Grazing-incidence diffraction reveals cellulose and pectin organization in hydrated plant primary cell wall

Mundo, Joshua T. Del; Rongpipi, Sintu; Yang, Hui; Ye, Dan; Kiemle, Sarah N.; Moffitt, Stephanie L.; Troxel, Charles; Toney, Michael F.; Zhu, Chenhui; Kubicki, James D.; Cosgrove, Daniel J.; Gomez, Esther W.; Gomez, Enrique D.

doi:10.1038/s41598-023-32505-8

Cited by 10 publications

(6 citation statements)

References 68 publications

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“…We speculate that the 7.4 nm distance would not be very different in the hydrated state as we have previously reported that pectin spacings along the plane of the cell wall do not collapse after drying in the onion cell wall. [ 30 ] Pectin has a persistence length on the scale of 10 nm, [ 31 ] so it is likely to have a stiff conformation. Along with CMFs, pectin is also known to reorient towards the stretch direction.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Structural Change of Plant Epidermal Cell Walls under Strain

Yu,

Del Mundo,

Freychet

et al. 2024

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The molecular foundations of epidermal cell wall mechanics are critical for understanding structure–function relationships of primary cell walls in plants and facilitating the design of bioinspired materials. To uncover the molecular mechanisms regulating the high extensibility and strength of the cell wall, the onion epidermal wall is stretched uniaxially to various strains and cell wall structures from mesoscale to atomic scale are characterized. Upon longitudinal stretching to high strain, epidermal walls contract in the transverse direction, resulting in a reduced area. Atomic force microscopy shows that cellulose microfibrils exhibit orientation‐dependent rearrangements at high strains: longitudinal microfibrils are straightened out and become highly ordered, while transverse microfibrils curve and kink. Small‐angle X‐ray scattering detects a 7.4 nm spacing aligned along the stretch direction at high strain, which is attributed to distances between individual cellulose microfibrils. Furthermore, wide‐angle X‐ray scattering reveals a widening of (004) lattice spacing and contraction of (200) lattice spacing in longitudinally aligned cellulose microfibrils at high strain, which implies longitudinal stretching of the cellulose crystal. These findings provide molecular insights into the ability of the wall to bear additional load after yielding: the aggregation of longitudinal microfibrils impedes sliding and enables further stretching of the cellulose to bear increased loads.

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Section: Discussionmentioning

confidence: 99%

Dynamic Structural Change of Plant Epidermal Cell Walls under Strain

Yu,

Del Mundo,

Freychet

et al. 2024

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“…nanocellulosic materials due to its crystalline nature [73]. The additional diffraction peaks observed throughout the 90° 2θ range indicate the presence of other crystalline constituents such as silica, a known component of rice straw.…”

Section: Characterisation Of Cellulose Fibresmentioning

confidence: 98%

“…00-050-2241). Cellulose Iβ is the most dominant allomorph in higher plants and is frequently targeted for the production of high-quality nanocellulosic materials due to its crystalline nature [73]. The additional diffraction peaks observed throughout the 90 • 2θ range indicate the presence of other crystalline constituents such as silica, a known component of rice straw.…”

Section: Characterisation Of Cellulose Fibresmentioning

confidence: 99%

Steam Explosion-Based Method for the Extraction of Cellulose and Lignin from Rice Straw Waste

Serrano-Martínez,

Pérez-Aguilar,

Carbonell-Blasco

et al. 2024

Applied Sciences

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This paper focuses on the optimisation of an efficient extraction process for cellulose and lignin from rice straw waste from the Albufera of Valencia using the steam explosion method. This method is particularly pertinent given the environmental and economic challenges posed by the current disposal practices of agricultural waste. The technique comprises a high-temperature cooking stage followed by instantaneous decompression, effectively altering the biomass’s physical and chemical properties to enhance its surface area and porosity. Our adaptation of the steam explosion technique specifically addresses the challenges of rice straw waste, marking a significant departure from previous applications. This innovation is crucial in addressing the urgent need for more sustainable waste management practices, as it effectively deconstructs the lignocellulosic matrix of rice straw. This facilitates the selective extraction of cellulose at a 70% efficiency, with a 20% yield and the subsequent recovery of lignin. The results of this study are significant for sustainable biomaterial production, offering novel insights into optimising these crucial biomass components. By refining the process and focusing on critical parameters, our work advances the application of steam explosion methods for agricultural waste, enhancing efficiency and sustainability. By utilising rice straw biowaste, this research not only proposes a solution to a pressing environmental issue but also demonstrates the potential to create new market opportunities, increase the economic value for rice producers, and significantly reduce the environmental footprint of existing waste disposal methods. The holistic and ecological approach of this study underscores the vital need for innovative strategies in agricultural waste management, positioning the valorisation of rice straw waste as a key component in the pursuit of environmental sustainability.

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“…The CI of the MFC and MFC/clay films slightly increased after heat treatment, presumably owing to the increase of the cellulose crystallites size. This was checked using the Debye-Scherrer equation [46]:…”

Section: Structure and Morphology Analysis Of Mfc-clay Composite Filmsmentioning

confidence: 99%

Fluorine Free Surface Modification of Microfibrillated Cellulose-Clay Composite Films: Effect of Hydrophobicity on Gas Barrier Performance

Poothanari,

Leterrier

2024

Surfaces

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Diffusion barrier composite films based on microfibrillated cellulose (MFC) and clay were developed with attention paid to the influence of thermal annealing and a fluorine-free surface silylation on their microstructure, water contact angle (WCA), mechanical properties, oxygen transmission rate (OTR), and water vapor transmission rate (WVTR). The OTR of MFC at 23 °C increased from 1.2 to 25.3 cm3/m2/day/bar as relative humidity increased from 50% to 80%. Annealing increased the film’s crystallinity, surface roughness, and hydrophobicity, while decreasing its OTR by 20% at 80%RH. The addition of clay led to a 30% decrease of OTR at 80%RH due to partial exfoliation and to a 50% decrease when combined with annealing. Silylation increased the hydrophobicity of surface of the film and its combination with clay and annealing led to a WCA of 146.5°. The combination of clay, annealing, and silylation considerably reduced the OTR at 80%RH to a value of 8 cm3/m2/day/bar, and the WVTR at 23 °C and 50%RH from 49 g/m2/day for MFC to 22 g/m2/day. The reduction of OTR and WVTR was found to correlate with the increase in surface hydrophobicity of the film, which was attributed to the reduced access of water molecules within the MFC network.

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Grazing-incidence diffraction reveals cellulose and pectin organization in hydrated plant primary cell wall

Cited by 10 publications

References 68 publications

Dynamic Structural Change of Plant Epidermal Cell Walls under Strain

Dynamic Structural Change of Plant Epidermal Cell Walls under Strain

Steam Explosion-Based Method for the Extraction of Cellulose and Lignin from Rice Straw Waste

Fluorine Free Surface Modification of Microfibrillated Cellulose-Clay Composite Films: Effect of Hydrophobicity on Gas Barrier Performance

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