Growth and Functionality of Cells Cultured on Conducting and Semi-Conducting Surfaces Modified with Self-Assembled Monolayers (SAMs)

Aithal, Rajendra K.; Doss, Amber T.; Kumaraswamy, Deepak P.; Mills, David K.; Kuila, Debasish

doi:10.3390/coatings6010009

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…Silanes are generally known to form self-assembled monolayers when deposited onto a glass surface and thus offer a simple way to change the surface properties through display of specific functional groups . It was found that APTES preserves the viability and proliferation of human dermal fibroblast and does not deteriorate the potency of mesenchymal stem cells . To validate this surface treatment, we flow nonviable cells through a device with a ridge angle of 15° with and without the triethoxysilane treatment.…”

Section: Resultsmentioning

confidence: 99%

Microfluidic Platform to Transduce Cell Viability to Distinct Flow Pathways for High-Accuracy Sensing

et al. 2021

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Mechanical properties of cells such as stiffness can act as biomarkers to sort or detect cell functional properties such as viability. In this study, we report the use of a microfluidic device as a high-sensitivity sensor that transduces cell biomechanics to cell separation to accurately detect viability. Cell populations are flowed and deflected at a number of skew ridges such that deflection per ridge, cell−ridge interaction time, and cell size can all be used as sensor inputs to accurately determine the cell state. The angle of the ridges was evaluated to optimize the differences in cell translation between viable and nonviable cells while allowing continuous flow. In the first mode of operation, we flowed viable and nonviable cells through the device and conducted a sensitivity analysis by recording the cell's total deflection as a binary classifier that differentiates viable from nonviable cells. The performance of the sensor was assessed using an area under the curve (AUC) analysis to be 0.97. By including additional sensor inputs in the second mode of operation, we conducted a principal component analysis (PCA) to further improve the identification of the cell state by clustering populations with little overlap between viable and nonviable cells. We therefore found that microfluidic separation devices can be used to efficiently sort cells and accurately sense viability in a label-free manner.

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Section: Resultsmentioning

confidence: 99%

Microfluidic Platform to Transduce Cell Viability to Distinct Flow Pathways for High-Accuracy Sensing

et al. 2021

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“…Recently, several novel applications of ITO have emerged. Aithal et al [31] observed maintenance of cell phenotype and long-term functionality of human dermal fibroblast (HDF) cells on ITO modified by self-assembled monolayers (SAMs). This important advance is significant for the future development of bioengineered skins.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Optically–Excited THz Wave Emission by GaAs Coated with a Rough ITO Thin Film

Sahoo

Kang

et al. 2023

Coatings

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In this study, we report enhancement of terahertz (THz) radiation with indium-tin-oxide (ITO) thin-film deposited on semi-insulating gallium arsenide substrate (SI-GaAs). The amplitude of THz emission from both ITO/SI-GaAs and bare SI-GaAs substrate as a function of optical pump (i) incident angle, (ii) polarization angle, and (iii) power were investigated. The enhancement of peak amplitude of a THz pulse transmitted through the ITO/SI-GaAs sample in comparison to bare SI-GaAs substrate varied from 100% to 0% when the pump incidence angle changed from 0° to 50°. The maximum enhancement ratio of peak amplitude for a coated sample relative to the bare substrate is approximately up to 2.5 times at the minimum pump intensity of 3.6 TW/m2 and gradually decreased to one at the maximum pump intensity of 20 TW/m2. From outcomes of these studies, together with data on surface and material characterization of the samples, we show that THz emission originates from the ITO/GaAs interfaces. Further, both interface-field-induced transient current and field-induced optical rectification contribute to the observed THz signal. Observed enhancement was tentatively attributed to surface-plasmon-induced local field enhancement, coupled with constructive interference of forward and retro-reflected backward THz emission from the ITO/GaAs interfaces. The polarity-flip reported previously for very thin Au-coated GaAs was not observed. This was explained by the wide-bandgap, transparency and lower free carriers of ITO. For best results, the incident angle should be in the range of 0 to 30° and the incident polarization should be 0 to 45°. We further predict that the ITO thin film of suitable thickness or with engineered nanostructures, post-annealed under optimum conditions may lead to further enhancement of THz radiation from ITO-coated semiconductor surfaces.

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Growth and Functionality of Cells Cultured on Conducting and Semi-Conducting Surfaces Modified with Self-Assembled Monolayers (SAMs)

Cited by 2 publications

References 36 publications

Microfluidic Platform to Transduce Cell Viability to Distinct Flow Pathways for High-Accuracy Sensing

Microfluidic Platform to Transduce Cell Viability to Distinct Flow Pathways for High-Accuracy Sensing

Enhanced Optically–Excited THz Wave Emission by GaAs Coated with a Rough ITO Thin Film

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