Group joining behaviours in the entomopathogenic nematode Steinernema glaseri

Stevens, G. H.; Erdoğan, Hilal; Pimentel, Eleanor; Dotson, Jenna; Stevens, Asa; Shapiro‐Ilan, David I.; Kaplan, Fatma; Schliekelman, Paul; Lewis, Edwin E.

doi:10.1016/j.biocontrol.2023.105220

Cited by 9 publications

(1 citation statement)

References 20 publications

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“…Dead larvae were identified after 7 days and individually placed in modified white traps [ 55 ]. Nematodes harbouring bacterial symbionts were first identified by morphological traits and placed in the intergeneric “glaseri group” [ 56 ]. Morphological and morphometric studies were later supplemented with molecular methods, including ITS and 28S rDNA gene sequence analysis, as previously described [ 57 ].…”

Section: Methodsmentioning

confidence: 99%

Genome Sequence Analysis of Native Xenorhabdus Strains Isolated from Entomopathogenic Nematodes in Argentina

Palma,

Frizzo,

Kaiser

et al. 2024

Toxins

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Entomopathogenic nematodes from the genus Steinernema (Nematoda: Steinernematidae) are capable of causing the rapid killing of insect hosts, facilitated by their association with symbiotic Gram-negative bacteria in the genus Xenorhabdus (Enterobacterales: Morganellaceae), positioning them as interesting candidate tools for the control of insect pests. In spite of this, only a limited number of species from this bacterial genus have been identified from their nematode hosts and their insecticidal properties documented. This study aimed to perform the genome sequence analysis of fourteen Xenorhabdus strains that were isolated from Steinernema nematodes in Argentina. All of the strains were found to be able of killing 7th instar larvae of Galleria mellonella (L.) (Lepidoptera: Pyralidae). Their sequenced genomes harbour 110 putative insecticidal proteins including Tc, Txp, Mcf, Pra/Prb and App homologs, plus other virulence factors such as putative nematocidal proteins, chitinases and secondary metabolite gene clusters for the synthesis of different bioactive compounds. Maximum-likelihood phylogenetic analysis plus average nucleotide identity calculations strongly suggested that three strains should be considered novel species. The species name for strains PSL and Reich (same species according to % ANI) is proposed as Xenorhabdus littoralis sp. nov., whereas strain 12 is proposed as Xenorhabdus santafensis sp. nov. In this work, we present a dual insight into the biocidal potential and diversity of the Xenorhabdus genus, demonstrated by different numbers of putative insecticidal genes and biosynthetic gene clusters, along with a fresh exploration of the species within this genus.

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Section: Methodsmentioning

confidence: 99%