Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum

Dubey, J. P.; Jenkins, Mark C.; Rajendran, C.; Miska, Katarzyna B.; Ferreira, L. R.; Martins, J.; Kwok, O. C. H.; Choudhary, Shanti

doi:10.1016/j.vetpar.2011.05.018

Cited by 166 publications

(94 citation statements)

References 29 publications

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“…Neospora caninum is a protozoan parasite of animals that may have been misdiagnosed as Toxoplasma gondii until 1988 when it was recognized as a distinct Species (DUBEY et al, 2007(DUBEY et al, , 2011. Dogs (Canis lupus familiaris), coyotes (Canis latrans), dingoes (Canis lupus dingo), and gray wolfs (C. lupus) are considered as definitive hosts for this parasite (DUBEY et al, 2011;GONDIM et al, 2004;KING et al, 2010;McALLISTER et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Dogs (Canis lupus familiaris), coyotes (Canis latrans), dingoes (Canis lupus dingo), and gray wolfs (C. lupus) are considered as definitive hosts for this parasite (DUBEY et al, 2011;GONDIM et al, 2004;KING et al, 2010;McALLISTER et al, 1998). Additionally, several livestock and wild animal species have been identified as intermediate hosts of N. caninum (DUBEY & LINDSAY, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Macedo

Miura

et al. 2017

Rev. Bras. Parasitol. Vet.

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The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.Keywords: Neosporosis, fetuses, lesion, serology, cattle. ResumoO objetivo deste estudo foi avaliar a ocorrência de N. caninum associado a abortamentos em vacas de leite do estado de Santa Catarina, sul do Brasil pelo uso das técnicas de ELISA (HerdCheck, IDEXX), reação em cadeia pela polimerase (PCR) e imunohistoquímica (IHC). O sangue das vacas leiteiras que abortaram, bem como, o líquido intratorácico e amostras de tecidos (cérebro, coração, fígado e pulmão) de seus fetos foram coletados e usados para sorologia, PCR (Np21+ e Np6+), e IHC. Vinte e uma vacas (51,2%) de um total de 41, bem como, oito fetos (26,7%) de um total de 30 foram positivos no ELISA (IDEXX) para N. caninum. As vacas > 36 meses de idade tiveram um maior risco de serem soropositivas do que os animais mais jovens. PCR e IHC revelaram que 38,8% e 25,0% dos fetos foram positivos para N. caninum, respectivamente para cada um dos testes. As vacas soropositivas tiveram uma maior frequência de fetos que também foram positivos no fluído intratorácico, na PCR ou na IHC. Em resumo, o presente estudo observou uma alta frequência de N. caninum em abortos de vacas leiteiras na região estudada, com maior prevalência de N. caninum em vacas com mais de 36 meses de idade. Além disso, sorologia, PCR e IHC deveriam ser utilizadas conjuntamente para melhor diagnóstico de neosporose em bovinos.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Macedo

Miura

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…They have similar life-cycles with different definitive hosts, being domestic and free-ranging felids (Frenkel et al 1970) for Toxoplasma and dogs, coyotes and gray wolf (McAllister et al 1998, Gondim et al 2004, Dubey et al 2011 for Neospora. Common intermediate hosts including a wide range of mammals are reported (Dubey 1999, Tenter et al 2000.…”

Section: Introductionmentioning

confidence: 99%

Occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep from four districts of Tocantins state, Brazilian Legal Amazon Region

et al. 2015

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“…Neospora caninum is a protozoan parasite of animals that have domestic dogs, dingo (Canis familiaris), coyote (Canis latrans) and grey wolf (Canis lupus) as its definitive hosts and many species of mammals and birds as intermediate hosts (MCALLISTER et al, 1998;KING et al, 2010;DUBEY et al, 2011). In Brazil, the prevalence of antibodies in dogs from Alagoas, Bahia, Paraíba, Piauí, Pernambuco, Maranhão, Amazonas, Pará, Rondônia, Goiás, Mato Grosso, Mato Grosso do Sul, Minas Gerais, Paraná and São Paulo showed occurrence values that ranged from 2.6% to 67.6% (revised by DUBEY, 2013).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of antibodies anti-Toxoplasma gondii and anti-Neospora caninum in dogs from Natal, RN, Brazil

Lopes

Hernández

Lima

et al. 2015

Braz. J. Vet. Res. Anim. Sci.

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The occurrence of anti-Toxoplasma gondii and Neospora caninum in dogs from the municipality of Natal, RN, Brazil, was determined. Information about the presence of these coccidia in this species was not known. Blood samples were collected from 29 domiciled dogs which inhabit areas that surround two important protected areas of Atlantic Forest (Parque da Cidade and Parque das Dunas) and another 73 dogs that were sacrificed due to Leishmania spp. infection, in Center for Control of Zoonosis (CCZ) for the municipality of Natal. It was only possible to obtain information about the gender of dogs that live in the parks area. The presence of antibodies against each parasite was determined by indirect Fluorescence Antibody Test (IFAT), with a cutoff of 16 for T. gondii and 50 for N. caninum. Of the 102 dogs examined, 13 (12.7%, 95% CI 7.0-20.8%) were T. gondii positive and three (2.9%, CI 0.6-8.4%) for N. caninum. Association between: localities of obtaining samples (parks x CCZ) and sex of animals, with the occurrence of antibodies against each of the parasites, was determined by the Fisher exact test. For T. gondii association was found with males (p = 0.027) and dogs living close to parks (p = 0.008) had higher rates of infection. Associations were not observed in relation to N. caninum.Keywords: Dogs. Toxoplasma gondii. Neospora caninum. Serology. Natal. ResumoA ocorrência de anticorpos anti-Toxoplasma gondii e anti-Neospora caninum foi determinada em cães do município de Natal, RN, onde informações sobre a presença destes coccídios nessa espécie não era conhecida. Para tanto, foram utilizados 29 cães domiciliados, que habitam áreas que circundam duas importantes Unidades de Conservação de Mata Atlântica presentes no município (Parque da Cidade e Parque das Dunas), e outros 73 cães que foram sacrificados no Centro de Controle de Zoonoses de Natal por serem positivos a Leishmania spp. Somente em relação aos cães que vivem próximos aos parques foi possível a obtenção de informações sobre o sexo dos animais. A presença de anticorpos contra cada um dos coccídios foi determinada com a Reação de Imunofluorescência Indireta com ponto de corte de 16 para T. gondii e 50 para N. caninum. Dos 102 cães examinados, 13 (12,7%, IC 95% 7,8) foram positivos para T. gondii e 3 (2,9%, IC 0,6-8,4%) para N. caninum. Associação entre: localidades de obtenção das amostras (parques x CCZ) e sexo dos animais com a ocorrência de anticorpos contra cada um dos parasitos, determinada através do teste exato de Fisher, foram positivas para T. gondii, com os machos (p = 0,027) e cães que habitam próximos aos parques (p = 0,008) apresentando maiores taxas de infecção. Associações não foram observadas em relação a N. caninum.

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Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum

Cited by 166 publications

References 29 publications

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep from four districts of Tocantins state, Brazilian Legal Amazon Region

Occurrence of antibodies anti-Toxoplasma gondii and anti-Neospora caninum in dogs from Natal, RN, Brazil

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