Group II Nucleopolyhedrovirus Subgroups Revealed by Phylogenetic Analysis of Polyhedrin and DNA Polymerase Gene Sequences

Bulach, Dieter; Kumar, Chandan; Zaia, Angelo; Liang, Beibei; Tribe, D. E.

doi:10.1006/jipa.1998.4797

Cited by 121 publications

(95 citation statements)

References 51 publications

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“…Subsequently, Bulach et al 8 supported these clades and revealed other subclades within Group II by analyzing the combined Polh and DNA polymerase sequences. Our results were concordant with Zanotto et al 6 and Bulach et al 8 where HaNPV, SeNPV and SlNPV were allocated into Group II. However, BmNPVs revealed possibly paraphyletic relationships between the Canadian and the Asian isolates.…”

Section: Scienceasia Scienceasia Scienceasiamentioning

confidence: 99%

“…Based on phylogenetic studies using the amino acid sequences of polyhedron proteins, lepidopteran NPVs have been classified into two groups, namely Group I and Group II 6 . Subsequently, Bulach et al 8 supported these clades and revealed other subclades within Group II by analyzing the polyhedrin and DNA polymerase genes. Evolutionary studies of baculoviruses have been carried out with several additional genes, but systematics of these viruses is still consistent.…”

Section: Introductionmentioning

confidence: 99%

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Kaewwises¹,

Chaeychomsri²,

Chowpongpang³

et al. 2006

ScienceAsia

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ABSTRACT:The full-length of the polyhedrin gene (polh) of Thai Bombyx mori nucleopolyhedrovirus (BmNPV) was cloned and sequenced. The polh sequence contained a 735 bp open reading frame (ORF) encoding a protein of 245 amino acids with a predicted molecular mass of 28.8 kDa. The nucleotide sequence of Thai BmNPV polh shows greater than 98% identity to the sequences of five different BmNPV polh genes that were previously characterized. The high degree of sequence identity with the polh sequences of other BmNPVs suggests that they are orthologues of the BmNPV polh gene in this study. Comparison of Thai BmNPV polh sequence with other polhs of Lepidoteran NPVs (Autographa californica, Helicoverpa armigera, Spodoptera litura and S. exigua) indicated that the nucleotide and amino acid sequences were greater than 65% and 78% identical, respectively.

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Section: Scienceasia Scienceasia Scienceasiamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Kaewwises¹,

Chaeychomsri²,

Chowpongpang³

et al. 2006

ScienceAsia

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“…In contrast, the tree topology generated by the polyhedron (polh) gene placed AcMNPV in group II and it was suggested that the polh gene of AcMNPV might have recombined with that of a virus in group II [30][31][32] . The Lepidopteran NPVs were separated into two groups, group I and II, based on the conserved gene, polyhedron, which encoded the major occlusion body protein required for producing viral progeny 3,5 . Later, the phylogenetics of NPVs were generated with other conserved genes, www.scienceasia.org for example lef-2 33 , lef-8, lef-9 34,35 , and ecdysteroid UBP-glycosyl transferase (egt) 4 .…”

Section: Phylogenetic Analysis Of Group Imentioning

confidence: 99%

“…The Lepidopteran NPVs were first separated into group I and group II, based on the polyhedrine gene 3 . Such division into two groups has been supported by phylogenetic analysis based on other genes [4][5][6][7] .…”

Section: Introductionmentioning

confidence: 97%

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Chotimanothum¹,

Pinpart²,

Ngernsiri³

2011

ScienceAsia

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The immediate early 1 (ie-1) gene is essential for DNA replication of nucleopolyhedrosis virus. Here, the gene ie-1 from nucleopolyhedrovirus (Nanglai-BmNPV) infecting a local silkworm (Bombyx mori), Nanglai strain, was cloned and sequenced. The gene contained a 1755 bp open reading frame encoding a deduced protein of 584 amino acids, molecular weight 66.9 kDa, and isoelectric point 5.79. The nucleotide and amino acid sequences of Nanglai-BmNPV ie-1 gene showed, respectively, 98 and 97% identity to those of the BmNPV ie-1 genes. The identity of the Nanglai-BmNPV IE-1 deduced amino acid sequence with IE-1 protein sequences of other 37 known Lepidopteran NPVs varied from 20% up to 97%. The phylogenic tree established with the IE-1 protein sequences of the Lepidopteran NPVs showed similar topology to those previously reported using highly conserved genes. Interestingly, the tree obtained with IE-1 clearly shows that the Lepidopteran NPVs group II clade can be subdivided into three subgroups.

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“…The family Baculoviridae is subdivided into two genera, Nucleopolyhedrovirus (NPV) and Granulovirus (GV). Based on the polyhedrin (polh) gene, NPVs are further subdivided into group I and group II (Bulach et al, 1999;Herniou et al, 2001Herniou et al, , 2003. Recent evidence shown from the comparison of 29 baculovirus genomes indicates that baculovirus phylogeny is aligned with the classification of the hosts more closely than with morphological traits (Afonso et al, 2001;Garcia-Maruniak et al, 2004;Lauzon et al, 2004;Jehle et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus

Chen

Lee

et al. 2008

Journal of General Virology

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The complete genome of the Maruca vitrata nucleopolyhedrovirus (MaviNPV) isolated from the legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae), was sequenced. It was found to be 111 953 bp in length, with an overall 39 % G+C content, and contained 126 open reading frames (ORFs) encoding predicted proteins of over 50 aa. The gene content and gene order of MaviNPV have the highest similarity to those of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and their shared homologous genes are 100 % collinear. In fact, MaviNPV seems to be a mini-AcMNPV that is native to Taiwan and possesses a smaller genome with fewer auxiliary genes than the AcMNPV type species. Except for one ORF (Mv74), all of the MaviNPV ORFs have homologues in the AcMNPV genome. MaviNPV is the first lepidopteranspecific baculovirus to lack homologues of vfgf and odv-e66. In addition, MaviNPV lacks the baculovirus repeat ORF (bro) gene that corresponds to AcMNPV ORF2. Five homologous regions (hrs) were located within the MaviNPV genome, and these contained a total of 44 imperfect palindromes. Phylogenetic analysis of the whole genome revealed that MaviNPV was separated from the common ancestor of AcMNPV and Bombyx mori nucleopolyhedrovirus before these two viral species diverged from each other. Moreover, replication of MaviNPV in several cell lines and an egfp-MaviNPV infection assay revealed that IPLB-LD-652Y cells are only partially permissive to MaviNPV, which supports our conclusion that MaviNPV is a distinct species of the group I lepidopteran NPVs.

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Group II Nucleopolyhedrovirus Subgroups Revealed by Phylogenetic Analysis of Polyhedrin and DNA Polymerase Gene Sequences

Cited by 121 publications

References 51 publications

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Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus

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