“Green technology” processing of pine (Pinus sylvestris L.) and larch (Larix sibirica Ledeb.) wood greenery to produce bioactive extracts

Abstract

In order to explore the extractives of conifers an effective and environmentally friendly method of extraction with aqueous-alkaline solution allowing to isolate up to 10.4% of extractive substances (ES) from pine wood greenery and up to 6.9% from larch wood greenery was investigated. The component fractional composition of aqueous-alkaline extracts was studied. The antioxidant fraction activity of neutral and acidic components isolated from the produced extracts was evaluated. It was found that these fractions have a high antioxidant activity, where the activity of larch extract components was higher than that of the respective components of pine extract. Due to their rich chemical composition and high biological activity, extracts of pine and larch wood greenery produced by aqueous-alkaline extraction have good application prospects as biologically active preparations.

Novel Mannich Bases of α‐Mangostin Bearing Methoxyphenyl Moieties with Antioxidant and Membrane‐protective activity

“…1 H! 13 C correlations of HÀ C( 5), CH 2 (11), and C(7)À OMe protons with C(7) atom are observed in the HMBC NMR spectrum of the compound 6, which confirms lack of substitution at the C( 5) position (Figure S27).…”

“…We compared the AOA of α‐mangostin ( 1 ) and its derivatives 2 – 6 using different test systems, including cell‐based system, all of which have been successfully used in previous studies [7a,b,d,9,13] . One of the methods used to assess the AOA of chemical compounds and different extracts is oxidative hemolysis of mammalian RBCs.…”

“…The structures of the synthesized derivatives 2-6 were determined by spectral analysis, their composition was confirmed using elemental analysis (see Supporting Information). The 1 H and 13 C NMR spectra of these compounds show proton and carbon signals of the C-4-substituted xanthone skeleton: two prenyl groups at the C-2 and C-8 positions, a methoxyl group at the C-7 position, three hydroxyl groups at the C-6, C-3, and C-1 positions, and an unsubstituted position at the C-5 position. The same spectra show proton and carbon signals characteristic of substituents at the C-21 position: the morpholine fragment and the substituted methoxyphenyl substituent (compounds 2-5), or the methylene group (compound 6).…”

“…The melting points were measured on a Sanyo Gallenkamp MDP350 and were not corrected. The NMR 1 H and 13 C spectra were recorded on a Bruker Avance II 300 instrument (300 MHz and 75 MHz) in [D 6 ]acetone, [D 6 ]DMSO, [D 6 ]DMSO + [D 6 ]acetone, and CDCl 3 + [D 4 ]methanol. The chemical shifts were referenced to the residual signals of acetone, DMSO, and CHCl 3 .…”

“…The chemical shifts were referenced to the residual signals of acetone, DMSO, and CHCl 3 . The signals of carbon atoms were assigned using 13 C NMR spectra in J-modulation mode and literature data for αmangostin; [26] some assignments were made using HSQC and HMBC experiments. NMR spectral data was processed using a MestReNova (ver.…”