Green peas protein hydrolyzed by bromelain in simple procedure to improve kidney function in cisplatin-induced rats

Bromelain a Potential Bioactive Compound: A Comprehensive Overview from a Pharmacological Perspective

“…Bromelain from the juice of cooled pineapple undergoes various processes, including ultrafiltration, centrifugation, and lyophilization, which results in the production of a yellow powder. The proteolytic activity of bromelain is conducted by a group of substrates, including gelatin, chromogenic tripeptides, and casein [ 26 , 27 ], and results in several amino acids—tyrosine, alanine, and lysine [ 28 ]—from the digestion of proteins. Bromelain has also been found to be useful in the hydrolysis of mackerel [ 29 ].…”

Recent Advances in In Vitro and In Vivo Studies of Antioxidant, ACE-Inhibitory and Anti-Inflammatory Peptides from Legume Protein Hydrolysates

“…Chronic kidney disease (CKD) is a condition that can lead to kidney failure, cardiovascular disease (CVD), and death [ 118 ]. It is a leading cause of morbidity and mortality worldwide, affecting 600 million people, and is characterized by a gradual loss of kidney function [ 119 , 120 ].…”

“…Based on this, dietary protein restriction is a successful strategy for reducing CKD signs and symptoms, although it is undesirable to many patients and can lead to protein malnutrition [ 125 ]. Therefore, the bioactive peptides produced by enzymatic hydrolysis of food proteins have been proven to have promising effects on human health and illness, and the synthesis of prospective peptides should be based on the characteristics of critical organ functions, such as the kidneys, in this case [ 118 ]. However, studies examining the impact of plant proteins on renal disease are mostly limited to soy protein from legumes, proposed as a partial replacement or a safe alternative for animal protein-based diets in CKD [ 126 ].…”

Sub-chronic toxicity study of green peas protein hydrolysate in rats

“…Research on the protein hydrolysate of green peas ( Pisum sativum ) which was hydrolyzed using the bromelain enzyme showed effective results in increasing the rats’ kidney function parameters induced by Gentamicin or Cisplatin [13] , [14] . Besides, this hydrolysate is proven to have antioxidant activity, hypolipidemic effects, and has a high content of several essential amino acids so that it is expected to be beneficial for the health of CKD sufferers [13] , [14] , [15] .…”

“…Research on the protein hydrolysate of green peas ( Pisum sativum ) which was hydrolyzed using the bromelain enzyme showed effective results in increasing the rats’ kidney function parameters induced by Gentamicin or Cisplatin [13] , [14] . Besides, this hydrolysate is proven to have antioxidant activity, hypolipidemic effects, and has a high content of several essential amino acids so that it is expected to be beneficial for the health of CKD sufferers [13] , [14] , [15] . With this potential, in the future, this protein hydrolysate of green peas bromelain (PHGPB) has the potential to be used as a supportive treatment for CKD sufferers in long term, so it is necessary to know the safety aspect of this hydrolysate.…”