Growth inhibition and recovery patterns of common duckweed Lemna minor L. after repeated exposure to isoproturon

Abstract

Aquatic non-targeted organisms are more likely to be exposed to herbicides in multiple pulse events then long continuous exposure. The potential of an organism to recover between exposures has an important role in the overall effects of the toxicant. Common duckweeds show high potential for recovery after a single exposure to isoproturon. To evaluate the growth patterns and recovery potential between multiple exposures, L. minor plants were exposed to isoproturon in three repetitive 7-day treatment cycles in three time-variable exposure scenarios with equivalent time-weighted average concentrations. The growth was significantly inhibited during each exposure phase with significant cumulative effects in every subsequent treatment cycle resulting in a cumulative decrease in biomass production. However, inhibitory effects were reversible upon transferring plants to a herbicide-free nutrient solution. These results indicate that L. minor plants have a high recovery potential even after multiple exposures to isoproturon. Observed cumulative decrease in biomass production, as well as the potential for fast and efficient recovery from repeated herbicide exposure, might affect the competitiveness of L. minor in surface water communities. The observations made during each exposure period, recovery patterns, and the resulting cumulative effects over time may contribute to further development, calibration and validation of mechanistic toxicokinetic/toxicodynamic models for simulating the effects of pesticides on aquatic plants populations in the laboratory and environmental conditions.

Duckweeds for Phytoremediation of Polluted Water

“…Duckweed was severely inhibited by norflurazon, but there was a rapid recovery for all norflurazon concentrations tested after the plant was removed from the media [ 79 ]. Varga et al (2020) evaluated the growth patterns and recovery potential of duckweed between multiple exposures to the herbicide isoproturon [ 80 ]. Growth was significantly inhibited during each exposure phase with significant cumulative effects in subsequent treatment cycles, resulting in a cumulative decrease in biomass production.…”