Green Approach to Develop Bee Pollen-Loaded Alginate Based Nanofibrous Mat

The fabrication of skin-care products with therapeutic properties has been significant for human health trends. In this study, we developed efficient hydrophilic composite nanofibers (NFs) loaded with the folic acid (FA) by electrospinning and electrospraying processes for tissue engineering or wound healing cosmetic applications. The morphological, chemical and thermal characteristics, in vitro release properties, and cytocompatibility of the resulting composite fibers with the same amount of folic acid were analyzed. The SEM micrographs indicate that the obtained nanofibers were in the nanometer range, with an average fiber diameter of 75–270 nm and a good porosity ratio (34–55%). The TGA curves show that FA inhibits the degradation of the polymer and acts as an antioxidant at high temperatures. More physical interaction between FA and matrices has been shown to occur in the electrospray process than in the electrospinning process. A UV-Vis in vitro study of FA-loaded electrospun fibers for 8 h in artificial acidic (pH 5.44) and alkaline (pH 8.04) sweat solutions exhibited a rapid release of FA-loaded electrospun fibers, showing the effect of polymer matrix–FA interactions and fabrication processes on their release from the nanofibers. PVA-CHi/FA webs have the highest release value, with 95.2% in alkaline media. In acidic media, the highest release (92%) occurred on the PVA-Gel–CHi/sFA sample, and this followed first-order and Korsmeyer–Peppas kinetic models. Further, the L929 cytocompatibility assay results pointed out that all NFs (with/without FA) generated had no cell toxicity; on the contrary, the FA in the fibers facilitates cell growth. Therefore, the nanofibers are a potential candidate material in skin-care and tissue engineering applications.

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“…The peaks at 1731 cm −1 are typical of carboxylic acid dimers. The results are consistent with the literature [ 40 ].…”

Section: Resultssupporting

confidence: 93%

Fabrication and Characterization of Electrospun Folic Acid/Hybrid Fibers: In Vitro Controlled Release Study and Cytocompatibility Assays

et al. 2021

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“…It comprises proteins, carbohydrates, lipids and amino acids under biologically acceptable conditions ( Anderson et al, 2014 ; Mohammad et al, 2021 ). There has not been any research on the development of bee pollen-loaded alginate-based nanomaterials or any type of polymeric nanofibers until Pakolpakçıl and Draczynski (in 2021) have hypothesised that combining bee pollen with alginate to create green nanofibrous materials might be a viable bioengineering option ( Pakolpakçıl and Draczynski, 2021 ). They used an electrospinning process to create Polish bee pollen-loaded alginate-based nanofibrous mats and studied their shape, chemical content and thermal characteristics.…”

Section: Alginate-based Nanomaterials For Biomedical and Pharmaceutic...mentioning

confidence: 99%

“…The authors indicated that the approach for producing bee pollen-loaded alginate-based nanofibrous material using green electrospinning of water-based systems might be an alternative. They showed how ecologically friendly nanofibrous materials might be created for a variety of uses, including biomedical applications, such as wound dressings and scaffoldings ( Pakolpakçıl and Draczynski, 2021 ).…”

Section: Alginate-based Nanomaterials For Biomedical and Pharmaceutic...mentioning

confidence: 99%

Applications of Alginate-Based Nanomaterials in Enhancing the Therapeutic Effects of Bee Products

Al-Hatamleh

Alshaer

Hatmal

et al. 2022

Front. Mol. Biosci.

