Green tea extract containing enhanced levels of epimerized catechins attenuates scopolamine-induced memory impairment in mice

Bae, Ho Jung; Kim, Jihyun; Jeon, Se Jin; Kim, Jae-Hoon; Goo, Nayeon; Jeong, Yongwoo; Cho, Kyungnam; Cai, Mudan; Kwon, Kyoung Ja; Ryu, Jong Hoon

doi:10.1016/j.jep.2020.112923

Cited by 22 publications

(14 citation statements)

References 54 publications

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“…Rg1 is not the only drug that can effectively inhibit oxidative stress. The latest research suggests that green tea catechins can also inhibit the generation of ROS, improve spatial memory, and accelerate Aβ degradation [ 47 , 48 ]. However, the expected therapeutic effect has not been achieved in clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rg1 alleviates Aβ deposition by inhibiting NADPH oxidase 2 activation in APP/PS1 mice

Zhang

Sun

et al. 2021

Journal of Ginseng Research

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Background Ginsenoside Rg1 (Rg1), an active ingredient in ginseng, may be a potential agent for the treatment of Alzheimer’s disease (AD). However, the protective effect of Rg1 on neurodegeneration in AD and its mechanism of action are still incompletely understood. Methods Wild type (WT) and APP/PS1 AD mice, from 6 to 9 months old, were used in the experiment. The open field test (OFT) and Morris water maze (MWM) were used to detect behavioral changes. Neuronal damage was assessed by hematoxylin and eosin (H&E) and Nissl staining. Immunofluorescence, western blotting, and quantitative real-time polymerase chain reaction (q-PCR) were used to examine postsynaptic density 95 (PSD95) expression, amyloid beta (Aβ) deposition, Tau and phosphorylated Tau (p-Tau) expression, reactive oxygen species (ROS) production, and NAPDH oxidase 2 (NOX2) expression. Results Rg1 treatment for 12 weeks significantly ameliorated cognitive impairments and neuronal damage and decreased the p-Tau level, amyloid precursor protein (APP) expression, and Aβ generation in APP/PS1 mice. Meanwhile, Rg1 treatment significantly decreased the ROS level and NOX2 expression in the hippocampus and cortex of APP/PS1 mice. Conclusions Rg1 alleviates cognitive impairments, neuronal damage, and reduce Aβ deposition by inhibiting NOX2 activation in APP/PS1 mice.

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Section: Discussionmentioning

confidence: 99%

Ginsenoside Rg1 alleviates Aβ deposition by inhibiting NADPH oxidase 2 activation in APP/PS1 mice

Zhang

Sun

et al. 2021

Journal of Ginseng Research

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“…The same conclusion has been drawn by other studies. Supplementation with whole GTE has restored cognitive impairments in a variety of experimental models including aged ( Li et al., 2009 ), maternal deprived ( Menezes et al., 2017 ), and stroke ( Altermann et al., 2017 ) animals as well as, scopolamine ( Bae et al., 2020 ), ethanol ( Zhang et al., 2018 ), and beta-amyloid ( Haque et al., 2008 ) -treated models. Among the different polyphenolic ingredients of green tea, EGCG has appealed the most attention.…”

Section: Discussionmentioning

confidence: 99%

The beneficial effects of green tea on sleep deprivation-induced cognitive deficits in rats: the involvement of hippocampal antioxidant defense

Forouzanfar

Gholami

Foroughnia

et al. 2021

Heliyon

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“…Heat transformed green tea (HTGT: Lot #. 180426) was produced as described previously [ 16 ]. Briefly, dried leaf of green tea was extracted with 50% ethanol and heated at 115 °C for 5 h to facilitate the conversion of epigallocatechin gallate to gallocatechin gallate (GCG).…”

Section: Methodsmentioning

confidence: 99%

Anti-obesity effects of heat-transformed green tea extract through the activation of adipose tissue thermogenesis

Lee

Kim

et al. 2022

Nutr Metab (Lond)

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Background Adipose tissue thermogenesis is a potential therapeutic target to increase energy expenditure and thereby combat obesity. The aim of the present study was to investigate the thermogenic and anti-obesity effects of heat-transformed green tea extract (HTGT) and enzymatically modified isoquercetin (EMIQ). Methods Immortalized brown pre-adipocytes and C3H10T1/2 cells were used for in vitro analyses. A high-fat diet (HFD)-induced obesity mouse model and CIDEA-reporter mice were used for in vivo experiments. The effects of HTGT and EMIQ on mitochondrial metabolism were evaluated by immunoblot, mitochondrial staining, and oxygen consumption rate analyses. In vivo anti-obesity effects of HTGT and EMIQ were measured using indirect calorimetry, body composition analyses, glucose tolerance tests, and histochemical analyses. Results Co-treatment with HTGT and EMIQ (50 μg/mL each) for 48 h increased brown adipocyte marker and mitochondrial protein levels (UCP1 and COXIV) in brown adipocytes by 2.9-fold, while the maximal and basal oxygen consumption rates increased by 1.57- and 1.39-fold, respectively. Consistently, HTGT and EMIQ treatment increased the fluorescence intensity of mitochondrial staining in C3H10T1/2 adipocytes by 1.68-fold. The combination of HTGT and EMIQ (100 mg/kg each) increased the expression levels of brown adipocyte markers and mitochondrial proteins in adipose tissue. Two weeks of HTGT and EMIQ treatment (100 mg/kg each) led to a loss of 3% body weight and 7.09% of body fat. Furthermore, the treatment increased energy expenditure by 8.95% and improved glucose tolerance in HFD-fed mice. Conclusions The current study demonstrated that HTGT and EMIQ have in vivo anti-obesity effects partly by increasing mitochondrial metabolism in adipocytes. Our findings suggest that a combination of HTGT and EMIQ is a promising therapeutic agent for the treatment of obesity and related metabolic diseases.

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Green tea extract containing enhanced levels of epimerized catechins attenuates scopolamine-induced memory impairment in mice

Cited by 22 publications

References 54 publications

Ginsenoside Rg1 alleviates Aβ deposition by inhibiting NADPH oxidase 2 activation in APP/PS1 mice

Ginsenoside Rg1 alleviates Aβ deposition by inhibiting NADPH oxidase 2 activation in APP/PS1 mice

The beneficial effects of green tea on sleep deprivation-induced cognitive deficits in rats: the involvement of hippocampal antioxidant defense

Anti-obesity effects of heat-transformed green tea extract through the activation of adipose tissue thermogenesis

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