Green Synthesized Silver Nanoparticles using Crocus sativus L Extract after reduces Prehepatocellular Carcinoma In Rats

Abstract

I n this study, the effects of green synthesized silver nanoparticles (Ag NPs) using crocus sativus L were investigated on the liver tissues of white albino rats after induced the prehepatocellular carcinoma using diethylnitrosamine. Thirty male albino rats weight (150-200) gm ,were used by dividing them into five groups, each group contains 6 rats. Group 1(control group) was given food and water like other groups by liberty. Group 2 was intraperitoneal injected with single dose of diethylnitrosamine200 mg/kg b. wt . Group 3 was intraperitoneal injected with a AgNPs 200 mg/kg b. wt for six weeks . Group 4 was intraperitoneal injected with single dose of diethylnitrosamine 200 mg/kg b. wt followed by, intraperitoneal injected with a AgNPs 200 mg/kg b. wt for six weeks . Group 5 was intraperitoneally injected with a AgNPs 200 mg/kg b. wt for six weeks ,followed by intraperitoneal injected with single dose of Diethylnitrosamine200 mg/kg b. wt . All animals were sacrified at the end of experiment. The histophathological studies revealed that group 1 have normal hepatocyte. Group2 have sever necrosis, fatty change , atypical cells and bile duct proliferation . Group 3, have normal hepatocyte cell with mild necrosis. Groups 4, have certain area of necrosis , inflammatory cells infiltration ,congestion and mild fatty change, no atypical cells were seen. Group 5 the liver section showing certain area of necrosis , inflammatory cells infiltration ,mild fatty change and no atypical cells.