Growth characteristics and lipid content of three Korean isolates of Botryococcus braunii (Trebouxiophyceae)

Abstract

Three strains of the green microalga Botryococcus braunii (JJS, KCM, and KJD) were isolated from different water bodies in Korea and grown as batch cultures in the laboratory. The effects of different growth media and temperatures on the growth rate were investigated, as well as the effect of temperature on the total lipid content and lipid profile. All three strains had the highest growth rates in BG-11 medium and at 25°C. Maximal lipid production (g L -1) was at 30°C in the JJS strain and at 25°C in the KCM and KJD strains. However, all the three strains produced the greatest percent dry weight of total lipids at 15°C and had the lowest percent dry weight of total lipids at 25°C. In general, oleic acid, linolenic acid, and behenic acid were the most common fatty acids in all three strains. However, the three strains varied considerably in their fatty acid profiles at different culture temperatures.

Оцінювання Продуктивності Культивування Мікроводоростей Для Виробництва Біопалива В Умовах України

“…Energy amount, stored in the biomass unit of cultivated microalgae was taken biom Е = 30.0 MJ/kg [20,21]. Daily amount of lipids, accumulated in the biomass of microalgae by the formula (8), the coefficient of lipid content in microalgaes biomass was taken lip k = 0,2, which, according to [21,[23][24][25], is one of the lowes possible. Density of lipids, accumulated in the biomass, was taken lip ρ = 0.85 kg/liter [26].…”

Reduction of Microalgae by Cupper Ion In Impressed Current Anti Fouling System for Biofouling Prevention in Saline Environment

“…The temperature during the testing was 27-28 o C. Lupi et al (1991) reported that Botryococcus braunii cannot grow at temperature above 32 o C, and there were no previous studies which investigated the effect of temperatures below 18 o C for the growth of Botryococcus braunii. According to Lee et al, (2015) Botryococcus microorganisms showed a variety of pH adaptability (Dayananda et al, 2017). The population of Botryococcus sp in the operation of a simple ICAF system was shown in Figure 12.…”

Extracellular Metabolites from Industrial Microalgae and Their Biotechnological Potential

“…Among oleaginous microalgae, the colonial green alga B. braunii accumulates large quantities of hydrocarbons mainly in the extracellular space [109,110]. Cells of B. braunii associate via a unique colonial organization mediated by an extracellular matrix (ECM) mainly composed of long-chain polyacetal hydrocarbons which are cross-linked with hydrocarbons.…”

“…Among several extraction solvents, heptane did not damage B. braunii if it was in contact for less than 20 min. In addition, a solvent-free method of gentle pressure (i.e., “blotting”) was introduced as alternative for external oil recovery by Moheimani et al [109]. For the study of the long-term reliability of this oil recovery process, an integrated reactor was designed.…”