Growth performance, serum lipid profile, intestinal morphometry, and growth and lipid indicator gene expression analysis of mono-sex Nile tilapia fed Moringa oleifera leaf powder

El‐Kassas, Seham; Abdo, Safaa E.; Abosheashaa, Wesam; Mohamed, Radi A.; Moustafa, Eman M.; Helal, Mohamed; El-Naggar, Karima

doi:10.1016/j.aqrep.2020.100422

Cited by 33 publications

(39 citation statements)

References 34 publications

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“…The decrease in the hepato‐somatic index noticed with feeding both CM and AME in the present study agrees with Gad et al (2019) and El‐Kassasa et al (2020). The hepato‐somatic index is largely implemented as a bio‐monitoring tool for hazardous effects of environmental stressors.…”

Section: Discussionsupporting

confidence: 93%

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Investigations on the influence of Moringa oleifera on the growth, haematology, immunity and disease resistance in Oreochromis niloticus with special reference to the analysis of antioxidant activities by PAGE electrophoresis

Elgendy

Awad

Darwish

et al. 2021

Aquaculture Research

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The study aimed to evaluate the effect of dietary inclusions of Moringa oleifera on the growth, haematology, antioxidant activities, immunity, and protection against Aeromonas hydrophila in Oreochromis niloticus. Diets supplemented with 0.5% or 1% of either crude moringa (CM) powder or alcoholic moringa extracts (AME) were used.Weight gain (WG), specific growth rate (SGR), and haematological parameters (RBCs count, Hb content and HCT %) were significantly improved (p < 0.05) with feeding CM than control fish, meanwhile they decreased significantly (p < 0.05) in AME treatments. The feeding of CM significantly improved WBCs and lymphocyte counts (p < 0.05). Cholesterol and triglycerides showed no significant changes (p > 0.05) in CM treatments, but they significantly increased in fish fed 0.5% AME. Liver and kidney functions (ALT, AST, uric acid and creatinine) were not relatively affected by moringa treatments. The non-specific immune responses of fish fed CM showed significant (p < 0.05) improvement, including serum proteins, lysozyme activity, and antiprotease activity. Peroxidase activity (POX) and Cytochrome P450 (CYP) were increased significantly (p ˂ 0.05) with CM and in 0.5% AME treatment. Superoxide dismutase (SOD) and Catalase (CAT) showed insignificant changes (p > 0.05). Antioxidant activities were also analysed by staining on polyacrylamide gel electrophoresis. Challenge experiment indicated higher survivability of fish fed moringa-supplemented diets.The survivability was found the highest (80%) in the group fed with 0.5% CM. These results indicate that dietary supplementation with crude M. oleifera enhances the growth, antioxidant activities, immune competence and potentiates resistance against A. hydrophila in O. niloticus, with the greatest effects of 0.5% crude M. oleifera.

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Section: Discussionsupporting

confidence: 93%

“…Variations of enzymatic activities are linked to the intensities of the corresponding bands (Ostapchenko et al, 2011). Crude M. oleifera has been investigated for its valuable outcomes in some aquatic animal species (Elabd et al, 2019;El-Kassasa et al, 2020;Gad et al, 2019). Studies in O. niloticus have shown mixed findings.…”

Section: Introductionmentioning

confidence: 99%

Investigations on the influence of Moringa oleifera on the growth, haematology, immunity and disease resistance in Oreochromis niloticus with special reference to the analysis of antioxidant activities by PAGE electrophoresis

Elgendy

Awad

Darwish

et al. 2021

Aquaculture Research

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“…Given their high content of protein (up to 30% DM) and low content of antinutritional factors, MOLs are widely used in animal husbandry, including poultry, ruminants, nonruminants, and aquatic animals. Previous studies have also shown that MOLs played important roles in promoting growth, enhancing immunity, increasing milk production, improving meat and egg quality, and resistance to heat stress [ 5 , 6 , 7 , 8 ]. With such characteristics, MOLs could alleviate the problems of feed shortage and antibiotics abuse to some extent [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage

et al. 2021

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The present study investigated the effects of malic acid, sucrose, and their mixture on the fermentation parameters, Cornell Net Carbohydrate and Protein System (CNCPS) nitrogen fractions, and bacterial community of Moringa oleifera leaves (MOL) silages. The trial was divided into four treatments and labeled as CON (control group) and MLA, SUC, and MIX (respectively denoting the addition of 1% malic acid, 1% sucrose, and 1% malic acid + 1% sucrose to the fresh weight basis). The silage packages were opened on the 2nd, 5th, 10th, 20th, and 40th days of ensiling for subsequent determination. Malic acid and sucrose increased the lactic acid content (p < 0.05) and pH value, and the acetic acid contents of MLA and MIX were lower than those in CON (p < 0.05). Compared with sucrose, malic acid had a better capacity to preserve nutrients and inhibit proteolysis, and thus exerted better effects on the CNCPS nitrogen fractions. The results of 16S rRNA showed that the dominant phyla were Firmicutes and Proteobacteria and that the dominant genera were Lactobacillus and Weissella. With the application of silage additives and the processing of fermentation, there was a remarkable change in the composition and function of the bacterial community. The variation of the fermentation parameters and CNCPS nitrogen fractions in the MOL silages caused by malic acid and sucrose might be attributed to the dynamic and dramatic changes of the bacterial community.

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“…Gene-specific primer sequences for growth hormone receptor (GHR), insulin-like growth factor (IGF), fatty acid synthetase (FAS) and lipoprotein lipase (LPL) are listed in Table 2. Real-time PCR was conducted according to (El-Kassas et al, 2020). All real-time PCRs were performed in duplicate.…”

Section: Total Rna Extraction and Real-time Quantitative Pcr Assaymentioning

confidence: 99%

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confidence: 99%

Plant Ingredient diet supplemented with lecithin as fish meal and fish oil alternative affects growth performance, serum biochemical, lipid metabolism and growth‐related gene expression in Nile tilapia

et al. 2021

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Nile tilapia (Oreochromis niloticus) are valuable fish in the aquaculture sector because of their rapid growth rate, disease resistance and adaptation to a wide range of environmental conditions (El-Kassas et al., 2020;El-Sayed, 2006). The rapid expansion of this sector including Nile tilapia farming has come to a corresponding increase in food demand (Tran-Ngoc et al., 2019). Fish meal (FM) and fish oil (FO) are considered the main traditional protein and oil sources used in aquaculture diets respectively (El-Saidy & Gaber, 2003;Thompson et al., 2012), because they contain essential nutrients, have a balanced amino acid composition, with suitability for most of the aquaculture species. However, with a marked increase in their price and fluctuations in supply around the world, several studies have been conducted to evaluate possible (complete or partial) replacements, with other alternatives such as plant protein and oil sources, which

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Growth performance, serum lipid profile, intestinal morphometry, and growth and lipid indicator gene expression analysis of mono-sex Nile tilapia fed Moringa oleifera leaf powder

Cited by 33 publications

References 34 publications

Investigations on the influence of Moringa oleifera on the growth, haematology, immunity and disease resistance in Oreochromis niloticus with special reference to the analysis of antioxidant activities by PAGE electrophoresis

Investigations on the influence of Moringa oleifera on the growth, haematology, immunity and disease resistance in Oreochromis niloticus with special reference to the analysis of antioxidant activities by PAGE electrophoresis

Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage

Plant Ingredient diet supplemented with lecithin as fish meal and fish oil alternative affects growth performance, serum biochemical, lipid metabolism and growth‐related gene expression in Nile tilapia

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