Growth performance, immune response, and digestive enzyme activity in Pacific white shrimp, Penaeus vannamei Boone, 1931, fed dietary microbial lysozyme

Javahery, Susan; Noori, Ahmad; Hoseinifar, Seyed Hossein

doi:10.1016/j.fsi.2019.06.049

Cited by 48 publications

(19 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[30,31], L. lactis [17], Lactobacillus plantarum [32], and P. acidilactici [33] demonstrated the response similar to our results. Furthermore, our results are similar to the observations of Javahery et al [34], who demonstrated that the administration of probiotics leads to enhanced digestion and nutrient absorption in the shrimp intestine, which was stimulated by endogenous enzymes produced by probiotics. Also, probiotic supplementation can improve the digestion of protein, starch, and fat in whiteleg shrimp compared to the CON diet, as a result of the increased value of enzyme activity [15].…”

Section: Discussionsupporting

confidence: 92%

Evaluation of Potential Probiotics Bacillus subtilis WB60, Pediococcus pentosaceus, and Lactococcus lactis on Growth Performance, Immune Response, Gut Histology and Immune-Related Genes in Whiteleg Shrimp, Litopenaeus vannamei

et al. 2020

View full text Add to dashboard Cite

An eight-week feeding trial was conducted to evaluate the effects of different dietary probiotic supplements in juvenile whiteleg shrimp, Litopenaeus vannamei. A basal control diet without probiotics (CON), and five other diets by supplementing Bacillus subtilis at 107 CFU/g diet (BS7), B. subtilis (BS8), Pediococcus pentosaceus (PP8), and Lactococcus lactis (LL8) at 108 CFU/g diet, and oxytetracycline (OTC) at 4 g/kg diet were used. Whiteleg shrimp with initial body weights of 1.41 ± 0.05 g (mean ± SD) were fed with these diets. Growth of shrimp fed BS8 and LL8 diets was significantly higher than those of shrimp fed the CON diet (p < 0.05). Superoxide dismutase activity in shrimp fed PP8 and LL8 diets was significantly higher than that of shrimp fed the CON diet (p < 0.05). Lysozyme activity of shrimp fed probiotics and OTC diets significantly improved compared to those on the CON diet (p < 0.05). The intestinal histology showed healthier guts for shrimp fed the probiotic diets (p < 0.05). Immune-related gene expression in shrimp fed BS8, PP8 and LL8 diets was recorded as significantly higher than that of shrimp fed CON and OTC diets (p < 0.05). Also, results of the challenge test for 7 days and the digestive enzyme activity of shrimp fed BS8, PP8, and LL8 were significantly improved compared to those on the CON diet (p < 0.05). Therefore, these results indicated that L. lactis at 108 CFU/g could be an ideal probiotic for whiteleg shrimp, and also B. subtilis WB60 and P. pentosaceus at 108 CFU/g could improve the growth, immunity, histology, gene expression, digestive enzyme activity, and disease resistance, while replacing antibiotics.

show abstract

Section: Discussionsupporting

confidence: 92%

Evaluation of Potential Probiotics Bacillus subtilis WB60, Pediococcus pentosaceus, and Lactococcus lactis on Growth Performance, Immune Response, Gut Histology and Immune-Related Genes in Whiteleg Shrimp, Litopenaeus vannamei

et al. 2020

View full text Add to dashboard Cite

show abstract

“…DEGs were functionally annotated against the following databases: Non-Redundant protein sequences (NR) After obtaining DEGs, candidate genes among the DEGs were identi ed. We consider candidate genes associated with ammonia tolerance when (1) candidate genes located within the QTL interval; (2) candidate genes differentially expressed between the most ammonia-tolerant and the most ammoniasensitive individuals in the mapping family (LV-N); (3) the regulation pattern (up-or down-regulated expression) of candidate genes between the most ammonia-tolerant and the most ammonia-sensitive individuals was consistent across the four families of Litopenaeus vannamei (LV-A, LV-C, LV-F, and LV-N).…”

Section: Genetic Map Construction and Qtl Analysismentioning

confidence: 99%

“…The Paci c white shrimp (Litopenaeus vannamei) is the most widely cultivated and highest-yielding crustacean species in the world [1]. L. vannamei tolerates a wide range of salinities, grows rapidly, is highly disease resistant, and can be farmed at high densities [2]. However, high-density shrimp cultivation often leads to water quality deterioration [3].…”

