Green fitted second derivative synchronous spectrofluorometric method for simultaneous determination of remdesivir and apixaban at Nano gram scale in the spiked human plasma

Batubara, Afnan S.; Abdelazim, Ahmed H.; Gamal, Mohammed; Almrasy, Ahmed A.; Ramzy, Sherif

doi:10.1016/j.saa.2022.122265

Cited by 8 publications

(6 citation statements)

References 40 publications

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“…The LLOQ concentration meets the requirements if the analyte response produced is 5 times the blank response and the accuracy and precision value produced is 20%. If the test does not meet the requirements, then the test is carried out at a higher concentration ( 17 ).…”

Section: Methodsmentioning

confidence: 99%

“…The acceptance criteria is ±15% of the nominal concentration unless the LLOQ must be ±20%. In addition, at least 75% of the concentration in the calibration curve must meet these criteria ( 17 ). The area data from the calibration curve are recorded and the linear regression equation is calculated and then the correlation coefficient is obtained.…”

Section: Methodsmentioning

confidence: 99%

“…According to Imam et al (2023), nirmatrelvir plus ritonavir, the similar drugs, can be analyzed using HPLC, but it needs more time to analyze and has higher lowest concentration range ( 16 ). Also, the analysis of remdesivir has been done by Batubara et al (2023) using spectrofluorometric, but the remdesivir needs to be manipulated before analysis ( 17 ). Simultaneously, analysis of favipiravir and remdesivir has also already been done by Ramzy et al (2022) by using spectrofluorometric method, but it also needs a lot of manipulation to overcome the overlap fluorescence spectra of both drugs ( 18 ).…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of method for analysis of favipiravir and remdesivir in volumetric absorptive microsampling with ultra high-performance liquid chromatography–tandem mass spectrophotometry

2023

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Favipiravir and remdesivir are drugs to treat COVID-19. This study aims to find an optimum and validated method for simultaneous analysis of favipiravir and remdesivir in Volumetric Absorptive Microsampling (VAMS) by Ultra High-Performance Liquid Chromatography–Tandem Mass Spectrophotometry. The use of VAMS can be an advantage because the volume of blood is small and the sample preparation process is simple. Sample preparation was done by precipitation of protein using 500 μL of methanol. Analysis was carried out by ultra high-performance liquid chromatography–tandem mass spectrophotometry with ESI+ and MRM with m/z 157.9 > 112.92 for favipiravir, 603.09 > 200.005 for remdesivir, and at m/z 225.968 > 151.991 for acyclovir as the internal standard. The separation was carried out using an Acquity UPLC BEH C18 column (100 × 2.1 mm; 1.7 m), 0.2% formic acid—acetonitrile (50:50), flow rate was 0.15 mL/min, and column temperature was 50°C. The analytical method has been validated with the requirements issued by the Food and Drug Administration (2018) and European Medicine Agency (2011). The calibration range of favipiravir is 0.5–160 μg/mL and 0.002–8 μg/mL for remdesivir.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development and validation of method for analysis of favipiravir and remdesivir in volumetric absorptive microsampling with ultra high-performance liquid chromatography–tandem mass spectrophotometry

2023

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“…The pH 3.1 is achieved by adding orthophosphoric acid to a mixture of acetonitrile, methanol, and water (20:50:30% v/v/v) to create the mobile phase. [18][19][20]…”

Section: Mobile Phase Preparationmentioning

confidence: 99%

New Stability Indicating RP-HPLC Method for Estimation of the Drug Molnupiravir

Deshpande¹,

Shaikh²,

Sable³

et al. 2023

IJPQA

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Background: Molnupiravir was granted approval by the UKS medicines and health product regulatory agency on 04 November 2021 and on 23 December 2021, granted emergency use of authorization by FDA. Objective: Provide a technique for measuring Molnupiravir in active pharmaceutical ingredients and formulations. Method: The wavelength maximum was found to be 236 nm. ICH guidelines were followed. The forced degradation study in the form of acidic, alkali, thermal, photolytic, hydrolytic, and oxidative stress conditions was carried out for Molnupiravir. Results: The method was linear, as measured by a coeffi cient of correlation (R2) of 0.9991 in the 10 to 50 μg/mL range. The %RSD for precision, accuracy, limit of detection (LoD), limit of quantitation (LoQ), ruggedness, and robustness was within acceptable limits per ICH Q2 (R1). Conclusion: HPLC equipped with a UV detector is used to create and verify the proposed method. An acetonitrile mobile phase component of 20% was used, demonstrating the more cost-eff ective technique. The extensive data of mobile phase optimization gives a complete idea of fi nal chromatographic conditions, which can be further implemented for future analysis. Molnupiravir shows less than 4% degradation under diff erent stress conditions. The forced degradation data helps show stability, indicating the behavior of Molnupiravir.

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“…Because the amplitude of the derivative signal has an inverse relationship to the bandwidth of the original spectrum, combining SFS with derivatives allows for sensitivity differentiation of the standard emission spectrum [34]. Derivative synchronous fluorometry (DSF) has been used in recent years to analyze various combinations in coadministered and biological fluid samples [35][36][37][38][39][40].…”

mentioning

confidence: 99%

A novel sustainable second‐derivative synchronous spectrofluorimetric method for the simultaneous determination of olmesartan medoxomil and rosuvastatin calcium in bulk and formulations: Integrated greenness and whiteness evaluation

Batakoushy,

Nasr,

Soliman

et al. 2023

Luminescence

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The present research has established a quick and highly sensitive second‐derivative synchronous fluorometric technique for the simultaneous quantification of a binary mixture of olmesartan medoxomil and rosuvastatin calcium. Simultaneously, the suggested approach was used to detect the synchronous fluorescence intensity of the cited drugs at Δ λ = 80 nm in ethanol to determine the concentrations of olmesartan medoxomil and rosuvastatin calcium at 265 and 240 nm, respectively. Various experimental conditions were tested, and each variable was analyzed and optimized. The calibration graphs were shown to be linear within ranges of 0.1–2.0 and 0.5–6.0 μg ml−1 for each drug concentration, respectively. The newly developed Green Solvents Selecting Tool (GSST) was utilized to assess the solvent's sustainability. Furthermore, the proposed method was found to be environmentally friendly after being evaluated with three different tools [the Green Analytical Procedure Index (GAPI), the Analytical Greenness Metric (AGREE), and the Analytical Eco‐Scale with Eco‐score equal to 95]. The whiteness qualities were also studied using the Red–Green–Blue (RGB12) model, which was recently designed and showed a high score equal to 92.9. The proposed method’s good findings, as well as its ongoing sustainability, simplicity, and economy, stimulate its application in QC laboratories.

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Green fitted second derivative synchronous spectrofluorometric method for simultaneous determination of remdesivir and apixaban at Nano gram scale in the spiked human plasma

Cited by 8 publications

References 40 publications

Development and validation of method for analysis of favipiravir and remdesivir in volumetric absorptive microsampling with ultra high-performance liquid chromatography–tandem mass spectrophotometry

Development and validation of method for analysis of favipiravir and remdesivir in volumetric absorptive microsampling with ultra high-performance liquid chromatography–tandem mass spectrophotometry

New Stability Indicating RP-HPLC Method for Estimation of the Drug Molnupiravir

A novel sustainable second‐derivative synchronous spectrofluorimetric method for the simultaneous determination of olmesartan medoxomil and rosuvastatin calcium in bulk and formulations: Integrated greenness and whiteness evaluation

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