Green and Cost-Effective Extraction Techniques of Quercetin from Mixture of Nutraceuticals with Yield Analysis via Spectrophotometry and High-Performance Liquid Chromatography Methods

Mahmoud, Mohamed; Abd-ElSalam, Heba-Alla H.; Naguib, Ibrahim A.; Al‐Ghobashy, Medhat A.; Zaazaa, Hala E.; Abdelkawy, Mohamed

doi:10.1093/jaoacint/qsab071

Cited by 8 publications

(4 citation statements)

References 35 publications

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“…A previously reported HPLC method of analysis [35,36] was optimized and validated according to ICH guidelines [37] to evaluate the efficiency of the extraction protocols of GB from sachets. Gradient elution was adopted, using mobile phases consisting of 0.1% orthophosphoric acid of pH 3.00 (mobile phase A) and acetonitrile (mobile phase B).…”

Section: Hplc Chromatographic Methodsmentioning

confidence: 99%

Development of Green and Efficient Extraction Methods of Quercetin from Red Onion Scales Wastes Using Factorial Design for Method Optimization: A Comparative Study

et al. 2021

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Waste resulting from edible plants is considered one of the best sources of valuable phytochemicals. A promising approach for using these appreciated wastes is extracting precious medically important constituents, for example, free quercetin. Two new cost-effective and green extraction methods are introduced in the present study: ultrasound-assisted glycerol extraction (UAGE) and microwave-assisted extraction (MAE). These extraction protocols are optimized using factorial design to define the highest yield of extraction, and HPLC-UV at 370 nm was used as a method of yield analysis. Quercetin remained stable during the whole process in both extraction protocols. A standard addition technique was performed to quantify quercetin in different extracts and eliminate the matrix effect. In UAGE and MAE, extraction yields were 16.55 ± 0.81 and 27.20 ± 1.55 mg/1g from red onion scales on a dry base, respectively. The amount of quercetin extracted using MAE was superior to UAGE in terms of time and yield. A greenness assessment of the offered studies compared to previously published relevant extraction methods was performed using the analytical eco-scale assessment method (ESA) and national environmental methods index (NEMI). MAE showed to be a greener method with a higher ESA score and a greener NEMI pictogram.

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Section: Hplc Chromatographic Methodsmentioning

confidence: 99%

Development of Green and Efficient Extraction Methods of Quercetin from Red Onion Scales Wastes Using Factorial Design for Method Optimization: A Comparative Study

et al. 2021

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“…Test tablets are considered bioequivalent to Dostinex tablets and could be approved as a good substitute for the generic product. Estimation for the greenness of the proposed LC/MS/MS method AGREE approach is simple and reliable for the assessment of eco-friendly characters of the analytical method [19,21,[36][37][38][39][40][41][42][43]. The final greenness numerical value was 0.63 with a relatively pale green color inside the pictogram as demonstrated in Additional file 1: Fig.…”

Section: Results Of Pharmacokinetic Parametersmentioning

confidence: 96%

A validated LC–MS/MS method for analysis of Cabergoline in human plasma with its implementation in a bioequivalent study: investigation of method greenness

et al. 2022

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Cabergoline (CAB) is effective prolactin lowering drug. Evaluation of the bioequivalence for the new test product (0.5 mg CAB film-coated tablets) in Egypt is strongly needed for approval of the drug by the official health authority. Therefore, a highly sensitive and rapid (LC–MS/MS) method was validated for CAB analysis in human plasma. CAB was extracted from plasma via diethyl ether using Quetiapine (QUE) as an internal standard. Multiple reaction monitoring (MRM) in positive ion mode was used, m/z 452.3 → 381.2 for CAB and 384.2 → 253.1 for QUE. Separation was accomplished on a reversed-phase C18. FDA procedures for the bio-analytical method were followed. The method was used in the bioequivalence study to compare the test product (0.5 mg CAB) versus Dostinex tablets, on 24 healthy Egyptian volunteers. The total analysis time was 5.5 min for each sample which permits analysis of various samples per day. The linearity range was from 2.00 to 200.00 pg/mL for CAB. LOD and LOQ were found to be 0.5 and 1.6 pg/mL, respectively. The final greenness numerical value was 0.63 using AGREE tool. The results of pharmacokinetic parameter Tmax were 2.17, and 2.33 h; for test and reference products, respectively. The generic formulation of test product is considered bioequivalent to the reference product Dostinex 0.5 mg tablets and satisfies the requirements of the Egyptian market. The merits of the method over the previous published methods are low cost; availability of cheap internal standard; rapidness; use of acetonitrile-free solvents mobile phase.

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“…Thus, monitoring the amount of quercetin in these foods is important as a contribution to a healthier diet. Quercetin has been quantified by methods involving chromatography [2] and electrochemistry [3]. However, these methodologies can be expensive, time-consuming and require prior knowledge of equipment operation and data processing.…”

Section: Introductionmentioning

confidence: 99%

Development of a quercetin fluorescent sensor prepared from waste paper

Kunst¹,

Passos²,

Paim³

2023

JEOPC

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Green and Cost-Effective Extraction Techniques of Quercetin from Mixture of Nutraceuticals with Yield Analysis via Spectrophotometry and High-Performance Liquid Chromatography Methods

Cited by 8 publications

References 35 publications

Development of Green and Efficient Extraction Methods of Quercetin from Red Onion Scales Wastes Using Factorial Design for Method Optimization: A Comparative Study

Development of Green and Efficient Extraction Methods of Quercetin from Red Onion Scales Wastes Using Factorial Design for Method Optimization: A Comparative Study

A validated LC–MS/MS method for analysis of Cabergoline in human plasma with its implementation in a bioequivalent study: investigation of method greenness

Development of a quercetin fluorescent sensor prepared from waste paper

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