studies, seven were ex vivo and one study contained both in vivo and ex vivo data.…”

Section: Resultsmentioning

confidence: 99%

The transport function of the human lymphatic system—A systematic review

Thorup,

Hjortdal,

Boedtkjer

et al. 2023

Physiological Reports

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Physiological properties and function of the lymphatic system is still somewhat of a mystery. We report the current knowledge about human lymphatic vessel contractility and capability of adaptation. A literature search in PubMed identified studies published January 2000–September 2022. Inclusion criteria were studies investigating parameters related to contraction frequency, fluid velocity, and lymphatic pressure in vivo and ex vivo in human lymphatic vessels. The search returned 2885 papers of which 28 met the inclusion criteria. In vivo vessels revealed baseline contraction frequencies between 0.2 ± 0.2 and 1.8 ± 0.1 min1, velocities between 0.008 ± 0.002 and 2.3 ± 0.3 cm/s, and pressures between 4.5 (range 0.5–9.2) and 60.3 ± 2.8 mm Hg. Gravitational forces, hyperthermia, and treatment with nifedipine caused increases in contraction frequency. Ex vivo lymphatic vessels displayed contraction frequencies between 1.2 ± 0.1 and 5.5 ± 1.2 min−1. Exposure to agents affecting cation and anion channels, adrenoceptors, HCN channels, and changes in diameter‐tension properties all resulted in changes in functional parameters as known from the blood vascular system. We find that the lymphatic system is dynamic and adaptable. Different investigative methods yields alternating results. Systematic approaches, consensus on investigative methods, and larger studies are needed to fully understand lymphatic transport and apply this in a clinical context.

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Section: Resultsmentioning

confidence: 99%

The transport function of the human lymphatic system—A systematic review

Thorup,

Hjortdal,

Boedtkjer

et al. 2023

Physiological Reports

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“…In LabVIEW, a region of interest can detect the changes in light intensity emitted from the ICG dye. Regions of interest were plotted on all visible vessels [ 11 , 13 , 16 ]. The changes in light intensity were measured in the regions of interest to detect lymph propulsions.…”

Section: Methodsmentioning

confidence: 99%

Octreotide improves human lymphatic fluid transport a translational trial

Holm-Weber,

Skov,

Mohanakumar

et al. 2023

European Journal of Cardio-Thoracic Surgery

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Objective Chylothorax is a complex condition and many different pharmacological agents have been tried as treatment. Octreotide is used off-label to treat chylothorax, but the efficacy of octreotide remains unclear. A decrease in lymph production is suggested as the mechanism. In this cross-over study, we explore the direct effect of octreotide on human lymphatic drainage. Methods Pre-clinical: The effect of octreotide on force generation was assessed during acute and prolonged drug incubation on human lymphatic vessels mounted in a myograph. Clinical: In a double-blinded, randomized, cross-over trial including 16 healthy adults, we administered either octreotide or saline as an intravenous infusion for 2.5 hours. Near-Infrared Fluorescence Imaging was used to examine spontaneous lymphatic contractions and lymph pressure in peripheral lymphatic vessels and plethysmography was performed to assess the capillary filtration rate, capillary filtration coefficient, and isovolumetric pressures of the lower leg. Results Pre-clinical: Human thoracic duct (n = 12) contraction rate was concentration-dependently stimulated by octreotide with a maximum effect at 10 and 100 nmol/l in the myograph chamber Clinical: Spontaneous lymphatic contractions and lymph pressure evaluated by NIRF did not differ between octreotide or placebo (P = 0.36). Plethysmography revealed similar capillary filtration coefficients (P = 0.057), but almost a doubling of the isovolumetric pressures (P = 0.005) during octreotide infusion. Conclusion Octreotide stimulated lymphatic contractility in the pre-clinical setup but did not affect the spontaneous lymphatic contractions or lymph pressure in healthy individuals. Plethysmography revealed a doubling in the isovolumetric pressure. These results suggest that octreotide increases lymphatic drainage capacity in situations with high lymphatic afterload.

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“…Blood and lymph velocity varies greatly between region and species, with slower speeds in rodent superficial lymphatics (1-20 µm/s) 51 and capillaries (10-100 µm/s), 52 and greater speeds in human and rodent mesenteric lymphatics (0.1-2 cm/s) 53,54 and human veins (1.5-7.1 cm/s). 55 To test the ability of the multi-tissue chip and pump to connect tissue compartments at physiological speeds, we quantified the velocity through the channel as a function of user-controllable parameters.…”

Section: On-chip Fluid Recirculation At Controllable Speedsmentioning

confidence: 99%

A 3D-printed multi-compartment organ-on-chip platform with a tubing-free pump models communication with the lymph node

Cook,

Ball,

Mohammad

et al. 2024

Preprint

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Multi-organ-on-chip systems (MOOCs) have the potential to mimic communication between organ systems and reveal mechanisms of health and disease. However, many existing MOOCs are challenging for non-experts to implement, due to complex tubing, electronics, or pump mechanisms. In addition, few MOOCs have incorporated immune organs such as the lymph node (LN), limiting their applicability to critical events such as vaccination. Here we developed a 3D-printed, user-friendly device and companion tubing-free impeller pump to co-culture two or more tissue samples, including a LN, under a recirculating common media. Native tissue structure and immune function were incorporated by maintaining slices of murine LN tissue ex vivo in 3D- printed mesh supports for at least 24 hr. In a two-compartment model of a LN and an upstream injection site, vaccination of the multi-tissue chip was similar to in vivo vaccination in terms of locations of antigen accumulation and acute changes in activation markers and gene expression in the LN. We anticipate that in the future, this flexible platform will enable models of multi-organ immune responses throughout the body.

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Gravity and lymphodynamics

Cited by 8 publications

References 29 publications

The transport function of the human lymphatic system—A systematic review

The transport function of the human lymphatic system—A systematic review

Octreotide improves human lymphatic fluid transport a translational trial

A 3D-printed multi-compartment organ-on-chip platform with a tubing-free pump models communication with the lymph node

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