Green synthesized silver nanoparticles destroy multidrug resistant bacteria via reactive oxygen species mediated membrane damage

Das, Balaram; Dash, Sandeep Kumar; Mandal, Debasish; Ghosh, Totan; Chattopadhyay, Sourav; Tripathy, Satyajit; Das, Sabyasachi; Dey, Sumit; Das, Debasis; Roy, Somenath

doi:10.1016/j.arabjc.2015.08.008

Cited by 349 publications

(193 citation statements)

References 47 publications

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“…Silver nanoparticles can be responsible for release of lipopolysaccharides and membrane proteins, collapsing of bacterial membrane potential induced by drop of ATP level by nano-Ag [52, 53]. Researches propose many mechanisms of AgNPs antibacterial activity, but still exact mechanism has not been confirmed and needs further studies.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles

Składanowski

Golińska

Rudnicka

et al. 2016

Med Microbiol Immunol

109

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The study was focused on assessment of antibacterial activity, cytotoxicity and immune compatibility of biogenic silver nanoparticles (AgNPs) synthesized from Streptomyces sp. NH28 strain. Nanoparticles were biosynthesized and characterized by UV–Vis spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, nanoparticle tracking analysis system and zeta potential. Antibacterial activity was tested against Gram-positive and Gram-negative bacteria; minimal inhibitory concentration was recorded. Cytotoxicity was estimated using L929 mouse fibroblasts via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Biocompatibility of AgNPs was performed using THP1-XBlue™ cells. Biogenic AgNPs presented high antibacterial activity against all tested bacteria. Minimum inhibitory concentration of AgNPs against bacterial cells was found to be in range of 1.25–10 μg/mL. Silver nanoparticles did not show any harmful interaction to mouse fibroblast cell line, and no activation of nuclear factor kappa-light-chain-enhancer of activated B (NF-κB) cells was observed at concentration below 10 µg/mL. The half-maximal inhibitory concentration (IC50) value was established at 64.5 μg/mL. Biological synthesis of silver can be used as an effective system for formation of metal nanoparticles. Biosynthesized AgNPs can be used as an antibacterial agent, which can be safe for eukaryotic cells.

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Section: Discussionmentioning

confidence: 99%

Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles

Składanowski

Golińska

Rudnicka

et al. 2016

Med Microbiol Immunol

109

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“…48,49 The uptake of nanoparticles by bacterial cells also leads to the depletion of intracellular adenosine triphosphate production, disruption of DNA replication, and damage. 47 Moreover, the exposure of bacteria to the silver ions released from nanoparticles leads to cellular respiration arrest and renders them in a state defined as "active, but nonculturable" in which they fail to grow. 44,47 The titanium disks containing SNPs provided antibacterial properties against all clinical strains of the bacteria.…”

mentioning

confidence: 99%

“…47 Moreover, the exposure of bacteria to the silver ions released from nanoparticles leads to cellular respiration arrest and renders them in a state defined as "active, but nonculturable" in which they fail to grow. 44,47 The titanium disks containing SNPs provided antibacterial properties against all clinical strains of the bacteria. The toxicity was not related to the duration of silver incorporation, as all the samples treated with Tollens were comparably antibacterial.…”

mentioning

confidence: 99%

“…12,[43][44][45] The accumulation and dissolution of nanoparticles leading to the disruption of the bacterial membrane and release of bacterial intracellular biomolecules have also been discussed. 46,47 Another mechanism is based on the generation of reactive oxygen species and damage to cellular structures, unregulated cell signaling, changes in cell motility, and apoptosis. 48,49 The uptake of nanoparticles by bacterial cells also leads to the depletion of intracellular adenosine triphosphate production, disruption of DNA replication, and damage.…”

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confidence: 99%

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In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery

Pokrowiecki¹,

Zaręba²,

Szaraniec³

et al. 2017

IJN

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The addition of an antibacterial agent to dental implants may provide the opportunity to decrease the percentage of implant failures due to peri-implantitis. For this purpose, in this study, the potential efficacy of nanosilver-doped titanium biomaterials was determined. Titanium disks were incorporated with silver nanoparticles over different time periods by Tollens reaction, which is considered to be an eco-friendly, cheap, and easy-to-perform method. The surface roughness, wettability, and silver release profile of each disc were measured. In addition, the antibacterial activity was also evaluated by using disk diffusion tests for bacteria frequently isolated from the peri-implant biofilm: Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus sanguis, Porphyromonas gingivalis, Staphylococcus aureus , and Escherichia coli . Cytotoxicity was evaluated in vitro in a natural human osteoblasts cell culture. The addition of nanosilver significantly increased the surface roughness and decreased the wettability in a dose-dependent manner. These surfaces were significantly toxic to all the tested bacteria following a 48-hour exposure, regardless of silver doping duration. A concentration of 0.05 ppm was sufficient to inhibit Gram-positive and Gram-negative species, with the latter being significantly more susceptible to silver ions. However, after the exposure of human osteoblasts to 0.1 ppm of silver ions, a significant decrease in cell viability was observed by using ToxiLight™ BioAssay Kit after 72 hours. Data from the present study indicated that the incorporation of nanosilver may influence the surface properties that are important in the implant healing process. The presence of nanosilver on the titanium provides an antibacterial activity related to the bacteria involved in peri-implantitis. Finally, the potential toxicological considerations of nanosilver should further be investigated, as both the antibacterial and cytotoxic properties may be observed at similar concentration ranges.

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“…Biological methods secure safe, cost effective, ecofriendly and easy ways of Ag-NPs formation 25 . Extracts from plants, bacteria and fungi can be used to biosynthesize Ag-NPs by reducing silver salts 26,27 .…”

Section: Discussionmentioning

confidence: 99%

Silver Nanoweapons: A novel Tool against Multidrug Resistant Bacteria

Arafat¹,

El-Sayed²,

Askora³

2016

Biosci., Biotech. Res. Asia

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Antibacterial activities of Ag-NPs have received much attention due to their effective killing and cost-effectiveness. Biosynthesis is an attractive and eco-friendly method to produce silver nanoparticles (Ag-NPs). Ag-NPs are considered a promising tool to overcome the emergence of multi-drug resistant bacteria. In this study, bio-synthesis of Ag-NPs was attempted using plant extracts of peppermint. Characterization of Ag-NPs was achieved by UV-visible spectrophotometer and TEM. Monodispersed Ag-NPs were obtained with different sizes ranged from 6 to 88 nm. Antibacterial activities of Ag-NPs against pathogenic bacteria were evaluated using disc diffusion method. Our results indicated that Gram positive bacteria were more susceptible than Gram negative. The current study offers a cost-effective and eco-friendly method for biosynthesis of potent bactericidal Ag-NPs and their use against human pathogenic bacteria.

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Green synthesized silver nanoparticles destroy multidrug resistant bacteria via reactive oxygen species mediated membrane damage

Cited by 349 publications

References 47 publications

Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles

Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles

In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery

Silver Nanoweapons: A novel Tool against Multidrug Resistant Bacteria

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