Growth Behavior and Transcriptome Profile Analysis of <i>Proteus mirabilis</i> Strain Under Long- versus Short-Term Simulated Microgravity Environment

Bin, Zhang; Bai, Po; Wang, Dapeng

doi:10.33073/pjm-2022-015

Cited by 2 publications

(2 citation statements)

References 47 publications

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“…They observed reduced biofilm formation after 64 days and following transcriptional analysis proposed that this reduction was potentially due to downregulation of the pil and algR genes associated with conjugative pili and alginate biosynthesis, or upregulation of genes involved in metal iron binding (as available iron increases biofilm formation) 443 . In another study analyzing Proteus mirabilis biofilm formation following short-term or long-term SMG, researchers found that long-term exposure resulted in downregulation of genes associated with fimbriae, impeding adhesion, and ultimately decreasing biofilm formation 444 .…”

Section: Negative Impact Of Microbes and Mitigation Strategiesmentioning

confidence: 99%

Microbial applications for sustainable space exploration beyond low Earth orbit

Koehle,

Brumwell,

Seto

et al. 2023

npj Microgravity

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With the construction of the International Space Station, humans have been continuously living and working in space for 22 years. Microbial studies in space and other extreme environments on Earth have shown the ability for bacteria and fungi to adapt and change compared to “normal” conditions. Some of these changes, like biofilm formation, can impact astronaut health and spacecraft integrity in a negative way, while others, such as a propensity for plastic degradation, can promote self-sufficiency and sustainability in space. With the next era of space exploration upon us, which will see crewed missions to the Moon and Mars in the next 10 years, incorporating microbiology research into planning, decision-making, and mission design will be paramount to ensuring success of these long-duration missions. These can include astronaut microbiome studies to protect against infections, immune system dysfunction and bone deterioration, or biological in situ resource utilization (bISRU) studies that incorporate microbes to act as radiation shields, create electricity and establish robust plant habitats for fresh food and recycling of waste. In this review, information will be presented on the beneficial use of microbes in bioregenerative life support systems, their applicability to bISRU, and their capability to be genetically engineered for biotechnological space applications. In addition, we discuss the negative effect microbes and microbial communities may have on long-duration space travel and provide mitigation strategies to reduce their impact. Utilizing the benefits of microbes, while understanding their limitations, will help us explore deeper into space and develop sustainable human habitats on the Moon, Mars and beyond.

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Section: Negative Impact Of Microbes and Mitigation Strategiesmentioning

confidence: 99%

Microbial applications for sustainable space exploration beyond low Earth orbit

Koehle,

Brumwell,

Seto

et al. 2023

npj Microgravity

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show abstract

“…GO enrichment analysis of differentially expressed genes was implemented by the top GO R package, in which gene length bias was corrected [27] . GO terms with corrected p<0.05 were considered significantly enriched by differential expressed genes.…”

Section: Gene Ontology (Go) and Kyoto Encyclopedia Of Genes And Genom...mentioning

confidence: 99%

Model Construction and Muscle Transcriptome Study of Zebrafish with Laminin-Alpha 2 Muscular Dystrophy

Zhou,

Huang,

Wang

2023

IJPS

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The purpose of this study was to simulate muscular dystrophy disease by constructing a laminin-alpha 2 zebrafish model, and to study the changes of muscle transcriptome using transcriptomic methods. Using clustered regularly interspaced short palindromic repeats associated protein 9 gene editing technology; the zebrafish laminin-alpha 2 gene was knocked out or mutated. First, the appropriate guide RNA sequence was designed, and then the guide RNA and clustered regularly interspaced short palindromic repeats associated protein 9 proteins were synthesized and injected into zebrafish embryos. The lamininalpha 2 model fish is screened and identified by detecting the mutation of DNA or messenger RNA in the progeny fish genome. The muscle phenotype identification results of zebrafish model 4 showed that the muscle phenotypes of normal and unable swimming individuals in laminin-alpha 2 knockout group were abnormal, while the muscle phenotypes of clustered regularly interspaced short palindromic repeats associated protein 9 control group were not. The muscular dystrophy characteristics of laminin-alpha 2 model fish were confirmed. Functional enrichment and pathway analysis of differentially expressed genes were performed to further reveal the biological processes and regulatory pathways associated with muscular dystrophy in laminin-alpha 2 model fish. Transcriptome sequencing analysis revealed the difference in transcriptome expression between the muscle tissue of laminin-alpha 2 zebrafish model and wild-type zebrafish. These differentially expressed genes are involved in biological processes such as muscle development, metabolism and signal transduction, providing important clues for further understanding of the pathogenesis of muscular dystrophy. Through functional enrichment and pathway analysis, the key signaling pathways and molecular mechanisms involved in the pathogenesis of muscular dystrophy were identified. These findings provide theoretical basis for the discovery of new therapeutic targets and formulation of corresponding therapeutic strategies, and provide new ideas and methods for the treatment of muscle diseases.

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Growth Behavior and Transcriptome Profile Analysis of Proteus mirabilis Strain Under Long- versus Short-Term Simulated Microgravity Environment

Cited by 2 publications

References 47 publications

Microbial applications for sustainable space exploration beyond low Earth orbit

Microbial applications for sustainable space exploration beyond low Earth orbit

Model Construction and Muscle Transcriptome Study of Zebrafish with Laminin-Alpha 2 Muscular Dystrophy

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