Green synthesis, characterisation and biological evaluation of AgNPs using Agave americana, Mentha spicata and Mangifera indica aqueous leaves extract

Abstract

The current study was performed to synthesize stable, eco-friendly and bio-compatible silver nano-particles (AgNPs) of , and leaves and to screen them for biological activities. The ultraviolet-visible spectroscopic analysis revealed that λ-max for AgNPs range from 350-500 nm. All AgNPs possessed polycrystalline structure as notified as intense graphical peaks in complete spectrum of 20 values ranging from 10-80° in X-ray diffraction measurements and supported by scanning electron microscopy data. The size of the nano-particles was confirmed by transmission electron microscopy (30-150 nm). Mass loss at variable temperatures was evaluated by simultaneous thermogravimetric and differential thermal analysis revealed reduction in mass and activity of compounds was notified by temperature increase from 200 to 800 °C, thus concluding it as thermally sensitive compounds. AgNPs showed significant (96%) activity against , (95%) and (89%). Good antioxidant activity was shown by AgNPs at 300 µl (79%). AgNPs showed significant phytotoxic activity (88%) at highest concentration. No haemagglutination reaction was observed for the test samples. The above results revealed that AgNPs synthesized from selected plant species possesses significant antimicrobial and phytotoxic effect.

Pinus wallichiana-synthesized silver nanoparticles as biomedical agents: in-vitro and in-vivo approach

“…The diffracted intensities were calculated from 10°to 90°of 2θ angles. A voltage of 20-40 kV was used and measurement was done at a wavelength of 1.5418 Å in θ − 2θ configurations using the Debye-Scherrer equation (11).…”

“…The structure of the synthesized AgNPs was analyzed using a scanning electron microscope (SEM) (JSM5910, JEOL, Japan). Copper grid carbon was coated with a thin film of AgNPs, mixed with distilled water and allowed to dry in the presence of hot air for 5 min and then examined under an electron microscope at various magnification powers (11).…”

“…Elemental analysis of AgNPs was carried out with energy dispersive X-ray (EDX) detection coupled with SEM (JSM-5910 with model INCA-200), Oxford instruments, UK. The AgNPs were analyzed at sticky tape fixed on a microscopic stub of aluminum on both sides (11).…”

“…The plant-derived AgNPs and aqueous extract(s) were analyzed by FTIR spectroscopy (Prestige-21, Shimadzu, Japan) from 4000 to 400 cm −1 to determine the functional groups involved in AgNP synthesis (11).…”

In situ formation of AgNPs on S. cerevisiae surface as bionanocomposites for bacteria killing and heavy metal removal

“…Though many chemical and physical methods could be used to prepare silver nanomaterials, these synthesis methods were characterized by high energy consumption, low efficiency and secondary pollution (Zhang et al 2016). Recently, biosynthesis of silver nanomaterials has been developed as a low-cost, convenient, efficient and environmentally friendly method (Salunke et al 2016;Velusamy et al 2016;Ahmad et al 2016). Microorganisms as nanomaterials biosynthesis factories have the potential to prepare silver nanomaterials.…”