Section: Introductionmentioning

confidence: 99%

Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Zeng

Yang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Ammonia is one of the most common toxicological environment factors affecting shrimp health. Although ammonia tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. Results: In this study, we constructed a high-density genetic map of the Pacific white shrimp (Litopenaeus vannamei) using specific length amplified fragment sequencing (SLAF-seq). The constructed genetic map contained 17,338 polymorphic markers spanning 44 linkage groups, with a total distance of 6,360.12 centimorgans (cM) and an average distance of 0.37 cM. Using this genetic map, we identified a quantitative trait locus (QTL) that explained 7.41–8.46% of the phenotypic variance in L. vannamei survival time under acute ammonia stress. We then sequenced the transcriptomes of the most ammonia-tolerant and the most ammonia-sensitive individuals from each of four genetically distinct L. vannamei families. We found that 7546 genes were differentially expressed between the ammonia-tolerant and ammonia-sensitive individuals. Using QTL analysis and the transcriptomes, we identified one candidate gene (annotated as an ATP synthase g subunit) associated with ammonia tolerance. Conclusions: In this study, we constructed a high-density genetic map of L. vannamei and identified a QTL for ammonia tolerance. By combining QTL and transcriptome analyses, we identified a candidate gene associated with ammonia tolerance. Our work provides the basis for future genetic studies focused on molecular marker-assisted selective breeding.

show abstract

“…DEGs were functionally annotated against the following databases: Non-Redundant protein sequences (NR) After obtaining DEGs, we identified candidate genes among the DEGs. We consider candidate genes associated with ammonia tolerance when (1) candidate genes located within the QTL interval; (2) candidate genes differentially expressed between the most ammonia-tolerant and the most ammonia- Three parallel qRT-PCRs were carried out for each sample. Relative gene expression levels were calculated using the 2-ΔΔCT method [42].…”

Section: Transcriptome Sequencing Candidate Gene Identification and mentioning

confidence: 99%

QTL and transcriptomic analyses for ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Zeng¹,

Yang²,

Li³

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Ammonia is one of the most common toxicological environment factors affecting shrimp health. Although ammonia tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. Results: In this study, we constructed a high-density genetic map of the Pacific white shrimp (Litopenaeus vannamei) using specific length amplified fragment sequencing (SLAF-seq). The constructed genetic map contained 807,505 polymorphic markers spanning 44 linkage groups, with a total distance of 6,360.12 centimorgans (cM) and an average distance of 0.37 cM. Using this genetic map, we identified a quantitative trait locus (QTL) that explained 7.41–8.46% of the phenotypic variance in L. vannamei survival time under acute ammonia stress. We then sequenced the transcriptomes of the most ammonia-tolerant and the most ammonia-sensitive individuals from each of four genetically distinct L. vannamei families. We found that 7546 genes were differentially expressed between the ammonia-tolerant and ammonia-sensitive individuals. Using QTL analysis and the transcriptomes, we identified one candidate gene (annotated as an ATP synthase g subunit) associated with ammonia tolerance. Conclusions: In this study, we constructed a high-density genetic map of L. vannamei and identified a QTL for ammonia tolerance. By combining QTL and transcriptome analyses, we identified a candidate gene associated with ammonia tolerance. Our work provides the basis for future genetic studies focused on molecular marker-assisted selective breeding.

show abstract

Growth performance, immune response, and digestive enzyme activity in Pacific white shrimp, Penaeus vannamei Boone, 1931, fed dietary microbial lysozyme

Cited by 48 publications

References 58 publications

Evaluation of Potential Probiotics Bacillus subtilis WB60, Pediococcus pentosaceus, and Lactococcus lactis on Growth Performance, Immune Response, Gut Histology and Immune-Related Genes in Whiteleg Shrimp, Litopenaeus vannamei

Evaluation of Potential Probiotics Bacillus subtilis WB60, Pediococcus pentosaceus, and Lactococcus lactis on Growth Performance, Immune Response, Gut Histology and Immune-Related Genes in Whiteleg Shrimp, Litopenaeus vannamei

Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

QTL and transcriptomic analyses for ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Contact Info

Product

Resources

About