Ground level environmental protein concentrations in various ecuadorian environments: Potential uses of aerosolized protein for ecological research

Staton, Sarah J. R.; Woodward, Andrea; Castillo, Josemar A.; Swing, Kelly; Hayes, Mark A.

doi:10.1016/j.ecolind.2014.08.036

Cited by 12 publications

(17 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequent intramolecular H transfer initiated by irradiation decompose the protein and HONO is emitted (adapted from Bejan et al, 2006). ticle mass in airborne particles (Franze et al, 2003a;Staton et al, 2015;Menetrez et al, 2007) and are also found at surfaces of soils and plants. Proteins can be nitrated and are then likely to enhance allergic responses (Gruijthuijsen et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Light-induced protein nitration and degradation with HONO emission

et al. 2017

View full text Add to dashboard Cite

Abstract. Proteins can be nitrated by air pollutants (NO 2 ), enhancing their allergenic potential. This work provides insight into protein nitration and subsequent decomposition in the presence of solar radiation. We also investigated lightinduced formation of nitrous acid (HONO) from protein surfaces that were nitrated either online with instantaneous gas-phase exposure to NO 2 or offline by an efficient nitration agent (tetranitromethane, TNM). Bovine serum albumin (BSA) and ovalbumin (OVA) were used as model substances for proteins. Nitration degrees of about 1 % were derived applying NO 2 concentrations of 100 ppb under VIS/UV illuminated conditions, while simultaneous decomposition of (nitrated) proteins was also found during long-term (20 h) irradiation exposure. Measurements of gas exchange on TNMnitrated proteins revealed that HONO can be formed and released even without contribution of instantaneous heterogeneous NO 2 conversion. NO 2 exposure was found to increase HONO emissions substantially. In particular, a strong dependence of HONO emissions on light intensity, relative humidity, NO 2 concentrations and the applied coating thickness was found. The 20 h long-term studies revealed sustained HONO formation, even when concentrations of the intact (nitrated) proteins were too low to be detected after the gas exchange measurements. A reaction mechanism for the NO 2 conversion based on the Langmuir-Hinshelwood kinetics is proposed.

show abstract

Section: Introductionmentioning

confidence: 99%

Light-induced protein nitration and degradation with HONO emission

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Nowadays, molecular genetic analysis techniques, such as quantitative polymerase chain reaction (qPCR) or next-generation sequencing (NGS), have gained ground, because they provide information not only on the viable and cultivable cells but also on uncultivable, dead or fragments of plant and animal cells . Alternative approach to determine particles of biological origin is based on chemical tracer techniques Gosselin et al, 2016;Hock et al, 2008;Schneider et al, 2011;Zhang et al, 2010;Staton et al, 2015;Rathnayake et al, 2017). The benefit of chemical tracers, such as carbohydrates, proteins/amino acids and lipids, is their applicability to quantitative analysis, while their main disadvantage is inability to identify different biological species.…”

mentioning

confidence: 99%

Characterization of free amino acids, bacteria and fungi in size-segregated atmospheric aerosols in boreal forest: seasonal patterns, abundances and size distributions

et al. 2017

View full text Add to dashboard Cite

Abstract. Primary biological aerosol particles (PBAPs) are ubiquitous in the atmosphere and constitute ∼ 30 % of atmospheric aerosol particle mass in sizes > 1 µm. PBAP components, such as bacteria, fungi and pollen, may affect the climate by acting as cloud-active particles, thus having an effect on cloud and precipitation formation processes. In this study, size-segregated aerosol samples (< 1.0, 1-2.5, 2.5-10 and > 10 µm) were collected in boreal forest (Hyytiälä, Finland) during a 9-month period covering all seasons and analysed for free amino acids (FAAs), DNA concentration and microorganism (bacteria, Pseudomonas and fungi). Measurements were performed using tandem mass spectrometry, spectrophotometry and qPCR, respectively. Meteorological parameters and statistical analysis were used to study their atmospheric implication for results. Distinct annual patterns of PBAP components were observed, late spring and autumn being seasons of dominant occurrence. Elevated abundances of FAAs and bacteria were observed during the local pollen season, whereas fungi were observed at the highest level during autumn. Meteorological parameters such as air and soil temperature, radiation and rainfall were observed to possess a close relationship with PBAP abundances on an annual scale.

