Growth and Controlled Morphogenesis in Pea Root Callus Tissue Grown in Liquid Media

Suspension cultures of higher plant cells in synthetic media

“…Tn each case, callus tissues which ha(l been previously well established in agar tube culture on complex medlia were used for inocula. A clone of carrot tissue (Dancuts carota L.) growinig on White's medium + 5 % coconut milk + 5 X 10-8 g/ml 2,4-D previously studiedl by Reinert (12) was used; a clone of root callus tissue isolated from excised root cultures of Convolv'tlus arvensis L. was maintained in an agar medium (M6) devised earlier (20) for pea root callus containing 1 g/I yeast extract and 10-6) %r 2,4-D. Also tested was a clone of callus tissue derived from the hypocotyl of seedlings of Haplopapputs gracilis (Nutt.) Gray maintainied on White's mledium +5 % coconut milk + 5 x 10-8 g/ml 2,4-D (13).…”

“…creases in fresh weight of the cell population and the number of cells in suspension were of the same order of magnitude in the synthetic medium as on the complex media.EFFECT OF AUXIN & CERTAIN GROWTH FACTORS ON T1SSUE WEIGHT & DISSOCIATION.In experiments with pea root callus tissue(20) it had been observed that the degree of tissue dissociation depended upon the relative concentration of 2,4-D and of the yeast extract added to the medium. Experiments were set up to see if auxin played a similar important role in the synthetic medium tested here and if any of the other growth factors present in the synthetic medium affected the separation of cells.…”

Tissue Culture of Legumes for Crop Improvement

“…More recently, it has also been observed that 7% of the leaflets (5 -6 mm long) dissected from 20-day-old greenhouse-grown plants of 'Century' were able to regenerate plants. Despite the progress made in inducing plant regeneration from primary callus, similar attempts with long-term callus remain unsuccessful (Rubluo et al, unpublished; also see Torrey and Shigemura 1957;Torrey 1967).…”

Natural and induced variation in tissue culture

“…Variation in plant tissue culture also includes changes in the growth habit, rates, appearance, and requirements. Callus cultures of Picea glauca (REINERT, 1956) pea (TORREY & SHIGEMURA, 1957), tobacco (SIEVERT & HILDEBRANDT, 1965) carrot (BLAKELY & STEWARD, 1964;MUIR, 1965) wheat (NAKAI & SHIMADA, 1975), Trichocereus spp. (STEINHART, 1962) and Haplopuppus gracilis (AMANO, 1972) have exhibited differences in growth habit with some clones exhibiting compact growth and others having very friable callus.…”