The weight and thickness of the two ventricular walls of the heart were measured in 80 newborn puppies. These puppies were born in the dog colony, the descendants of a typical heterogeneous collection of dogs, for student use in the laboratory.The hearts of these dogs average 2.07 gm in weight. The left ventricular wall is slightly heavier and also slightly more variable in weight than the right wall. The two free ventricular walls comprise 56% of the weight of the entire heart. The ratio of the weight of the left to the right ventricular wall, at birth, averages 1.0078.The left wall is also slightly thicker than the right wall. The thickness of the right wall is more variable than the left. The ratio of the thickness of the left to the right ventricular wall is 1.036.These data are compared with other available measurements on adult dog hearts and on human hearts.