Green coffee methanolic extract and silymarin protect against CCl4-induced hepatotoxicity in albino male rats

Abstract

Background During the last few decades, patients worldwide have been interested in using alternative medicine in treating diseases to avoid the increased side effects of chemical medications. Green coffee is unroasted coffee seeds that have higher amounts of chlorogenic acid compared to roasted coffee. Green coffee was successfully used to protect against obesity, Alzheimer disease, high blood pressure and bacterial infection. Methods This study aimed to investigate the probable protective activity of the green coffee methanolic extract, silymarin and their combination on CCl4-induced liver toxicity in male rats. Thirty Sprague – Dawley male albino rats were divided into 5 groups; control negative (G1) just got the vehicle (olive oil) and the other four groups received CCl4 dissolved in olive oil through an intraperitoneal injection and were divided into untreated control positive group (G2), the third group (G3) was treated with green coffee methanolic extract, the fourth group (G4) was treated with silymarin, and the fifth group (G5) was treated with a combination of green coffee methanolic extract and silymarin. Results In the positive control group treated with CCl4 (G2), the CCl4-induced toxicity increased lipid peroxidation, IL-6, kidney function parameters, liver function enzymes, total cholesterol, triglycerides and low-density lipoproteins, and decreased irisin, antioxidants, CYP450 and high-density lipoprotein levels. Hepatic tissues were also injured. However, treating the injured rats in G3, G4 and G5 significantly improved the altered parameters and hepatic tissues. Conclusions Green coffee methanolic extract, silymarin, and their combination succeeded in protecting the male rats against CCl4 hepatotoxicity due to their antioxidant activity. Effect of green coffee methanolic extract mixed with silymarin in G5 was more efficient than that of green coffee methanolic extract in G3 or silymarin in G4.

Comparison between the Antioxidant and Antidiabetic Activity of Fenugreek and Buckthorn in Streptozotocin-Induced Diabetic Male Rats

“…Numerous patients lean toward treatments using herbs and depend on traditional medicine to keep away from the unsafe side effects brought about by using synthetic medicines. Medicinal plants contain an assortment of natural products that show considerable antioxidant, antidiabetic, anticancer, and anti-hypercholesterolemic activity [ 16 , 27 , 45 , 46 ].…”

Alleviation of Malathion Toxicity Effect by Coffea arabica L. Oil and Olea europaea L. Oil on Lipid Profile: Physiological and In Silico Study

“…The protective effects of Coffea arabica L. oil against several chemical toxicants linked with the disruption of oxidative damage caused in investigated animals were reported by earlier research studies [61][62][63]. Green coffee causes a clear reduction of cholesterol, triglycerides, VLDL-c, LDL-c and elevates the production of HDL-c [64].…”

The potential role of FNDC5/irisin in various liver diseases: awakening the sleeping beauties

“…reported CCl 4 toxicity promotes inflammatory reaction at aggravated site of liver damage and potentiates oxidative stress (Ref. 89). However, green coffee methanolic extract exerted hepatoprotection by improving irisin and other antioxidants to reverse liver dysfunction, and converting inflammatory cytokines/oxidative stress indices to their normal values in the exposed rats.…”