show abstract

“…Can they be nitrated efficiently? To investigate the degree of protein nitration under illuminated conditions, BSA coated on the reaction tube (17.5 µg cm −2 ) was exposed to seven VIS lamps (40 % of a clear-sky irradiance for a solar zenith of 48 • ;Stemmler et al, 2006) and 100 ppb NO 2 at 70 % RH. After 20 h the BSA ND (concentration of nitrated tyrosine residues divided by the total concentration of tyrosine residues) investigated by means of the HPLC-DAD method was (1.0 ± 0.1) %, significantly higher than the ND of untreated BSA (0 %).…”

Section: Bsa Nitration and Degradationmentioning

confidence: 99%

“…It was suggested that HONO arises from the photolysis of nitric acid and nitrate or by heterogeneous photochemistry of NO 2 on organic substrates and soot (Zhou et al, 2001(Zhou et al, , 2002(Zhou et al, and 2003Villena et al, 2011;Ramazan et al, 2004;George et al, 2005;Sosedova et al, 2011;Monge et al, 2010;Han et al, 2016). Stemmler et al (2006Stemmler et al ( , 2007 found HONO formation on light-activated humic acid, and field studies showed that HONO formation correlates with aerosol surface area, NO 2 and solar radiation (Su et al, 2008;Reisinger, 2000;Costabile et al, 2010;Wong et al, 2012;Sörgel et al, 2015) and is increased during foggy periods (Notholt et al, 1992). Another proposed source of HONO is the soil, where it has been found to be co-emitted with NO by soil biological activities (Oswald et al, 2013;Su et al, 2011;Weber et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

response to referee 2

Meusel¹

2017

Preprint

View full text Add to dashboard Cite

Proteins can be nitrated by air pollutants (NO 2), enhancing their allergenic potential. This work provides insight into protein nitration and subsequent decomposition in the presence of solar radiation. We also investigated lightinduced formation of nitrous acid (HONO) from protein surfaces that were nitrated either online with instantaneous gas-phase exposure to NO 2 or offline by an efficient nitration agent (tetranitromethane, TNM). Bovine serum albumin (BSA) and ovalbumin (OVA) were used as model substances for proteins. Nitration degrees of about 1 % were derived applying NO 2 concentrations of 100 ppb under VIS/UV illuminated conditions, while simultaneous decomposition of (nitrated) proteins was also found during long-term (20 h) irradiation exposure. Measurements of gas exchange on TNMnitrated proteins revealed that HONO can be formed and released even without contribution of instantaneous heterogeneous NO 2 conversion. NO 2 exposure was found to increase HONO emissions substantially. In particular, a strong dependence of HONO emissions on light intensity, relative humidity, NO 2 concentrations and the applied coating thickness was found. The 20 h long-term studies revealed sustained HONO formation, even when concentrations of the intact (nitrated) proteins were too low to be detected after the gas exchange measurements. A reaction mechanism for the NO 2 conversion based on the Langmuir-Hinshelwood kinetics is proposed.

show abstract

Ground level environmental protein concentrations in various ecuadorian environments: Potential uses of aerosolized protein for ecological research

Cited by 12 publications

References 38 publications

Light-induced protein nitration and degradation with HONO emission

Light-induced protein nitration and degradation with HONO emission

Characterization of free amino acids, bacteria and fungi in size-segregated atmospheric aerosols in boreal forest: seasonal patterns, abundances and size distributions

response to referee 2

Contact Info

Product

Resources

About