Growth models from a brassinosteroid perspective

Ackerman-Lavert, M.; Savaldi-Goldstein, Sigal

doi:10.1016/j.pbi.2019.10.008

Cited by 41 publications

(37 citation statements)

References 81 publications

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“…Below: (where applicable) heatmaps of indirect (downstream) FT-dependent gene expression changes of known pathway activators (red in sidebar) or repressors (blue in sidebar). Indirect target genes include: abscisic acid: upregulation of some positive regulators of abscisic acid response ( ABF2 ) and repression of others ( PYL1 , PYR1 , XERICO ) 58 , 59 ; Brassinosteroid: upregulation of multiple positive signalling pathway components ( BRL2 , BRL3 , BSK1, BSK8 , BSL1 and BIN4 ) 123 . Cytokinin: upregulation of cytokinin production ( IPT3 ) and perception ( WOL ) and downregulation of negative cytokinin response regulators ( ARR7, ARR15 ) 124 .…”

Section: Resultsmentioning

confidence: 99%

“…This upstream regulatory region drives reporter expression in similar domains as endogenous LFY 66 . The requirement of the bZIP motifs for LFY upregulation in the context of the genomic construct, which contains the 2.3 kb 'LFY ; Brassinosteroid: upregulation of multiple positive signalling pathway components (BRL2, BRL3, BSK1, BSK8, BSL1 and BIN4) 123 . Cytokinin: upregulation of cytokinin production (IPT3) and perception (WOL) and downregulation of negative cytokinin response regulators (ARR7, ARR15) 124 .…”

Section: Discussionmentioning

confidence: 99%

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TERMINAL FLOWER 1-FD complex target genes and competition with FLOWERING LOCUS T

et al. 2020

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Plants monitor seasonal cues to optimize reproductive success by tuning onset of reproduction and inflorescence architecture. TERMINAL FLOWER 1 (TFL1) and FLOWERING LOCUS T (FT) and their orthologs antagonistically regulate these life history traits, yet their mechanism of action, antagonism and targets remain poorly understood. Here, we show that TFL1 is recruited to thousands of loci by the bZIP transcription factor FD. We identify the master regulator of floral fate, LEAFY (LFY) as a target under dual opposite regulation by TFL1 and FT and uncover a pivotal role of FT in promoting flower fate via LFY upregulation. We provide evidence that the antagonism between FT and TFL1 relies on competition for chromatin-bound FD at shared target loci. Direct TFL1-FD regulated target genes identify this complex as a hub for repressing both master regulators of reproductive development and endogenous signalling pathways. Our data provide mechanistic insight into how TFL1-FD sculpt inflorescence architecture, a trait important for reproductive success, plant architecture and yield.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

TERMINAL FLOWER 1-FD complex target genes and competition with FLOWERING LOCUS T

et al. 2020

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“…BRs regulate a variety of developmental and physiological processes in plants including cell division, photomorphogenesis, reproductive organ development, stomata development, leaf senescence, and biotic and abiotic stress responses ( Wang et al., 2012 ; Gruszka, 2013 ; Zhiponova et al., 2013 ; Wang et al., 2001 ; Planas-Riverola et al., 2019 ; Yu et al, 2018 ; Ackerman-Lavert and Savaldi-Goldstein, 2020 ). Consistently, the molecular crosstalk between the BR signaling pathway and other hormonal and developmental signaling pathways is starting to emerge.…”

Section: Introductionmentioning

confidence: 99%

Brassinosteroids Regulate Circadian Oscillation via the BES1/TPL-CCA1/LHY Module in Arabidopsis thaliana

et al. 2020

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Summary Brassinosteroids (BRs) regulate a variety of physiological processes in plants via extensive crosstalk with diverse biological signaling networks. Although BRs are known to reciprocally regulate circadian oscillation, the molecular mechanism underlying BR-mediated regulation of circadian clock remains unknown. Here, we demonstrate that the BR-activated transcription factor bri1 -EMS-SUPPRESSOR 1 (BES1) integrates BR signaling into the circadian network in Arabidopsis. BES1 repressed expression of CIRCADIAN CLOCK-ASSOCIATED 1 ( CCA1 ) and LATE ELONGATED HYPOCOTYL ( LHY ) at night by binding to their promoters, together with TOPLESS (TPL). The repression of CCA1 and LHY by BR treatment, which occurred during the night, was compromised in bes1-ko and tpl-8 mutants. Consistently, long-term treatment with BR shortened the circadian period, and BR-induced rhythmic shortening was impaired in bes1-ko and tpl-8 single mutants and in the cca1-1lhy-21 double mutant. Overall, BR signaling is conveyed to the circadian oscillator via the BES1/TPL-CCA1/LHY module, contributing to gating diurnal BR responses in plants.

