Growth rate induced high efficient light trapping/photon conversion ZnO:Nd 3+ nanodisk shaped thin films deposited by AACVD process

Salhi, Rached; Deschanvres, Jean‐Luc; Gucciardi, P. G.; Elleuch, Riadh

doi:10.1016/j.jallcom.2015.08.157

Cited by 4 publications

(1 citation statement)

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“…The next challenge is to adapt the assay to a device for onsite monitoring and a recently developed hand-held fluorescent monitor for bacterial detection may prove suitable (Heery et al, 2016). One major recognised threat to biodiversity in aquatic systems is that of invasive species (Molnar, Gamboa, Revenga, & Spalding, 2008). Detection of these species using traditional methods may be slow, enabling their establishment prior to detection.…”

Section: Discussionmentioning

confidence: 99%

The application of CRISPR‐Cas for single species identification from environmental DNA

Williams

O’Grady

Ball

et al. 2019

Molecular Ecology Resources

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We report the first application of CRISPR‐Cas technology to single species detection from environmental DNA (eDNA). Organisms shed and excrete DNA into their environment such as in skin cells and faeces, referred to as environmental DNA (eDNA). Utilising eDNA allows noninvasive monitoring with increased specificity and sensitivity. Current methods primarily employ PCR‐based techniques to detect a given species from eDNA samples, posing a logistical challenge for on‐site monitoring and potential adaptation to biosensor devices. We have developed an alternative method; coupling isothermal amplification to a CRISPR‐Cas12a detection system. This utilises the collateral cleavage activity of Cas12a, a ribonuclease guided by a highly specific single CRISPR RNA. We used the target species Salmo salar as a proof‐of‐concept test of the specificity of the assay among closely related species and to show the assay is successful at a single temperature of 37°C with signal detection at 535 nM. The specific assay, detects at attomolar sensitivity with rapid detection rates (<2.5 hr). This approach simplifies the challenge of building a biosensor device for rapid target species detection in the field and can be easily adapted to detect any species from eDNA samples from a variety of sources enhancing the capabilities of eDNA as a tool for monitoring biodiversity.

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Section: Discussionmentioning

confidence: 99%