Green fluorescent protein as a visual selection marker for oil palm transformation

Parveez, Ghulam Kadir Ahmad; Majid, Na'imatulapidah Abdul

doi:10.1016/j.indcrop.2018.02.016

Cited by 11 publications

(3 citation statements)

References 60 publications

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“…In plant genetic engineering, a reporter gene is used as a tool to monitor the transfer and expression of foreign genes in the recipient host. The gfp gene was used as the reporter gene because it allows for non-destructive detection without any substrates and chemicals in any species (Parveez and Majid, 2018). The GFP signals were detected from calli transformed with every construct, indicating that the constructs were viable and functioning in oil palm cells (Figure 6).…”

Section: Evaluation Of Transformation Vectors In Oil Palm Callimentioning

confidence: 99%

VECTOR CONSTRUCTION AND TRANSIENT EVALUATION IN OIL PALM CALLI VIA Agrobacterium-MEDIATED TRANSFORMATION

AYUB¹

2023

JOPR

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Current oil palm transformation efficiency was relatively low compared to other monocots such as rice and sorghum. Thus, the construction of efficient transformation vectors is crucial for Agrobacterium-mediated oil palm genetic engineering to elevate the transformation efficiency. In this study, six transformation vectors, which contain phosphinothricin acetyltransferase (bar), modified green fluorescent protein (mgfp) and synthetic green fluorescent protein (sgfp(S65T)) genes driven by either maize ubiquitin (Ubi-1) or cauliflower mosaic virus (CaMV35S) promoters, were assembled in pBINPLUS/ARS or pCAMBIA0380 backbones. The efficiency of these vectors was evaluated in oil palm calli. Based on transient GFP signals, the pBINPLUS/ARS-based vectors produced a higher number of GFP signals than the pCAMBIA0380-based vectors. The CaMV35S promoter was more excellent than the Ubi-1 promoter in driving the expression of gfp genes. Furthermore, the sgfp(S65T) variant was more suitable for oil palm transformation than the mgfp variant because it produced more GFP signals in the oil palm calli than the mgfp variant. These results suggested that the pBAR65 vector, which carries the bar and sgfp(S65T) genes driven by the CaMV35S promoter in the pBINPLUS/ARS backbone, is the most suitable vector and could be used to develop an efficient Agrobacterium-mediated transformation system for oil palm.

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Section: Evaluation Of Transformation Vectors In Oil Palm Callimentioning

confidence: 99%

VECTOR CONSTRUCTION AND TRANSIENT EVALUATION IN OIL PALM CALLI VIA Agrobacterium-MEDIATED TRANSFORMATION

AYUB¹

2023

JOPR

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“…An internal control gene was amplified by using a programme described by Nurfahisza et al (2014) (data not shown). This is to ensure that the DNA samples were suitable for PCR amplification (Parveez and Majid, 2018). Later, the amplifications of transgenes were carried out by using primers UbibarF1 and BarR2 which would amplify a part of ubiquitin promoter and bar gene.…”

Section: Molecular Analysis Of Putative Transgenic Plantletsmentioning

confidence: 99%

Short Communication: Optimisation of the Bombardment Parameters for Transformation and Regeneration of Stable Oil Palm Transformants

Rahman¹

2020

JOPR

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Genetic transformation approaches by using particle bombardment have been an integral part in efforts to improve palm oil quality. The bombardment parameters were previously evaluated to efficiently deliver deoxyribonucleic acid (DNA) during bombardment. However, the evaluation was carried out at transient stage and therefore the results may not be very precise. In order to improve the DNA delivery and regeneration of the oil palm carrying transgenes, a study at stable stage was performed and discussed in this paper. Oil palm embryogenic calli were bombarded at several parameters using pAHC25 plasmid. Selection on media containing 1 mg litre-1 of bialaphos was initiated after one month of bombardment. Then, the concentration of bialaphos was increased to 2 mg litre-1 and subsequently to 3 mg litre-1 to effectively eliminate the regeneration of untransformed cells. Generated shoots were subjected to polymerase chain reaction (PCR) analysis. The best bombardment condition was determined based on the highest number of shoots carrying the transgene. The bombardment at 1100 psi helium pressure with 7.5 cm distance between stopping plate to tissue and one time bombardment using 2.0 µg DNA per bombardment was obtained as the best condition for DNA delivery and also minimised tissue damage. Hence, the results of this study will be useful as a guideline to generate transformed oil palm.

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“…Selection of true and stable transformants has been challenging for oil palm genetic engineering on a whole. The use of the green fluorescent protein gene (gfp) as a selectable marker for oil palm was attempted (Parveez and Majid, 2018), however it did not result in the regeneration of any stable transformants. In 2015, although Parveez et al successfully synthesised polyhydroxybutyrate (PHB), a form of biodegradable plastic, albeit in a small amount in the oil palm, its true integration was never verified until recently.…”

Section: Advancing With Biotechnologymentioning

confidence: 99%

OIL PALM ECONOMIC PERFORMANCE IN MALAYSIA AND R&D PROGRESS IN 2018

Kushairi¹

2019

JOPR

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Staying resilient is probably an understatement for the oil palm industry during the period of 2018. Whilst other sectors such as the electrical and electronics and other manufactured products were experiencing a bullish growth, the export value of palm oil and palm oil-based products was on the decline. This is essentially due to the looming threat of a total ban of palm oil-based biodiesel by the European Union (EU) come 2021 and the higher production from palm oil plantations in Indonesia causing lower demand and oversupply worldwide. In view of the former, Malaysia's export market scene is expected to change in the coming years. Malaysia has since identified several potential new markets in Africa, Central and South Asia. The negative campaigns relating to health, social or environment targeted at the oil palm are not new but the current action led by the West is clearly causing a major dent in the industry. As the most traded commodity, the industry as a whole is constantly subjected to scrutiny with environment being the current key issue warranting the imposition of checks and balances such as the Malaysian Sustainable Palm Oil (MSPO), Indonesian Sustainable Palm Oil (ISPO) and Rountable Sustainable Palm Oil (RSPO) certification. At the same time, every aspect of the research continues to progress further building on prior knowledge arising directly from working on the oil palm or through model systems. Their progress is briefly highlighted in this review. Clearly the main theme of the research from upstream to midstream and finally downstream are basically aligned with and directed towards achieving the Sustainable Development Goals (SDG) by 2030. This would consequently help further improve the perception of oil palm/palm oil in general.

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Green fluorescent protein as a visual selection marker for oil palm transformation

Cited by 11 publications

References 60 publications

VECTOR CONSTRUCTION AND TRANSIENT EVALUATION IN OIL PALM CALLI VIA Agrobacterium-MEDIATED TRANSFORMATION

VECTOR CONSTRUCTION AND TRANSIENT EVALUATION IN OIL PALM CALLI VIA Agrobacterium-MEDIATED TRANSFORMATION

Short Communication: Optimisation of the Bombardment Parameters for Transformation and Regeneration of Stable Oil Palm Transformants

OIL PALM ECONOMIC PERFORMANCE IN MALAYSIA AND R&D PROGRESS IN 2018

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