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Since the ancient times, bee products (i.e., honey, propolis, pollen, bee venom, bee bread, and royal jelly) have been considered as natural remedies with therapeutic effects against a number of diseases. The therapeutic pleiotropy of bee products is due to their diverse composition and chemical properties, which is independent on the bee species. This has encouraged researchers to extensively study the therapeutic potentials of these products, especially honey. On the other hand, amid the unprecedented growth in nanotechnology research and applications, nanomaterials with various characteristics have been utilized to improve the therapeutic efficiency of these products. Towards keeping the bee products as natural and non-toxic therapeutics, the green synthesis of nanocarriers loaded with these products or their extracts has received a special attention. Alginate is a naturally produced biopolymer derived from brown algae, the desirable properties of which include biodegradability, biocompatibility, non-toxicity and non-immunogenicity. This review presents an overview of alginates, including their properties, nanoformulations, and pharmaceutical applications, placing a particular emphasis on their applications for the enhancement of the therapeutic effects of bee products. Despite the paucity of studies on fabrication of alginate-based nanomaterials loaded with bee products or their extracts, recent advances in the area of utilizing alginate-based nanomaterials and other types of materials to enhance the therapeutic potentials of bee products are summarized in this work. As the most widespread and well-studied bee products, honey and propolis have garnered a special interest; combining them with alginate-based nanomaterials has led to promising findings, especially for wound healing and skin tissue engineering. Furthermore, future directions are proposed and discussed to encourage researchers to develop alginate-based stingless bee product nanomedicines, and to help in selecting suitable methods for devising nanoformulations based on multi-criteria decision making models. Also, the commercialization prospects of nanocomposites based on alginates and bee products are discussed. In conclusion, preserving original characteristics of the bee products is a critical challenge in developing nano-carrier systems. Alginate-based nanomaterials are well suited for this task because they can be fabricated without the use of harsh conditions, such as shear force and freeze-drying, which are often used for other nano-carriers. Further, conjunction of alginates with natural polymers such as honey does not only combine the medicinal properties of alginates and honey, but it could also enhance the mechanical properties and cell adhesion capacity of alginates.

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“…Natural bioactive agents have been used in biomedical due to their significant contribution since ancient times. Lately, the use of natural compounds such as plant extracts [21][22][23], bee products [24][25][26], and oils [27][28][29] for developing electrospun fibrous materials in biomedical applications has been studied extensively. Hypericum perforatum, a member of the Hypericaceae family, has long been regarded as a valuable herbal medicine.…”

Section: Introductionmentioning

confidence: 99%

An In Vitro Study of Antibacterial Properties of Electrospun Hypericum perforatum Oil-Loaded Poly(lactic Acid) Nonwovens for Potential Biomedical Applications

et al. 2021

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The growth of population and increase in diseases that cause an enormous demand for biomedical material consumption is a pointer to the pressing need to develop new sustainable biomaterials. Electrospun materials derived from green polymers have gained popularity in recent years for biomedical applications such as tissue engineering, wound dressings, and drug delivery. Among the various bioengineering materials used in the synthesis of a biodegradable polymer, poly(lactic acid) (PLA) has received the most attention from researchers. Hypericum perforatum oil (HPO) has antimicrobial activity against a variety of bacteria. This study aimed to investigate the development of an antibacterial sustainable material based on PLA by incorporating HPO via a simple, low-cost electrospinning method. Chemical, morphological, thermal, thickness and, air permeability properties, and in vitro antibacterial activity of the electrospun nonwoven fabric were investigated. Scanning electron microscopy (SEM) was used to examine the morphology of the electrospun nonwoven fabric, which had bead-free morphology ultrafine fibers. Antibacterial tests revealed that the Hypericum perforatum oil-loaded poly(lactic acid) nonwoven fabrics obtained had high antibacterial efficiency against Escherichia coli and Staphylococcus aureus, indicating a strong potential for use in biomedical applications.

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Green Approach to Develop Bee Pollen-Loaded Alginate Based Nanofibrous Mat

Cited by 10 publications

References 51 publications

Fabrication and Characterization of Electrospun Folic Acid/Hybrid Fibers: In Vitro Controlled Release Study and Cytocompatibility Assays

Fabrication and Characterization of Electrospun Folic Acid/Hybrid Fibers: In Vitro Controlled Release Study and Cytocompatibility Assays

Applications of Alginate-Based Nanomaterials in Enhancing the Therapeutic Effects of Bee Products

An In Vitro Study of Antibacterial Properties of Electrospun Hypericum perforatum Oil-Loaded Poly(lactic Acid) Nonwovens for Potential Biomedical Applications

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