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“…Auxin: upregulation a positive auxin response regulator ( ARF6 ) and downregulation of negative auxin response regulators ( IAAs ) 55 . Brassinosteroid: upregulation of multiple positive signalling pathway components ( BRL2 , BRL3 , BSK1, BSK8 , BSL1 and BIN4 ) 56 . Cytokinin: upregulation of cytokinin production ( IPT3 ) and perception ( WOL ) and downregulation of negative cytokinin response regulators ( ARR7, ARR15 ) 57 .…”

Section: Mainmentioning

confidence: 99%

Florigen family chromatin recruitment, competition and target genes

Zhu

Klasfeld

et al. 2020

Preprint

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17Plants monitor seasonal cues, such as day-length, to optimize life history traits including 18 onset of reproduction and inflorescence architecture 1-3 . Florigen family transcriptional 19 co-regulators TERMINAL FLOWER 1 (TFL1) and FLOWERING LOCUS T (FT) 20 antagonistically regulate these vital processes 4-6 yet how TFL1 and FT execute their 21 roles and what the mechanism is for their antagonism remains poorly understood. We 22 Main 35Plant development occurs after embryogenesis and is plastic, this allows modulation of 36 the final body plan in response to environmental cues to enhance growth and 37 reproductive success 7,8 . Of particular importance for species survival is the timing of the 38 formation of flowers that give rise to seeds which occurs in response to the seasonal 39 cues photoperiod and temperature 1,3,9 . For example, in plants that flower only once, like 40Arabidopsis and most crops, an early switch to flower formation allows rapid completion 41 of the life-cycle in a short growing season, but reduces total seed set or yield 10-12 . By 42 contrast, delaying flower formation supports formation of more seeds, but extends the 43 time to seed set. In many plant species, these alternative developmental trajectories are 44 tuned in response to daylength in antagonistic fashion by two members of the florigen 45 family of proteins 12,13 . FT promotes onset of the reproductive phase and flower 46 formation (determinacy), while TFL1 promotes vegetative development and branch fate 47 (indeterminacy) 4-6,13 . In Arabidopsis, which flowers in the spring, FT accumulates only 48 when the daylength exceeds a critical threshold, while TFL1 is present in both short-day 49 and long-day conditions 1,3,14 . A key unanswered question is how FT and TFL1 50 modulate plant form -what are the downstream processes they set in motion and what 51 is molecular basis for their antagonism? 52 53Accumulating evidence points to roles of FT and TFL1, which lack ability to bind DNA, 54 in transcriptional activation and repression, respectively, by forming complexes with a 55 bZIP transcription factor, FLOWERING LOCUS D (FD) 12,15-19 , although non-nuclear 56 functions for both proteins have also been described 20,21 . Mechanistic insight into 57 florigen activity has been hampered by their low protein abundance. To overcome this 58 limitation and to test the role of TFL1 in the nucleus, we conducted TFL1 chromatin 59 immunoprecipitation followed by sequencing (ChIP-seq). Towards this end, we first 60 generated a biologically active, genomic GFP-tagged version of TFL1 (gTFL1-GFP tfl1-61 1) (Supplementary Fig. 1a, b). Next, we enriched for TFL1 expressing cells by isolating 62 shoot apices from 42-day-old short-day-grown inflorescences just prior to onset of 63 flower formation (Fig. 1a). Finally, we optimized low abundance ChIP by combining 64 eight individual ChIP reactions per ChIP-seq replicate. We conducted FD ChIP-seq in 65 4 analogous fashion using a published 22 , biologically active ( Supplementary Fig. 1c), 66 genomic...

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Growth models from a brassinosteroid perspective

Cited by 41 publications

References 81 publications

TERMINAL FLOWER 1-FD complex target genes and competition with FLOWERING LOCUS T

TERMINAL FLOWER 1-FD complex target genes and competition with FLOWERING LOCUS T

Brassinosteroids Regulate Circadian Oscillation via the BES1/TPL-CCA1/LHY Module in Arabidopsis thaliana

Florigen family chromatin recruitment, competition and target genes

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