Growth Kinetics of Gold Nanoparticle Formation from Glycated Hemoglobin

Madhavan, Ashwathi Asha; Juneja, Subhavna; Moulick, Ranjita Ghosh; Bhattacharya, Jaydeep

doi:10.1021/acsomega.9b02200

Cited by 9 publications

(4 citation statements)

References 41 publications

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“…Finally, the corresponding data planes ( hkl ) were shown (Table S1). In conclusion, these findings support a crystalline phase of the synthesized GNP being composed of an inverted spinal structure. , FESEM, HR-TEM, and the EDX map confirmed the purity of elemental gold NPs (Figure a, e, i–k) …”

Section: Resultssupporting

confidence: 76%

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Inhibition Of Tau Protein Aggregation By a Chaperone-like β-Boswellic Acid Conjugated To Gold Nanoparticles

Gharb

Nouralishahi

Riazi³

et al. 2022

ACS Omega

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A potential therapeutic strategy to inhibit tau protein aggregation in neurons has substantial effects on preventing or controlling Alzheimer’s disease (AD). In this work, we designed a covalent and noncovalent conjugation of β-boswellic acid (BA) to gold nanoparticles (GNPs). We provided the opportunity to investigate the effect of the surface composition of BA-GNPs on the aggregation of the tau protein 1N/4R isoform in vitro. HR-TEM and FESEM micrographs revealed that GNPs were spherical and uniform, smaller than 25 nm. According to UV–visible and FTIR data, BA was successfully conjugated to GNPs. The finding illustrates the effect of the surface charge, size, and hydrophobicity of BA-GNPs on the kinetics of tau protein aggregation. The size and surface area of U-G-BA demonstrated that inhibited tau aggregation more effectively than covalently linked BA. The proposed method for preventing tau aggregation was monomer reduction. At the same time, a chaperone-like feature of GNP-BA while sustaining a tau native structure prevented the additional formation of fibrils. Overall, this study provides insight into the interaction of GNP-BAs with a monomer of tau protein and may suggest novel future therapies for AD.

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Section: Resultssupporting

confidence: 76%

“…In conclusion, these findings support a crystalline phase of the synthesized GNP being composed of an inverted spinal structure. 45,46 FESEM, HR-TEM, and the EDX map confirmed the purity of elemental gold NPs (Figure 2a, e, i−k). 47 Characterization of Drug Loading Efficiency.…”

Section: ■ Results and Discussionmentioning

confidence: 59%

Inhibition Of Tau Protein Aggregation By a Chaperone-like β-Boswellic Acid Conjugated To Gold Nanoparticles

Gharb

Nouralishahi

Riazi³

et al. 2022

ACS Omega

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“…The above discussion was focused on the extraction of protein conjugated Au NPs made from water-insoluble proteins. In this section, protein coated Au NPs are prepared in the presence of well-known water-soluble model proteins such as BSA, lysozyme, and hemoglobin under similar reaction conditions (Figure S8), and the results are compared in Table . Unlike the predominantly hydrophobic proteins mentioned above, these proteins are readily soluble in pure water and hence are directly used in the in situ synthesis of Au NPs.…”

Section: Resultsmentioning

confidence: 99%

Extraction of Bionanomaterials from the Aqueous Bulk by Using Surface Active and Water-Soluble Magnetic Nanoparticles

Kaur¹,

Khullar²,

Gupta

et al. 2021

Langmuir

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Surface active and water-soluble magnetic nanoparticles (NPs) were used to demonstrate the extraction of bionanomaterials from the aqueous bulk. Au NPs conjugated with different water-insoluble and water-soluble proteins were used as model bionanomaterials. UV–visible studies, zeta potential, and microscopic analyses were performed to quantify the extraction. Sodium dodecyl sulfate and dimethylene bis(dodecyldimethylammonium bromide) (12-2-12) stabilized surface active magnetic NPs were fully capable of extracting Au NPs conjugated with predominantly hydrophobic proteins from the aqueous bulk when placed at the aqueous–air interface. However, they were poor in extracting Au NPs from the aqueous bulk which were coated with predominantly hydrophilic water-soluble protein. On the other hand, water-soluble dodecyldimethyl-3-ammonio-1-propanesulfonate stabilized magnetic NPs proved to be fully capable of extracting all kinds of Au NPs conjugated with either water-soluble or water-insoluble proteins. The results highlight the remarkable ability of magnetic NPs in the extraction of bionanomaterials when placed at either biointerfaces or in the aqueous bulk of biological systems.

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“…As expected, as the reaction proceeds all the curves show an increase in the intensity of the SPR peak, which accounts for an enhancement of the number of particles produced along the time line. In addition, the absorption maxima shifted in all cases to the left (centering around of 520 nm), denoting an increase in the particle number accompanied by a reduction in particle size in time [70].…”

Section: Other Bioapplications: Screening Of Synthesis Parametersmentioning

confidence: 89%

Novel Microsynthesis of High-Yield Gold Nanoparticles to Accelerate Research in Biosensing and Other Bioapplications

Díaz-García,

Haensgen,

Inostroza

et al. 2023

Biosensors

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Gold nanoparticles (AuNPs) exhibit unique properties that make them appealing for applications in biosensing and other emerging fields. Despite the availability of numerous synthesis methods, important questions remain to be addressed regarding the volume effect on the synthesis yield and quality of AuNPs in the light of biosensing research. The present study addresses these issues by developing a novel microvolumetric citrate-reduction method to improve the synthesis of AuNPs, which were characterized by electronic microscopy, energy dispersive spectroscopy, zeta potential and colorimetric analysis. A comparison of the novel microsynthesis method with the standard Turkevich method demonstrated its superior performance in terms of yield, monodispersity, rapidity (in one step), reproducibility, and stability. The analytical behavior of AuNPs-based aptasensors prepared by microsynthesis was investigated using kanamycin detection and showed higher reproducibility and improved detection limits (3.4 times) compared to those of Turkevich AuNPs. Finally, the effect of pH was studied to demonstrate the suitability of the method for the screening of AuNP synthesis parameters that are of direct interest in biosensing research; the results showed an optimal pH range between 5.0 and 5.5. In summary, the approach described herein has the potential to improve research capabilities in biosensing, with the added benefits of lowering costs and minimizing waste generation in line with current trends in green nanotechnology.

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Growth Kinetics of Gold Nanoparticle Formation from Glycated Hemoglobin

Cited by 9 publications

References 41 publications

Inhibition Of Tau Protein Aggregation By a Chaperone-like β-Boswellic Acid Conjugated To Gold Nanoparticles

Inhibition Of Tau Protein Aggregation By a Chaperone-like β-Boswellic Acid Conjugated To Gold Nanoparticles

Extraction of Bionanomaterials from the Aqueous Bulk by Using Surface Active and Water-Soluble Magnetic Nanoparticles

Novel Microsynthesis of High-Yield Gold Nanoparticles to Accelerate Research in Biosensing and Other Bioapplications

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